##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805425_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13717752
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.417985541654346	37.0	35.0	37.0	33.0	37.0
2	35.67376352918467	37.0	35.0	37.0	33.0	37.0
3	37.52103719326607	39.0	37.0	39.0	35.0	39.0
4	37.588454361910024	39.0	37.0	39.0	35.0	39.0
5	37.56550045517662	39.0	37.0	39.0	35.0	39.0
6	37.56150410067189	39.0	37.0	39.0	35.0	39.0
7	37.56990868474659	39.0	37.0	39.0	35.0	39.0
8	38.51171948581663	40.0	39.0	40.0	36.0	40.0
9	38.42358580327156	40.0	39.0	40.0	35.0	40.0
10	38.3447722338179	40.0	38.0	40.0	35.0	40.0
11	38.329591102098945	40.0	38.0	40.0	35.0	40.0
12	38.35596021855476	40.0	38.0	40.0	35.0	40.0
13	38.45018965206544	40.0	39.0	40.0	35.0	40.0
14	38.44069363551696	40.0	39.0	40.0	35.0	40.0
15	38.354078642039894	40.0	39.0	40.0	34.0	40.0
16	38.35727923933892	40.0	39.0	40.0	34.0	40.0
17	38.30592403186761	40.0	38.0	40.0	34.0	40.0
18	38.27195738777024	40.0	38.0	40.0	34.0	40.0
19	38.232310439786346	40.0	38.0	40.0	34.0	40.0
20	38.07687075841581	40.0	38.0	40.0	34.0	40.0
21	38.056841018849155	40.0	38.0	40.0	34.0	40.0
22	38.01170541645599	40.0	38.0	40.0	34.0	40.0
23	33.14333048155412	40.0	35.0	40.0	3.0	40.0
24	34.905754310181436	40.0	35.0	40.0	17.0	40.0
25	36.4667574541368	40.0	35.0	40.0	30.0	40.0
26	37.19197125009987	40.0	37.0	40.0	32.0	40.0
27	37.44764244170619	40.0	37.0	40.0	33.0	40.0
28	37.48585066999316	40.0	38.0	40.0	33.0	40.0
29	37.48152109762591	40.0	38.0	40.0	32.0	40.0
30	37.359952709452685	40.0	37.0	40.0	32.0	40.0
31	37.33888118111481	40.0	37.0	40.0	32.0	40.0
32	37.47977620531411	40.0	38.0	40.0	33.0	40.0
33	37.50803666664917	40.0	38.0	40.0	33.0	40.0
34	37.469733087462146	40.0	38.0	40.0	33.0	40.0
35	37.48221253744783	40.0	38.0	40.0	33.0	40.0
36	37.43307846650092	40.0	37.0	40.0	33.0	40.0
37	37.04070630523135	40.0	37.0	40.0	31.0	40.0
38	37.069407873826556	40.0	37.0	40.0	31.0	40.0
39	37.137038998809714	40.0	37.0	40.0	31.0	40.0
40	37.038028898612545	40.0	37.0	40.0	31.0	40.0
41	36.97145866173991	40.0	37.0	40.0	31.0	40.0
42	36.897360515046486	40.0	36.0	40.0	31.0	40.0
43	36.98243043029208	40.0	36.0	40.0	31.0	40.0
44	36.74269020171818	40.0	36.0	40.0	31.0	40.0
45	36.580066981820345	39.0	36.0	40.0	30.0	40.0
46	36.54412115046255	39.0	35.0	40.0	30.0	40.0
47	36.439121074648384	39.0	35.0	40.0	30.0	40.0
48	36.384094711728274	39.0	35.0	40.0	30.0	40.0
49	36.49004158990482	39.0	35.0	40.0	30.0	40.0
50	36.472425584016975	39.0	35.0	40.0	30.0	40.0
51	36.44127405131686	39.0	35.0	40.0	30.0	40.0
52	36.37413834278386	39.0	35.0	40.0	30.0	40.0
53	35.96128148402158	38.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	11.0
11	14.0
12	93.0
13	276.0
14	581.0
15	1273.0
16	2476.0
17	4234.0
18	6890.0
19	10444.0
20	15554.0
21	22117.0
22	30542.0
23	41775.0
24	54419.0
25	70333.0
26	89621.0
27	112567.0
28	139166.0
29	168595.0
30	205377.0
31	247117.0
32	297837.0
33	363592.0
34	458471.0
35	611014.0
36	886337.0
37	1563400.0
38	2631096.0
39	5682529.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.721371548341157	36.583785739820925	16.19207724414321	19.502765467694708
2	30.11201106420352	28.852351318204324	25.492362013834335	15.543275603757817
3	25.628142278705724	24.384009858175013	25.460636699074307	24.527211164044953
4	22.739206832139843	29.775869982195335	23.369754752819556	24.115168432845266
5	23.215472914220932	30.083894212404484	22.59143480651932	24.10919806685527
6	25.180641842774236	24.745337282668473	27.465608067560925	22.608412806996363
7	22.322134122267265	25.85621900731257	27.110199980288314	24.711446890131853
8	24.530586352632707	24.815924650044703	26.399959701852023	24.253529295470567
9	25.50980656305785	25.289209194042872	25.825907918440283	23.375076324458995
10	22.691961481735493	26.453241026663843	28.360827634148798	22.493969857451862
11	22.61355942285587	28.74083158814943	26.244978040133688	22.400630948861007
12	22.657779496232326	25.26258675619737	28.605313756947933	23.47431999062237
13	22.924988000949426	25.971616923822506	25.511512381912137	25.59188269331593
14	22.90126691312104	28.51783586698462	25.58715888725791	22.993738332636426
15	23.003477537719007	27.796187013732276	25.460833524326727	23.739501924221987
16	23.72739352628623	25.45496521587502	25.04147180966677	25.776169448171977
17	25.898623914472285	25.35166111765251	25.235490479781237	23.51422448809397
18	23.22165468511167	25.48497013213244	25.14350018866065	26.14987499409524
19	23.889103695707576	27.997072698208864	24.928355608120047	23.185467997963517
20	26.017353280624988	25.669599508724172	25.175327560958966	23.137719649691874
21	23.787264852141952	27.677384749337936	25.16695155299498	23.368398845525125
22	26.198476251794027	25.30246938419648	25.29407150676	23.204982857249497
23	20.158164398948166	35.86165575817379	24.045856784697666	19.93432305818038
24	23.327109281462445	27.609633123561355	25.177536377680543	23.88572121729566
25	23.588165174585455	25.50545453803218	25.00266078581972	25.903719501562644
26	23.451080031188784	27.037965112651115	26.4403890666634	23.070565789496705
27	23.783889663554202	24.87863900732423	27.82350198487332	23.513969344248242
28	25.76230420261279	25.239827925158583	25.68186099296736	23.316006879261266
29	25.710590189996147	25.207249700971413	25.955710527497512	23.12644958153493
30	25.48233121578521	24.99319130423119	25.96508524137191	23.55939223861169
31	25.035516023325105	25.56956125172696	25.795735336227104	23.599187388720836
32	24.304944425296508	25.81374849173538	26.215672946995983	23.665634135972134
33	24.201202937624185	25.398141036519682	26.284036918002307	24.116619107853822
34	24.35684068351724	25.622215651660706	26.115488893515497	23.90545477130655
35	24.054473356859056	25.692023007851432	26.458890640390642	23.794612994898873
36	24.1753386414917	25.361334714317625	26.317796093703983	24.1455305504867
37	24.80372148439482	25.573125975742965	25.983572235450826	23.639580304411393
38	24.25371154107466	25.701565387681597	26.35448577871943	23.690237292524312
39	24.284197585726876	25.348643130448778	26.294512395325416	24.072646888498934
40	24.435804058857457	25.70862193747197	25.949521466782606	23.906052536887966
41	24.12412033691818	25.86175198385275	26.292755547701987	23.72137213152709
42	24.23224300891283	25.462896544565027	26.265899835483246	24.038960611038892
43	24.411951754194128	25.72447001520366	25.958283835427267	23.905294395174952
44	24.335153456630504	25.607708901575126	26.319006204515144	23.73813143727923
45	24.31719132989137	25.262171236220045	26.236922784432902	24.183714649455684
46	24.420918237915366	25.593807206895125	25.90243649250985	24.082838062679656
47	24.15286411359529	25.605485505205227	26.27004774543234	23.97160263576714
48	24.202216223182923	25.260939255936393	26.277877016584057	24.25896750429662
49	24.30299075242066	25.74923354788744	25.904805685363026	24.04297001432888
50	24.227439014789013	25.751281988477416	26.23520238593029	23.786076610803285
51	24.25898937376911	25.356457821952166	26.180382908219947	24.204169896058772
52	24.47586164263649	25.62710712367449	25.814907573777397	24.082123659911623
53	24.340730172115666	25.689099788361823	26.010099905582194	23.960070133940313
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2990.0
1	1994.0
2	998.0
3	951.5
4	905.0
5	912.0
6	919.0
7	1076.0
8	1514.0
9	1795.0
10	2459.5
11	3124.0
12	4551.0
13	5978.0
14	7556.0
15	9134.0
16	12605.5
17	16077.0
18	22277.0
19	28477.0
20	37890.5
21	47304.0
22	106613.5
23	165923.0
24	142417.0
25	132936.0
26	146961.0
27	166856.0
28	186751.0
29	210339.0
30	233927.0
31	258292.5
32	282658.0
33	315692.5
34	348727.0
35	388611.5
36	428496.0
37	472993.5
38	517491.0
39	560384.0
40	603277.0
41	652033.0
42	746480.0
43	792171.0
44	837353.0
45	882535.0
46	919814.5
47	957094.0
48	983647.0
49	1010200.0
50	1023712.5
51	1037225.0
52	1026212.5
53	1015200.0
54	983086.0
55	950972.0
56	901944.0
57	852916.0
58	778680.5
59	624711.5
60	544978.0
61	475295.0
62	405612.0
63	344654.0
64	283696.0
65	233728.5
66	183761.0
67	148045.5
68	112330.0
69	88004.0
70	63678.0
71	48133.5
72	32589.0
73	24840.5
74	17092.0
75	12998.5
76	6783.5
77	4662.0
78	3534.0
79	2406.0
80	1820.0
81	1234.0
82	941.0
83	648.0
84	471.0
85	294.0
86	214.0
87	134.0
88	99.0
89	64.0
90	50.0
91	36.0
92	28.0
93	13.0
94	6.0
95	4.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.3717752E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.239030656948934
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.2457650909408	46.352032769986664
2	11.952155287880704	14.160681870307586
3	3.9098523779029852	6.948475946362189
4	1.7976863047996168	4.25972776486407
5	1.0491605966960427	3.107562837586985
6	0.6447369236178705	2.291615423031958
7	0.4424053468973123	1.8345364735353606
8	0.3164610652856759	1.4997477398551098
9	0.24767560096026897	1.320485626643726
>10	1.3365032475712155	13.928674435286553
>50	0.042958174515826994	1.6897484097400117
>100	0.013927569801908552	1.5188951110374689
>500	5.014396747093728E-4	0.18858464737498604
>1k	1.3651703718936358E-4	0.13425667691229062
>5k	4.963761191699717E-5	0.20180541788690945
>10k+	2.4818805958498584E-5	0.5631688495883086
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	65768	0.4794371555922574	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4056419739910736E-4	0.0	0.0	0.0	0.0
2	2.55143845726326E-4	0.0	0.0	0.0	0.0
3	2.55143845726326E-4	0.0	0.0	0.0	7.289824163609315E-6
4	2.624336698899353E-4	0.0	0.0	0.0	2.1869472490827944E-5
5	2.624336698899353E-4	0.0	0.0	0.0	2.915929665443726E-5
6	2.624336698899353E-4	7.289824163609315E-6	0.0	0.0	2.915929665443726E-5
7	2.624336698899353E-4	7.289824163609315E-6	0.0	0.0	4.373894498165589E-5
8	2.624336698899353E-4	2.1869472490827944E-5	0.0	0.0	5.831859330887452E-5
9	2.6972349405354464E-4	2.1869472490827944E-5	0.0	0.0	7.289824163609314E-5
10	2.7701331821715396E-4	3.644912081804657E-5	0.0	0.0	8.747788996331177E-5
11	2.7701331821715396E-4	3.644912081804657E-5	0.0	0.0	1.020575382905304E-4
12	2.843031423807633E-4	3.644912081804657E-5	0.0	1.457964832721863E-5	1.020575382905304E-4
13	2.843031423807633E-4	3.644912081804657E-5	0.0	1.457964832721863E-5	1.1663718661774903E-4
14	3.3533191152602845E-4	4.373894498165589E-5	0.0	3.644912081804657E-5	1.3121683494496766E-4
15	3.426217356896378E-4	4.373894498165589E-5	0.0	3.644912081804657E-5	1.3121683494496766E-4
16	3.499115598532471E-4	4.373894498165589E-5	0.0	3.644912081804657E-5	1.530863074357956E-4
17	3.572013840168564E-4	5.10287691452652E-5	0.0	4.373894498165589E-5	1.6037613159940493E-4
18	3.7907085650768434E-4	5.831859330887452E-5	0.0	4.373894498165589E-5	1.6037613159940493E-4
19	4.155199773257309E-4	6.560841747248383E-5	0.0	4.373894498165589E-5	1.968252524174515E-4
20	4.373894498165589E-4	6.560841747248383E-5	0.0	4.373894498165589E-5	1.968252524174515E-4
21	4.519690981437775E-4	7.289824163609314E-5	0.0	4.373894498165589E-5	1.968252524174515E-4
22	4.88418218961824E-4	7.289824163609314E-5	0.0	5.10287691452652E-5	2.041150765810608E-4
23	5.029978672890427E-4	7.289824163609314E-5	0.0	5.10287691452652E-5	2.1140490074467012E-4
24	5.467368122706985E-4	7.289824163609314E-5	0.0	5.831859330887452E-5	2.1869472490827944E-4
25	6.123452297431824E-4	7.289824163609314E-5	0.0	5.831859330887452E-5	2.2598454907188874E-4
26	6.925332955428848E-4	7.289824163609314E-5	0.0	6.560841747248383E-5	2.3327437323549807E-4
27	7.945908338334153E-4	7.289824163609314E-5	0.0	6.560841747248383E-5	2.55143845726326E-4
28	8.747788996331178E-4	7.289824163609314E-5	0.0	6.560841747248383E-5	2.6972349405354464E-4
29	9.258076687783829E-4	7.289824163609314E-5	0.0	6.560841747248383E-5	2.7701331821715396E-4
30	0.0010351550312325227	7.289824163609314E-5	0.0	6.560841747248383E-5	2.7701331821715396E-4
31	0.0011445023936866624	8.018806579970246E-5	0.0	6.560841747248383E-5	2.7701331821715396E-4
32	0.0012465599319771929	8.018806579970246E-5	0.0	6.560841747248383E-5	2.988827907079819E-4
33	0.0013850665910857696	8.018806579970246E-5	0.0	6.560841747248383E-5	2.988827907079819E-4
34	0.001530863074357956	8.018806579970246E-5	0.0	6.560841747248383E-5	3.061726148715912E-4
35	0.0017568476234298448	8.018806579970246E-5	0.0	6.560841747248383E-5	3.280420873624191E-4
36	0.0019828321725017337	8.018806579970246E-5	0.0	6.560841747248383E-5	3.644912081804657E-4
37	0.0021942370732464037	8.018806579970246E-5	0.0	6.560841747248383E-5	3.644912081804657E-4
38	0.0023983521498274645	8.747788996331177E-5	0.0	7.289824163609314E-5	3.7907085650768434E-4
39	0.0025878875780813064	8.747788996331177E-5	0.0	7.289824163609314E-5	3.8636068067129367E-4
40	0.002850321247971242	8.747788996331177E-5	0.0	8.018806579970246E-5	4.009403289985123E-4
41	0.00308359562120674	8.747788996331177E-5	0.0	8.018806579970246E-5	4.082301531621216E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	43095	0.0	40.53266	1
TCAACGC	48425	0.0	36.212082	12
CAACGCA	48445	0.0	36.192284	13
AGTGGTA	48285	0.0	36.166203	5
GTGGTAT	48525	0.0	35.997013	6
GGTATCA	48580	0.0	35.985283	8
GTATCAA	48815	0.0	35.76872	9
TGGTATC	48865	0.0	35.712883	7
AACGCAG	49240	0.0	35.64612	14
ATCAACG	49170	0.0	35.6204	11
ACGCAGA	49390	0.0	35.47125	15
TATCAAC	50015	0.0	34.92462	10
CGCAGAG	50440	0.0	34.802734	16
AGCAGTG	51325	0.0	34.16142	2
CAGTGGT	51285	0.0	34.041435	4
GCAGTGG	51855	0.0	33.893837	3
AGAGTAC	44710	0.0	33.060837	19
CAGAGTA	46400	0.0	32.033943	18
ACATGGG	36805	0.0	31.790926	24
GCAGAGT	48015	0.0	31.166927	17
>>END_MODULE