##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805427_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7171006
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.39143071418432	37.0	35.0	37.0	33.0	37.0
2	35.7110020546629	37.0	35.0	37.0	35.0	37.0
3	37.65080743203952	39.0	37.0	39.0	35.0	39.0
4	37.75785824192589	39.0	37.0	39.0	35.0	39.0
5	37.711263384802635	39.0	37.0	39.0	35.0	39.0
6	37.58528970691142	39.0	37.0	39.0	35.0	39.0
7	37.641940754198224	39.0	37.0	39.0	35.0	39.0
8	38.64197673241384	40.0	39.0	40.0	36.0	40.0
9	38.60599516999428	40.0	39.0	40.0	36.0	40.0
10	38.579150261483534	40.0	38.0	40.0	36.0	40.0
11	38.631924586313275	40.0	39.0	40.0	36.0	40.0
12	38.681078219708645	40.0	39.0	40.0	36.0	40.0
13	38.681286140326755	40.0	39.0	40.0	36.0	40.0
14	38.706770430815425	40.0	39.0	40.0	36.0	40.0
15	38.67839184627652	40.0	39.0	40.0	36.0	40.0
16	38.64539354729308	40.0	39.0	40.0	36.0	40.0
17	38.57543934560925	40.0	39.0	40.0	36.0	40.0
18	38.51143591289702	40.0	39.0	40.0	35.0	40.0
19	38.43889169804069	40.0	38.0	40.0	35.0	40.0
20	38.35642572325278	40.0	38.0	40.0	35.0	40.0
21	38.39637228026305	40.0	38.0	40.0	35.0	40.0
22	38.338654158147406	40.0	38.0	40.0	35.0	40.0
23	33.50117263881804	40.0	36.0	40.0	3.0	40.0
24	22.26124953737314	18.0	3.0	40.0	3.0	40.0
25	28.81133469976179	32.0	16.0	40.0	16.0	40.0
26	34.05114066283029	36.0	30.0	40.0	25.0	40.0
27	36.46766339339278	38.0	35.0	40.0	30.0	40.0
28	37.14314253815992	39.0	37.0	40.0	33.0	40.0
29	37.44141463554765	40.0	37.0	40.0	33.0	40.0
30	37.6616642072256	40.0	38.0	40.0	33.0	40.0
31	37.725310088988905	40.0	38.0	40.0	33.0	40.0
32	37.73824007398683	40.0	38.0	40.0	33.0	40.0
33	37.79514896515217	40.0	38.0	40.0	33.0	40.0
34	37.81535784518936	40.0	38.0	40.0	33.0	40.0
35	37.54504625989715	40.0	38.0	40.0	33.0	40.0
36	37.72623213535172	40.0	38.0	40.0	33.0	40.0
37	37.57177807409449	40.0	37.0	40.0	33.0	40.0
38	37.62016305661995	40.0	37.0	40.0	33.0	40.0
39	37.51023078212457	40.0	37.0	40.0	33.0	40.0
40	37.510895542410644	40.0	37.0	40.0	33.0	40.0
41	37.48958751394156	40.0	37.0	40.0	33.0	40.0
42	37.458183830832105	40.0	37.0	40.0	33.0	40.0
43	37.46080131574287	40.0	37.0	40.0	33.0	40.0
44	37.25908735817541	40.0	37.0	40.0	32.0	40.0
45	37.267514488204306	40.0	37.0	40.0	32.0	40.0
46	37.108613631058184	40.0	36.0	40.0	32.0	40.0
47	36.94296072266569	40.0	36.0	40.0	31.0	40.0
48	36.93818524207064	39.0	36.0	40.0	31.0	40.0
49	36.76235217206624	39.0	36.0	40.0	31.0	40.0
50	36.67746798705788	39.0	35.0	40.0	31.0	40.0
51	36.560480914393324	39.0	35.0	40.0	31.0	40.0
52	36.33535545779769	39.0	35.0	40.0	30.0	40.0
53	35.98296236260296	38.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	9.0
14	35.0
15	95.0
16	268.0
17	547.0
18	1008.0
19	1926.0
20	3315.0
21	5435.0
22	8550.0
23	12976.0
24	18693.0
25	25980.0
26	34752.0
27	46066.0
28	59872.0
29	77539.0
30	99615.0
31	126439.0
32	162046.0
33	212635.0
34	288147.0
35	413994.0
36	656686.0
37	1383404.0
38	1817174.0
39	1713797.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.854749807767556	30.041572967586415	20.066752140494653	35.03692508415137
2	23.30166785524932	27.15383308841186	27.407995475111864	22.136503581226957
3	20.00540788837717	24.003828751502926	30.283505549988384	25.707257810131523
4	18.091087917092803	26.575755200874184	29.677802528682868	25.655354353350145
5	22.619476263163076	29.130403739726336	28.16703820914388	20.08308178796671
6	24.53316870743101	25.235524834311953	28.697340373163822	21.53396608509322
7	23.24435372108181	26.196603377545635	29.786838834049224	20.77220406732333
8	24.07104386748526	25.004971408474624	28.19559766091396	22.72838706312615
9	24.055034956043826	24.452203777266398	29.29017211811007	22.20258914857971
10	21.98168569375064	25.58168268162096	29.75093034366447	22.685701280963926
11	21.82403696217797	29.007282939102268	26.678962477510126	22.489717621209632
12	21.88052833870171	25.84415631502749	29.4912875543543	22.784027791916504
13	22.195937362205527	26.189742415499307	25.822081309093868	25.7922389132013
14	22.43799545001078	29.383492357975992	25.765631767704562	22.412880424308668
15	22.1474504414025	29.077510184763476	25.884248318855125	22.890791054978894
16	22.612629246161557	25.83933132952336	25.67758275477667	25.870456669538417
17	26.007968756406	25.6184418197391	25.67894936916801	22.694640054686886
18	22.431232103278116	25.860346512051446	25.673580526916307	26.034840857754126
19	22.987374435330274	28.82760103673041	25.557780874817286	22.62724365312203
20	25.954838135681378	25.833097894493463	25.675365492651935	22.536698477173218
21	22.825374849777006	28.815608298194146	25.81522034704754	22.54379650498131
22	25.772255663989124	25.451143117158175	25.89986676904189	22.876734449810808
23	19.622658243487734	35.50374940419796	25.271837730996182	19.601754621318122
24	14.11401691757056	55.260056957141025	16.21450323706325	14.411422888225168
25	22.5632219524011	25.6251214962029	25.78501537998992	26.02664117140608
26	22.671979356871265	27.496100268218992	27.35313566883085	22.478784706078898
27	22.858578001468693	25.363512455574572	29.15330150330372	22.624608039653012
28	24.95761403630118	25.370986999592525	26.908721035793302	22.76267792831299
29	24.904706536293514	25.54580208132583	26.962311842996645	22.587179539384014
30	24.674125220366573	25.441646541642832	27.081067844595303	22.80316039339529
31	24.05196704618571	25.715039145135286	26.991443041603922	23.241550767075083
32	23.638036281101982	26.189742415499307	27.146107533587337	23.026113769811378
33	23.436349098020557	25.916405592186088	27.328341379159355	23.318903930634
34	23.43622359261727	25.87062401007613	27.22779760608205	23.465354791224552
35	23.76114313668124	25.905723687861926	27.332650398005526	23.00048277745131
36	23.298627835480822	25.915025032749938	27.42873175674375	23.357615375025485
37	23.498000141123853	25.8731898983211	27.187594041895935	23.44121591865911
38	23.63627920545597	26.04414220264214	27.278557569189037	23.04102102271285
39	23.458716949895177	25.960234868022702	27.364821616381303	23.216226565700822
40	23.458298598550886	26.04058621621569	27.087147884132296	23.41396730110113
41	23.481391592755603	26.214550650215607	27.21192814508871	23.09212961194008
42	23.400831068890472	26.0214536147369	27.380607407105785	23.197107909266844
43	23.384264355656654	25.95248142310856	27.28237851146687	23.380875709767917
44	23.52257131007839	25.98433190545371	27.266146479308485	23.226950305159416
45	23.441913170899593	25.812222162413473	27.393980705078196	23.35188396160873
46	23.412935367785217	25.880050860367433	27.093409209251814	23.61360456259554
47	23.490483761971472	25.9931591188182	27.22809045202305	23.288266667187283
48	23.28259103394977	25.87491908387749	27.35794670929016	23.484543172882578
49	23.366609928927684	26.05680430332927	27.025385280670523	23.551200487072524
50	23.654351983529228	26.09417702341903	27.087273389535582	23.16419760351616
51	23.440379215970538	25.955758508638816	27.220601962960288	23.383260312430362
52	23.587081087367658	25.965840776036163	26.875448158877568	23.571629977718608
53	23.685407598320236	26.153694474666455	26.81169141400802	23.34920651300529
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2532.0
1	1706.0
2	880.0
3	742.0
4	604.0
5	638.5
6	673.0
7	821.5
8	1174.0
9	1378.0
10	1859.0
11	2340.0
12	3461.5
13	4583.0
14	6014.5
15	7446.0
16	10205.0
17	12964.0
18	17464.5
19	21965.0
20	31029.0
21	40093.0
22	82596.5
23	125100.0
24	106822.0
25	97434.5
26	106325.0
27	119063.5
28	131802.0
29	145966.5
30	160131.0
31	174965.0
32	189799.0
33	208737.5
34	227676.0
35	249509.5
36	271343.0
37	292968.0
38	314593.0
39	335530.0
40	356467.0
41	378487.0
42	419646.0
43	438785.0
44	456369.5
45	473954.0
46	485228.0
47	496502.0
48	501498.0
49	506494.0
50	504202.0
51	501910.0
52	490873.5
53	479837.0
54	456653.0
55	433469.0
56	408454.0
57	383439.0
58	344763.5
59	268979.5
60	231871.0
61	200325.0
62	168779.0
63	141902.0
64	115025.0
65	93767.0
66	72509.0
67	57796.5
68	43084.0
69	33596.0
70	24108.0
71	18453.5
72	12799.0
73	9609.5
74	6420.0
75	4973.0
76	2677.0
77	1828.0
78	1375.0
79	922.0
80	743.5
81	565.0
82	389.0
83	213.0
84	148.0
85	83.0
86	58.5
87	34.0
88	31.0
89	28.0
90	20.5
91	13.0
92	8.5
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	7171006.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.55035340104787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.41527903668904	74.70678162968677
2	7.215654163695835	12.057409107930592
3	1.7680866991568593	4.431728056747435
4	0.6637058763125118	2.218114420810501
5	0.33778782383022876	1.4111146027793258
6	0.17981768255829753	0.9014298555301922
7	0.10941042142341328	0.6398895562978631
8	0.07193750343686475	0.4808323067951317
9	0.05197721284496602	0.3908443051798557
>10	0.18302924252490635	2.3033669233836496
>50	0.002575533082988602	0.14459350433331947
>100	6.511000050617052E-4	0.09654966975760286
>500	3.5084551567588937E-5	0.019038651891683415
>1k	3.507992493107547E-5	0.07830006544668383
>5k	1.7539962465537728E-5	0.12000734342932692
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGAACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8189	0.11419597194591666	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.346810754306997E-4	1.3945044809612487E-5	0.0	0.0	2.7890089619224973E-5
2	3.346810754306997E-4	1.3945044809612487E-5	0.0	1.3945044809612487E-5	2.7890089619224973E-5
3	3.4862612024031217E-4	2.7890089619224973E-5	0.0	1.3945044809612487E-5	2.7890089619224973E-5
4	3.4862612024031217E-4	2.7890089619224973E-5	0.0	1.3945044809612487E-5	5.578017923844995E-5
5	9.343180022440366E-4	2.7890089619224973E-5	0.0	1.3945044809612487E-5	6.972522404806244E-5
6	9.482630470536491E-4	2.7890089619224973E-5	0.0	1.3945044809612487E-5	1.2550540328651238E-4
7	9.482630470536491E-4	2.7890089619224973E-5	0.0	2.7890089619224973E-5	1.5339549290573735E-4
8	9.482630470536491E-4	4.183513442883746E-5	0.0	2.7890089619224973E-5	1.5339549290573735E-4
9	9.482630470536491E-4	4.183513442883746E-5	0.0	2.7890089619224973E-5	1.5339549290573735E-4
10	9.482630470536491E-4	4.183513442883746E-5	0.0	2.7890089619224973E-5	1.6734053771534985E-4
11	0.001004043226292099	4.183513442883746E-5	0.0	2.7890089619224973E-5	1.8128558252496232E-4
12	0.001031933315911324	4.183513442883746E-5	0.0	4.183513442883746E-5	1.9523062733457482E-4
13	0.001087713495149774	4.183513442883746E-5	0.0	5.578017923844995E-5	2.091756721441873E-4
14	0.0011295486295786114	4.183513442883746E-5	0.0	6.972522404806244E-5	2.231207169537998E-4
15	0.0011434936743882239	4.183513442883746E-5	0.0	2.091756721441873E-4	2.3706576176341229E-4
16	0.001171383764007449	4.183513442883746E-5	0.0	2.091756721441873E-4	2.5101080657302476E-4
17	0.0012271639432458988	4.183513442883746E-5	0.0	2.091756721441873E-4	2.5101080657302476E-4
18	0.0012271639432458988	4.183513442883746E-5	0.0	2.091756721441873E-4	2.5101080657302476E-4
19	0.0012411089880555114	4.183513442883746E-5	0.0	2.231207169537998E-4	2.5101080657302476E-4
20	0.0012411089880555114	6.972522404806244E-5	0.0	2.231207169537998E-4	2.6495585138263723E-4
21	0.0012411089880555114	8.367026885767492E-5	0.0	2.231207169537998E-4	2.6495585138263723E-4
22	0.0012550540328651238	8.367026885767492E-5	0.0	2.231207169537998E-4	3.067909858114747E-4
23	0.0012550540328651238	8.367026885767492E-5	0.0	2.231207169537998E-4	3.067909858114747E-4
24	0.0012968891672939613	8.367026885767492E-5	0.0	2.3706576176341229E-4	3.067909858114747E-4
25	0.0013108342121035737	9.761531366728741E-5	0.0	2.6495585138263723E-4	3.067909858114747E-4
26	0.0013945044809612487	9.761531366728741E-5	0.0	2.6495585138263723E-4	3.067909858114747E-4
27	0.0013945044809612487	9.761531366728741E-5	0.0	2.6495585138263723E-4	3.2073603062108717E-4
28	0.0014502846601996985	9.761531366728741E-5	0.0	2.6495585138263723E-4	3.346810754306997E-4
29	0.001492119794628536	9.761531366728741E-5	0.0	2.6495585138263723E-4	3.346810754306997E-4
30	0.0015618450186765985	1.115603584768999E-4	0.0	2.6495585138263723E-4	3.6257116504992464E-4
31	0.0015897351082958236	1.115603584768999E-4	0.0	2.6495585138263723E-4	3.6257116504992464E-4
32	0.0016455152875342735	1.115603584768999E-4	0.0	2.6495585138263723E-4	3.6257116504992464E-4
33	0.0017849657356303983	1.115603584768999E-4	0.0	2.6495585138263723E-4	4.044062994787621E-4
34	0.0018546909596784608	1.115603584768999E-4	0.0	2.6495585138263723E-4	4.183513442883746E-4
35	0.002035976542203423	1.115603584768999E-4	0.0	2.6495585138263723E-4	4.183513442883746E-4
36	0.002119646811061098	1.115603584768999E-4	0.0	2.6495585138263723E-4	4.322963890979871E-4
37	0.0022033170799187728	1.115603584768999E-4	1.3945044809612487E-5	2.6495585138263723E-4	4.462414339075996E-4
38	0.002231207169537998	1.115603584768999E-4	1.3945044809612487E-5	2.7890089619224975E-4	4.462414339075996E-4
39	0.0023009323935860603	1.115603584768999E-4	1.3945044809612487E-5	2.7890089619224975E-4	4.462414339075996E-4
40	0.0024403828416821854	1.2550540328651238E-4	1.3945044809612487E-5	2.928459410018622E-4	4.6018647871721205E-4
41	0.002551943200159085	1.2550540328651238E-4	1.3945044809612487E-5	2.928459410018622E-4	4.8807656833643704E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	26760	0.0	41.634342	1
TCAACGC	28540	0.0	39.070602	12
CAACGCA	28650	0.0	38.895985	13
ATCAACG	29025	0.0	38.47442	11
ACGCAGA	28985	0.0	38.47076	15
AACGCAG	29045	0.0	38.431744	14
GTGGTAT	28995	0.0	38.31971	6
AGTGGTA	29080	0.0	38.223866	5
GTATCAA	29170	0.0	38.210663	9
GGTATCA	29290	0.0	38.054115	8
TGGTATC	29340	0.0	37.901157	7
CGCAGAG	29435	0.0	37.88262	16
TATCAAC	29760	0.0	37.453125	10
AGAGTAC	25615	0.0	37.449543	19
GCAGTGG	30045	0.0	37.043102	3
AGCAGTG	30080	0.0	36.984375	2
CAGTGGT	30430	0.0	36.50493	4
ACATGGG	18300	0.0	36.38005	24
GTACATG	16305	0.0	35.77246	22
GAGTACT	10440	0.0	35.38506	20
>>END_MODULE