##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805426_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6473432
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.775115889067806	35.0	35.0	37.0	32.0	37.0
2	35.11195761382834	37.0	35.0	37.0	32.0	37.0
3	36.98294984793229	39.0	37.0	39.0	33.0	39.0
4	37.102610176487524	39.0	37.0	39.0	33.0	39.0
5	37.05290238624581	39.0	37.0	39.0	33.0	39.0
6	37.026949692218906	39.0	37.0	39.0	33.0	39.0
7	36.948500424504346	39.0	37.0	39.0	33.0	39.0
8	37.77684109449207	40.0	38.0	40.0	33.0	40.0
9	37.90837719466274	40.0	38.0	40.0	33.0	40.0
10	37.702986298458065	40.0	37.0	40.0	33.0	40.0
11	37.888996130646	40.0	38.0	40.0	33.0	40.0
12	38.02335469037135	40.0	38.0	40.0	34.0	40.0
13	38.09442363803312	40.0	38.0	40.0	34.0	40.0
14	38.03923977265846	40.0	38.0	40.0	34.0	40.0
15	38.031674697440245	40.0	38.0	40.0	34.0	40.0
16	37.963794321157614	40.0	38.0	40.0	34.0	40.0
17	37.925281519910925	40.0	38.0	40.0	33.0	40.0
18	37.85542444873137	40.0	38.0	40.0	33.0	40.0
19	37.80247386548588	40.0	38.0	40.0	33.0	40.0
20	37.67070033330079	40.0	38.0	40.0	33.0	40.0
21	37.63225936412092	40.0	37.0	40.0	33.0	40.0
22	37.559438331938914	40.0	37.0	40.0	33.0	40.0
23	31.491760784696588	39.0	31.0	40.0	3.0	40.0
24	23.567002789246878	30.0	3.0	40.0	3.0	40.0
25	29.4009372462706	32.0	17.0	40.0	16.0	40.0
26	33.693889732679665	36.0	30.0	40.0	25.0	40.0
27	35.73068721506613	38.0	34.0	40.0	30.0	40.0
28	36.29668327403455	39.0	36.0	40.0	30.0	40.0
29	36.6384721427521	39.0	36.0	40.0	30.0	40.0
30	36.873983846590185	39.0	36.0	40.0	30.0	40.0
31	36.95962141874666	40.0	37.0	40.0	30.0	40.0
32	36.91076989763699	40.0	37.0	40.0	30.0	40.0
33	36.920698634047596	40.0	37.0	40.0	30.0	40.0
34	36.96884573746971	40.0	37.0	40.0	31.0	40.0
35	36.6027210913778	40.0	36.0	40.0	30.0	40.0
36	36.78091451335242	40.0	36.0	40.0	30.0	40.0
37	36.61918191154244	39.0	36.0	40.0	30.0	40.0
38	36.55866548069092	39.0	35.0	40.0	30.0	40.0
39	36.51705602221511	39.0	35.0	40.0	30.0	40.0
40	36.50471558208999	39.0	35.0	40.0	30.0	40.0
41	36.4827565965009	39.0	35.0	40.0	30.0	40.0
42	36.38654982395737	39.0	35.0	40.0	30.0	40.0
43	36.38781607654178	39.0	35.0	40.0	30.0	40.0
44	36.257805442306335	39.0	35.0	40.0	30.0	40.0
45	36.1987991841113	39.0	35.0	40.0	29.0	40.0
46	36.08602361158656	39.0	35.0	40.0	29.0	40.0
47	35.89055156522846	39.0	35.0	40.0	28.0	40.0
48	35.826817984648635	39.0	35.0	40.0	28.0	40.0
49	35.63200324032136	38.0	34.0	40.0	28.0	40.0
50	35.554197526134516	38.0	34.0	40.0	28.0	40.0
51	35.42155876511872	38.0	34.0	40.0	27.0	40.0
52	35.134863083446305	38.0	34.0	40.0	26.0	40.0
53	34.715113559546154	37.0	33.0	40.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	16.0
11	41.0
12	72.0
13	151.0
14	283.0
15	625.0
16	1137.0
17	2057.0
18	3535.0
19	5639.0
20	9255.0
21	13704.0
22	19630.0
23	27565.0
24	37289.0
25	48669.0
26	61765.0
27	78044.0
28	96643.0
29	118021.0
30	142364.0
31	171750.0
32	208460.0
33	255580.0
34	322839.0
35	432603.0
36	626616.0
37	1108030.0
38	1317228.0
39	1363818.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.159892310601238	33.680140611657	29.324892885257775	22.835074192483987
2	26.10321696435523	36.345573723490105	18.535345702248822	19.015863609905843
3	31.73335257093919	25.032332153948634	21.099302502907268	22.135012772204917
4	21.989278639213325	24.219857411030194	22.192864619571196	31.59799933018529
5	22.37335620425147	31.946438921425298	23.95163801828767	21.72856685603556
6	30.43886148800204	24.1188136370321	22.785749506598663	22.656575368367196
7	23.078932473531815	23.699546083128702	31.500292271549313	21.72122917179017
8	29.4874959681356	24.89129413887409	23.91023803138737	21.710971861602935
9	29.27284939426258	24.394772355683973	24.48055374645165	21.8518245036018
10	21.707125370282718	24.78682096297605	31.535822111053303	21.970231555687924
11	21.62253345675061	32.16680116513157	24.26691127673852	21.943754101379298
12	21.51232916326301	24.635340264638604	31.710088249942224	22.14224232215616
13	21.5173805795751	24.710617181118145	24.30245656399882	29.469545675307934
14	21.936246491814543	32.292793065563984	23.991261513212773	21.779698929408696
15	21.724797603496878	31.9789564484496	24.027934486683417	22.268311461370104
16	21.916164408616634	24.414869886638186	24.161202280335996	29.507763424409184
17	29.63091293768128	24.222421738577	24.115924906602864	22.03074041713885
18	21.963048349005597	24.460255394665456	24.05295985189927	29.523736404429673
19	22.150491424023606	31.85878835214458	24.092490660286536	21.89822956354527
20	29.741905066740486	24.383495493580533	24.124884605260394	21.74971483441859
21	22.19632491698376	31.783279719320447	24.098886031397257	21.921509332298537
22	29.43027747877787	24.19818420893276	24.392872281658324	21.978666030631047
23	18.62211574942009	36.53797552828237	26.43718200793644	18.4027267143611
24	15.431273550104487	52.193504156682266	16.832709449948652	15.542512843264594
25	21.977183046025665	24.331560136879478	24.21746300880275	29.473793808292108
26	22.264434074537277	29.176872484332883	26.65400671544862	21.904686725681216
27	22.441357227510846	24.108633565626395	31.52300047331925	21.927008733543506
28	27.435431468191833	24.077351859106578	26.601129045612897	21.886087627088692
29	27.421930129180318	24.187756973426154	26.48344803807316	21.906864859320375
30	27.23794735157487	24.15463698390591	26.517618475022214	22.08979718949701
31	25.175625541443857	24.998053582705435	27.105405602468675	22.720915273382033
32	24.167520412665183	25.74513797318022	27.251216974241792	22.8361246399128
33	23.889306321592628	25.300057218489357	27.574322245139825	23.23631421477819
34	23.496191819115424	25.205841352778556	27.8678141671991	23.43015266090692
35	23.89418781258535	25.185017777277956	27.860862676861366	23.05993173327533
36	23.468849290453658	25.324402882427744	27.85522424580964	23.351523581308957
37	23.70251205233947	25.231963508692147	27.86643931688786	23.19908512208053
38	23.882725577406237	25.374484508372063	27.691292655889487	23.05149725833221
39	23.83482208510107	25.225012018354402	27.766198826217686	23.173967070326835
40	23.553178592128564	25.435703966613072	27.637549911700628	23.373567529557736
41	23.764287629807495	25.652065241436073	27.474390709595774	23.109256419160655
42	23.7310286104805	25.51834946285062	27.584425077764006	23.166196848904878
43	23.620175511228048	25.3707615991023	27.934069593995893	23.074993295673764
44	23.78877232355264	25.28286386572069	27.66980482686773	23.258558983858947
45	23.783535534164876	25.06010721978697	27.71098854517974	23.44536870086841
46	23.629397821742778	25.20099075729845	27.572792917265527	23.59681850369325
47	23.791027695973327	25.256463650193588	27.53111487075171	23.42139378308137
48	23.67518497143401	25.181820091722596	27.571093664071856	23.571901272771537
49	23.48028063012016	25.54773109534479	27.524256066951814	23.44773220758324
50	23.99033464783441	25.39890741109198	27.36715238531895	23.24360555575466
51	23.771270015657848	25.256015665260716	27.48674891464064	23.485965404440797
52	23.838112457194267	25.32681273241149	27.353187613618246	23.48188719677599
53	24.176541902347935	25.41583815200345	26.95378587432447	23.453834071324145
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	788.0
1	516.5
2	245.0
3	209.0
4	173.0
5	205.0
6	237.0
7	272.0
8	401.5
9	496.0
10	762.0
11	1028.0
12	1557.0
13	2086.0
14	2802.0
15	3518.0
16	5118.0
17	6718.0
18	9601.0
19	12484.0
20	18096.0
21	23708.0
22	86564.5
23	149421.0
24	111574.5
25	76265.5
26	78803.0
27	87305.5
28	95808.0
29	106143.5
30	116479.0
31	128007.0
32	139535.0
33	156364.0
34	173193.0
35	193120.5
36	213048.0
37	235321.0
38	257594.0
39	279135.5
40	300677.0
41	322488.0
42	365036.5
43	385774.0
44	406163.5
45	426553.0
46	440529.0
47	454505.0
48	465417.0
49	476329.0
50	478121.0
51	479913.0
52	471709.5
53	463506.0
54	446243.5
55	428981.0
56	407138.0
57	385295.0
58	346378.0
59	269051.5
60	230642.0
61	198378.0
62	166114.0
63	139464.0
64	112814.0
65	91866.0
66	70918.0
67	56584.5
68	42251.0
69	32837.5
70	23424.0
71	17735.5
72	12047.0
73	9135.0
74	6223.0
75	4713.0
76	2405.0
77	1607.0
78	1200.0
79	793.0
80	605.0
81	417.0
82	295.0
83	173.0
84	113.5
85	54.0
86	39.0
87	24.0
88	22.5
89	21.0
90	18.0
91	15.0
92	8.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	6473432.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.24983288014487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.54504699593272	67.00180845271662
2	11.476753892482321	18.190616559715522
3	2.1500187811953917	5.111658872967226
4	0.7650665901831714	2.4252559765679442
5	0.3748326118970716	1.4852710925435564
6	0.2061063764342308	0.9800337532767012
7	0.13305402798368715	0.7381156637216126
8	0.0820659481711642	0.5202970142172314
9	0.061423094366908786	0.43809929672029996
>10	0.20184078126874033	2.4582157100078716
>50	0.002841375456820969	0.1551964864220399
>100	7.592032906128077E-4	0.0853956446453017
>500	1.0573473154929763E-4	0.059996804553095195
>1k	6.343995428920663E-5	0.061437342557225556
>5k	0.0	0.0
>10k+	2.1146651369442112E-5	0.28860132936778216
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGAACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17221	0.2660258113470567	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.634326891824924E-5	0.0	0.0	0.0	4.634326891824924E-5
2	1.0813429414258155E-4	0.0	0.0	0.0	4.634326891824924E-5
3	1.0813429414258155E-4	0.0	0.0	0.0	7.72387815304154E-5
4	1.0813429414258155E-4	0.0	0.0	0.0	9.268653783649848E-5
5	1.2358205044866462E-4	0.0	0.0	0.0	1.0813429414258155E-4
6	1.544775630608308E-4	0.0	0.0	0.0	1.0813429414258155E-4
7	1.544775630608308E-4	0.0	0.0	0.0	1.0813429414258155E-4
8	1.544775630608308E-4	0.0	0.0	0.0	1.2358205044866462E-4
9	1.544775630608308E-4	0.0	0.0	0.0	1.2358205044866462E-4
10	1.544775630608308E-4	0.0	0.0	0.0	1.2358205044866462E-4
11	1.544775630608308E-4	0.0	0.0	0.0	1.2358205044866462E-4
12	1.6992531936691388E-4	0.0	0.0	3.0895512612166156E-5	1.2358205044866462E-4
13	1.6992531936691388E-4	0.0	0.0	9.268653783649848E-5	1.2358205044866462E-4
14	1.6992531936691388E-4	0.0	0.0	2.162685882851631E-4	1.2358205044866462E-4
15	2.0082083197908002E-4	0.0	0.0	3.552983950399108E-4	1.2358205044866462E-4
16	2.162685882851631E-4	0.0	0.0	3.7074615134599393E-4	1.544775630608308E-4
17	2.317163445912462E-4	0.0	0.0	3.7074615134599393E-4	1.544775630608308E-4
18	2.317163445912462E-4	0.0	0.0	3.7074615134599393E-4	1.544775630608308E-4
19	2.4716410089732925E-4	0.0	0.0	3.7074615134599393E-4	1.544775630608308E-4
20	2.6261185720341236E-4	4.634326891824924E-5	0.0	3.7074615134599393E-4	1.8537307567299696E-4
21	2.780596135094954E-4	6.179102522433231E-5	0.0	3.7074615134599393E-4	1.8537307567299696E-4
22	3.3985063873382776E-4	6.179102522433231E-5	0.0	3.7074615134599393E-4	1.8537307567299696E-4
23	3.552983950399108E-4	6.179102522433231E-5	0.0	3.7074615134599393E-4	2.162685882851631E-4
24	4.0164166395816004E-4	6.179102522433231E-5	0.0	3.7074615134599393E-4	2.162685882851631E-4
25	4.1708942026424316E-4	6.179102522433231E-5	0.0	3.7074615134599393E-4	2.162685882851631E-4
26	4.1708942026424316E-4	6.179102522433231E-5	0.0	3.7074615134599393E-4	2.6261185720341236E-4
27	4.1708942026424316E-4	6.179102522433231E-5	0.0	3.7074615134599393E-4	2.6261185720341236E-4
28	4.943282017946585E-4	6.179102522433231E-5	0.0	3.7074615134599393E-4	2.9350736981557853E-4
29	5.252237144068247E-4	6.179102522433231E-5	0.0	3.7074615134599393E-4	2.9350736981557853E-4
30	5.406714707129077E-4	6.179102522433231E-5	0.0	3.7074615134599393E-4	2.9350736981557853E-4
31	5.870147396311571E-4	6.179102522433231E-5	0.0	3.7074615134599393E-4	2.9350736981557853E-4
32	6.642535211615724E-4	6.179102522433231E-5	0.0	3.7074615134599393E-4	2.9350736981557853E-4
33	7.569400589980709E-4	6.179102522433231E-5	0.0	3.7074615134599393E-4	2.9350736981557853E-4
34	8.187310842224032E-4	6.179102522433231E-5	0.0	3.7074615134599393E-4	2.9350736981557853E-4
35	9.577608909771509E-4	6.179102522433231E-5	0.0	3.7074615134599393E-4	3.7074615134599393E-4
36	0.0010349996725075663	6.179102522433231E-5	0.0	3.7074615134599393E-4	3.7074615134599393E-4
37	0.001143133966650148	6.179102522433231E-5	0.0	3.7074615134599393E-4	3.86193907652077E-4
38	0.0013285070423231448	6.179102522433231E-5	0.0	3.7074615134599393E-4	4.0164166395816004E-4
39	0.0014675368490778926	6.179102522433231E-5	0.0	3.7074615134599393E-4	4.0164166395816004E-4
40	0.0016683576810569726	6.179102522433231E-5	0.0	3.7074615134599393E-4	4.0164166395816004E-4
41	0.0018382830004238864	6.179102522433231E-5	0.0	3.7074615134599393E-4	4.0164166395816004E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	51600	0.0	44.02151	1
TCAACGC	53115	0.0	43.009228	12
CAACGCA	53170	0.0	42.982418	13
GTGGTAT	52600	0.0	42.80038	6
AACGCAG	53475	0.0	42.724075	14
ATCAACG	53515	0.0	42.7141	11
ACGCAGA	53450	0.0	42.682507	15
AGTGGTA	52795	0.0	42.6512	5
GTATCAA	53280	0.0	42.64227	9
GGTATCA	53050	0.0	42.62337	8
TGGTATC	53015	0.0	42.487503	7
CGCAGAG	53855	0.0	42.348434	16
TATCAAC	53700	0.0	42.30438	10
AGCAGTG	53725	0.0	42.175335	2
AGAGTAC	44230	0.0	41.81969	19
GCAGTGG	54240	0.0	41.805218	3
CAGTGGT	54100	0.0	41.62671	4
ACATGGG	35220	0.0	41.508663	24
GAGTACT	14840	0.0	40.824127	20
CAGAGTA	45785	0.0	40.661133	18
>>END_MODULE