FastQCFastQC Report
Thu 26 May 2016
SRR805427_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805427_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7171006
Sequences flagged as poor quality0
Sequence length53
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGAACTTTTTTTTTTTTTTTTTTTTTTTTTTTT81890.11419597194591666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT267600.041.6343421
TCAACGC285400.039.07060212
CAACGCA286500.038.89598513
ATCAACG290250.038.4744211
ACGCAGA289850.038.4707615
AACGCAG290450.038.43174414
GTGGTAT289950.038.319716
AGTGGTA290800.038.2238665
GTATCAA291700.038.2106639
GGTATCA292900.038.0541158
TGGTATC293400.037.9011577
CGCAGAG294350.037.8826216
TATCAAC297600.037.45312510
AGAGTAC256150.037.44954319
GCAGTGG300450.037.0431023
AGCAGTG300800.036.9843752
CAGTGGT304300.036.504934
ACATGGG183000.036.3800524
GTACATG163050.035.7724622
GAGTACT104400.035.3850620
Produced by FastQC (version 0.11.5)