FastQCFastQC Report
Thu 26 May 2016
SRR805426_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805426_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6473432
Sequences flagged as poor quality0
Sequence length53
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGAACTTTTTTTTTTTTTTTTTTTTTTTTTTTT172210.2660258113470567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT516000.044.021511
TCAACGC531150.043.00922812
CAACGCA531700.042.98241813
GTGGTAT526000.042.800386
AACGCAG534750.042.72407514
ATCAACG535150.042.714111
ACGCAGA534500.042.68250715
AGTGGTA527950.042.65125
GTATCAA532800.042.642279
GGTATCA530500.042.623378
TGGTATC530150.042.4875037
CGCAGAG538550.042.34843416
TATCAAC537000.042.3043810
AGCAGTG537250.042.1753352
AGAGTAC442300.041.8196919
GCAGTGG542400.041.8052183
CAGTGGT541000.041.626714
ACATGGG352200.041.50866324
GAGTACT148400.040.82412720
CAGAGTA457850.040.66113318