FastQCFastQC Report
Thu 26 May 2016
SRR805424_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805424_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16628135
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2981541.793069397139246No Hit
AAGCAGTGGTATCAACGCAGAGAACTTTTTTTTTTTTTTTTTTTTTTTTTTTT519800.3126027061964556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1347200.044.2369351
CAACGCA1396600.042.76979413
TCAACGC1395250.042.76570512
GTGGTAT1390250.042.579756
GGTATCA1393100.042.5769848
AGTGGTA1391700.042.5556535
AACGCAG1405650.042.53288714
ACGCAGA1403900.042.52564215
TGGTATC1392700.042.491357
ATCAACG1404450.042.4738511
GTATCAA1397950.042.4443979
TATCAAC1406200.042.2187810
CGCAGAG1414700.042.1926916
AGCAGTG1420250.042.012922
GCAGTGG1423450.041.892063
CAGTGGT1424350.041.6181074
AGAGTAC1215800.041.14336419
CAGAGTA1237450.040.53747618
GCAGAGT1247850.040.42561317
GAGTACT510550.040.38566620