FastQCFastQC Report
Thu 26 May 2016
SRR805425_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805425_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13717752
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT657680.4794371555922574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT430950.040.532661
TCAACGC484250.036.21208212
CAACGCA484450.036.19228413
AGTGGTA482850.036.1662035
GTGGTAT485250.035.9970136
GGTATCA485800.035.9852838
GTATCAA488150.035.768729
TGGTATC488650.035.7128837
AACGCAG492400.035.6461214
ATCAACG491700.035.620411
ACGCAGA493900.035.4712515
TATCAAC500150.034.9246210
CGCAGAG504400.034.80273416
AGCAGTG513250.034.161422
CAGTGGT512850.034.0414354
GCAGTGG518550.033.8938373
AGAGTAC447100.033.06083719
CAGAGTA464000.032.03394318
ACATGGG368050.031.79092624
GCAGAGT480150.031.16692717
Produced by FastQC (version 0.11.5)