##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805424_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16628135
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.87276703009688	35.0	33.0	35.0	32.0	36.0
2	35.101507234575614	35.0	35.0	37.0	33.0	37.0
3	37.6423275971719	39.0	37.0	39.0	35.0	39.0
4	37.696439378198455	39.0	37.0	39.0	35.0	39.0
5	37.68538895071516	39.0	38.0	39.0	35.0	39.0
6	37.74392293543443	39.0	38.0	39.0	35.0	39.0
7	37.65570769060992	39.0	37.0	39.0	35.0	39.0
8	38.686134133503245	40.0	39.0	40.0	36.0	40.0
9	38.628668398470424	40.0	39.0	40.0	36.0	40.0
10	38.371238746858864	40.0	38.0	40.0	35.0	40.0
11	38.442800891380784	40.0	38.0	40.0	36.0	40.0
12	38.505621827102075	40.0	39.0	40.0	36.0	40.0
13	38.65131531587878	40.0	39.0	40.0	36.0	40.0
14	38.67333059299795	40.0	39.0	40.0	36.0	40.0
15	38.57596020239191	40.0	39.0	40.0	36.0	40.0
16	38.572288173027225	40.0	39.0	40.0	36.0	40.0
17	38.528021633213825	40.0	39.0	40.0	35.0	40.0
18	38.49308398085534	40.0	39.0	40.0	35.0	40.0
19	38.46362186739523	40.0	39.0	40.0	35.0	40.0
20	38.34820471447941	40.0	39.0	40.0	35.0	40.0
21	38.29841368259279	40.0	38.0	40.0	35.0	40.0
22	38.29591237983093	40.0	38.0	40.0	35.0	40.0
23	32.952594382953954	40.0	35.0	40.0	3.0	40.0
24	34.898554588352816	40.0	36.0	40.0	16.0	40.0
25	36.61122657471809	40.0	35.0	40.0	30.0	40.0
26	37.388976755360716	40.0	37.0	40.0	33.0	40.0
27	37.72024024341876	40.0	38.0	40.0	33.0	40.0
28	37.80667152389609	40.0	38.0	40.0	34.0	40.0
29	37.8175171178247	40.0	38.0	40.0	34.0	40.0
30	37.722854427150125	40.0	38.0	40.0	33.0	40.0
31	37.66451980333333	40.0	38.0	40.0	33.0	40.0
32	37.7415396254601	40.0	38.0	40.0	33.0	40.0
33	37.793643664788625	40.0	38.0	40.0	33.0	40.0
34	37.7651144280462	40.0	38.0	40.0	33.0	40.0
35	37.7427131785976	40.0	38.0	40.0	33.0	40.0
36	37.67421433612368	40.0	38.0	40.0	33.0	40.0
37	37.21531590884967	40.0	37.0	40.0	32.0	40.0
38	37.27012590407764	40.0	37.0	40.0	32.0	40.0
39	37.28896157025427	40.0	37.0	40.0	33.0	40.0
40	37.238506723694506	40.0	37.0	40.0	32.0	40.0
41	37.12202005817249	40.0	36.0	40.0	32.0	40.0
42	37.08267319215294	40.0	36.0	40.0	32.0	40.0
43	37.14444710726729	40.0	36.0	40.0	32.0	40.0
44	36.95815074871596	40.0	36.0	40.0	31.0	40.0
45	36.771004986428125	39.0	36.0	40.0	31.0	40.0
46	36.70361330359658	39.0	35.0	40.0	31.0	40.0
47	36.63556213610246	39.0	35.0	40.0	31.0	40.0
48	36.58743364785047	39.0	35.0	40.0	31.0	40.0
49	36.61302930244432	39.0	35.0	40.0	31.0	40.0
50	36.59141731769678	39.0	35.0	40.0	31.0	40.0
51	36.51991813874496	39.0	35.0	40.0	31.0	40.0
52	36.4030880793306	39.0	35.0	40.0	30.0	40.0
53	35.975013914669326	38.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	8.0
12	15.0
13	68.0
14	193.0
15	589.0
16	1440.0
17	2543.0
18	4578.0
19	7537.0
20	11912.0
21	17904.0
22	26017.0
23	36528.0
24	49725.0
25	65896.0
26	86063.0
27	110472.0
28	140183.0
29	174879.0
30	216604.0
31	268711.0
32	334880.0
33	423448.0
34	557762.0
35	774937.0
36	1177107.0
37	2159514.0
38	3301854.0
39	6676767.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.865539941791429	45.16160711949957	21.750767599613546	21.222085339095457
2	26.470725670678043	41.811724525931496	17.64038480563214	14.077164997758318
3	32.86819598229146	25.21313424506116	21.963882299488187	19.954787473159197
4	20.894670388471106	25.956037763705915	21.973919504502458	31.17537234332052
5	19.710268168979862	34.02227610011585	25.5147014382551	20.752754292649175
6	28.697854569980336	24.193813677841803	24.34909266733762	22.759239084840242
7	22.74774651516842	24.647292074547146	31.501434165647563	21.103527244636876
8	27.995472733412374	25.374006164852524	25.39652823362331	21.233992868111788
9	28.66566815821498	24.502621610902246	25.598439031196225	21.233271199686556
10	21.31149404307819	25.677744377225707	31.880707006528393	21.130054573167705
11	20.86780026743829	32.973932434395074	25.013839495529716	21.144427802636915
12	20.954466631405143	25.039338446554588	32.26779792201591	21.738397000024356
13	21.10732201777289	25.803080140977926	24.471475604449928	28.618122236799255
14	21.3411906987765	33.32051369561289	24.34214660874476	20.996148996865855
15	21.18212896395176	32.72538381484153	24.382638221303832	21.709848999902874
16	21.617409288534162	25.10835400362097	24.447323767818823	28.826912940026045
17	29.03572769886701	24.900134621230823	24.549999143018745	21.514138536883422
18	21.407818736136072	25.021753792593092	24.40293514576349	29.16749232550734
19	21.694838296658045	32.723615727199714	24.330028593104398	21.251517383037847
20	29.211694516552818	25.29845950853779	24.424579184616917	21.065266790292476
21	21.876079307751592	32.35685781959312	24.386384883211495	21.380677989443793
22	28.918372385117152	25.005143391005667	24.735167233126266	21.341316990750915
23	18.37741875441834	36.01301649282978	27.489071985523335	18.120492767228555
24	21.53213815018942	32.10032875003721	24.51960487450938	21.84792822526399
25	21.345815390601533	25.16471630763161	24.505213603329537	28.98425469843732
26	21.50915902474932	29.28676607448761	28.03797900365856	21.166095897104515
27	21.91897648172811	24.57609948439798	32.01343987163924	21.49148416223467
28	26.171413691312946	24.844157207046973	27.524656252790823	21.459772848849255
29	26.312932869500997	24.883434011090237	27.572996009474306	21.23063710993446
30	26.262656635876482	24.59907259593454	27.55499038226476	21.58328038592422
31	24.29577941242358	25.671640265128953	27.909774607916045	22.122805714531424
32	23.27741505586766	26.23959331578677	28.323645435883215	22.159346192462355
33	23.22257426945355	25.707266629721254	28.427144715868618	22.64301438495658
34	22.820376428264506	25.94069028186264	28.585965894551613	22.652967395321244
35	22.70973864477285	25.997401392278807	28.79745082656594	22.495409136382403
36	22.82242115546933	25.742423909837154	28.71777261851675	22.717382316176767
37	23.345636777666286	25.80776497183839	28.525917067668743	22.320681182826576
38	23.013031828283808	25.926305024586345	28.780599868836767	22.280063278293085
39	23.083069748952603	25.638311211690308	28.69705472080904	22.58156431854805
40	22.88388926358849	26.034020050955803	28.452379055137573	22.629711630318134
41	22.883811082842424	26.166169567422926	28.5608337916429	22.389185558091754
42	23.066128582670274	25.86009796047482	28.487638571613715	22.586134885241187
43	22.933491940016122	26.02430759673289	28.652341347962356	22.389859115288637
44	23.018648814193536	25.93077335491924	28.627215258957182	22.423362571930046
45	23.0931791208094	25.530211295494055	28.57628952374996	22.800320059946593
46	22.876504190036947	25.85827574770111	28.438697424575878	22.826522637686068
47	22.93388284374646	25.915606290182275	28.48936456193073	22.66114630414054
48	23.05289198097081	25.606924649096246	28.471292781782203	22.868890588150744
49	22.772229116494426	26.114468038658572	28.37738567794885	22.73591716689815
50	23.081373828153307	26.009778005771544	28.444771467154915	22.464076698920234
51	23.110847969420504	25.727798096419114	28.320151357924384	22.841202576236
52	23.131848520594765	26.098861958962928	27.965776077714068	22.803513442728242
53	23.234187117196246	26.166157539615835	27.841534844406784	22.758120498781132
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10878.0
1	6398.0
2	1918.0
3	1538.0
4	1158.0
5	1193.5
6	1229.0
7	1435.0
8	2084.5
9	2528.0
10	3587.5
11	4647.0
12	6730.0
13	8813.0
14	11468.5
15	14124.0
16	20088.0
17	26052.0
18	36037.0
19	46022.0
20	63091.5
21	80161.0
22	298011.5
23	515862.0
24	374567.5
25	245652.5
26	258032.0
27	285577.0
28	313122.0
29	341414.0
30	369706.0
31	397956.5
32	426207.0
33	469026.5
34	511846.0
35	564340.0
36	616834.0
37	670719.0
38	724604.0
39	774378.5
40	824153.0
41	871122.5
42	959568.5
43	1001045.0
44	1040391.0
45	1079737.0
46	1102239.5
47	1124742.0
48	1142653.0
49	1160564.0
50	1154586.0
51	1148608.0
52	1116661.0
53	1084714.0
54	1038182.0
55	991650.0
56	931475.5
57	871301.0
58	786300.0
59	614780.0
60	528261.0
61	456740.5
62	385220.0
63	324216.5
64	263213.0
65	213877.0
66	164541.0
67	131302.5
68	98064.0
69	76190.5
70	54317.0
71	41169.0
72	28021.0
73	21354.5
74	14688.0
75	11252.0
76	6027.5
77	4239.0
78	3306.0
79	2373.0
80	1909.5
81	1446.0
82	1124.5
83	803.0
84	559.5
85	316.0
86	216.5
87	117.0
88	90.5
89	64.0
90	53.0
91	42.0
92	25.5
93	10.0
94	11.0
95	8.0
96	5.0
97	4.5
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.6628135E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.30195229772359
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.56555724848263	40.49055332760281
2	14.37701572761742	15.614000444494062
3	4.5411048632947395	7.3977257899677475
4	2.0359907432312334	4.422330888701969
5	1.0765954176895371	2.9230616507662526
6	0.7209194728608371	2.3488400895473527
7	0.4994680418969217	1.8985462849726835
8	0.36706353379739515	1.5945813202000003
9	0.2834441287221326	1.3852412601245165
>10	1.426966898264625	13.39230467548983
>50	0.07690525044051867	2.839759777146124
>100	0.02777060104909632	2.4442624112074913
>500	7.960703611105934E-4	0.2840162621032788
>1k	2.680306933819926E-4	0.24451373147390057
>5k	4.465719938091555E-5	0.16219432649796003
>10k+	8.931439876183097E-5	2.5580677597039756
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	298154	1.793069397139246	No Hit
AAGCAGTGGTATCAACGCAGAGAACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51980	0.3126027061964556	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.209732480521718E-5	0.0	0.0	0.0	0.0
2	4.811122834881964E-5	0.0	0.0	0.0	0.0
3	6.0139035436024545E-5	0.0	0.0	6.013903543602455E-6	0.0
4	6.0139035436024545E-5	0.0	0.0	6.013903543602455E-6	0.0
5	6.0139035436024545E-5	6.013903543602455E-6	0.0	1.8041710630807365E-5	6.013903543602455E-6
6	6.0139035436024545E-5	6.013903543602455E-6	0.0	1.8041710630807365E-5	6.013903543602455E-6
7	6.0139035436024545E-5	6.013903543602455E-6	0.0	1.8041710630807365E-5	1.202780708720491E-5
8	6.0139035436024545E-5	6.013903543602455E-6	0.0	2.405561417440982E-5	1.202780708720491E-5
9	6.0139035436024545E-5	1.202780708720491E-5	0.0	2.405561417440982E-5	1.8041710630807365E-5
10	6.0139035436024545E-5	1.8041710630807365E-5	0.0	2.405561417440982E-5	3.0069517718012273E-5
11	6.6152938979627E-5	1.8041710630807365E-5	0.0	3.0069517718012273E-5	4.811122834881964E-5
12	8.419464961043436E-5	1.8041710630807365E-5	0.0	7.216684252322946E-5	4.811122834881964E-5
13	8.419464961043436E-5	2.405561417440982E-5	0.0	7.216684252322946E-5	5.412513189242209E-5
14	1.0825026378484418E-4	2.405561417440982E-5	0.0	1.2027807087204909E-4	5.412513189242209E-5
15	1.0825026378484418E-4	2.405561417440982E-5	0.0	1.2027807087204909E-4	5.412513189242209E-5
16	1.0825026378484418E-4	2.405561417440982E-5	0.0	1.32305877959254E-4	5.412513189242209E-5
17	1.2027807087204909E-4	2.405561417440982E-5	0.0	1.32305877959254E-4	5.412513189242209E-5
18	1.2027807087204909E-4	2.405561417440982E-5	0.0	1.32305877959254E-4	5.412513189242209E-5
19	1.32305877959254E-4	2.405561417440982E-5	0.0	1.32305877959254E-4	7.216684252322946E-5
20	1.32305877959254E-4	3.0069517718012273E-5	0.0	1.32305877959254E-4	7.81807460668319E-5
21	1.563614921336638E-4	3.0069517718012273E-5	0.0	1.32305877959254E-4	7.81807460668319E-5
22	1.6237539567726626E-4	3.608342126161473E-5	0.0	1.32305877959254E-4	8.419464961043436E-5
23	1.7440320276447118E-4	3.608342126161473E-5	0.0	1.3831978150285646E-4	8.419464961043436E-5
24	2.104866240260859E-4	3.608342126161473E-5	0.0	1.4433368504645892E-4	8.419464961043436E-5
25	2.2852833465689327E-4	3.608342126161473E-5	0.0	1.563614921336638E-4	8.419464961043436E-5
26	2.525839488313031E-4	3.608342126161473E-5	0.0	1.6237539567726626E-4	9.020855315403682E-5
27	2.585978523749055E-4	3.608342126161473E-5	0.0	1.6838929922086872E-4	9.622245669763928E-5
28	2.7062565946211044E-4	3.608342126161473E-5	0.0	1.7440320276447118E-4	1.1426416732844663E-4
29	3.067090807237252E-4	3.608342126161473E-5	0.0	1.8041710630807364E-4	1.1426416732844663E-4
30	3.427925019853399E-4	3.608342126161473E-5	0.0	1.8041710630807364E-4	1.2027807087204909E-4
31	3.848898267905571E-4	4.209732480521718E-5	0.0	1.864310098516761E-4	1.2027807087204909E-4
32	4.63070572857389E-4	4.209732480521718E-5	0.0	1.864310098516761E-4	1.2629197441565155E-4
33	6.013903543602454E-4	4.209732480521718E-5	0.0	1.864310098516761E-4	1.32305877959254E-4
34	6.675432933398724E-4	4.811122834881964E-5	0.0	1.864310098516761E-4	1.32305877959254E-4
35	7.577518464939092E-4	4.811122834881964E-5	0.0	2.104866240260859E-4	1.5034758859006135E-4
36	7.998491712991264E-4	4.811122834881964E-5	0.0	2.1650052756968835E-4	1.5034758859006135E-4
37	8.479603996479461E-4	4.811122834881964E-5	0.0	2.1650052756968835E-4	1.5034758859006135E-4
38	9.26141145714778E-4	4.811122834881964E-5	0.0	2.1650052756968835E-4	1.5034758859006135E-4
39	0.001058447023674032	4.811122834881964E-5	0.0	2.1650052756968835E-4	1.563614921336638E-4
40	0.0011486555768280688	4.811122834881964E-5	0.0	2.225144311132908E-4	1.563614921336638E-4
41	0.0012629197441565154	4.811122834881964E-5	0.0	2.3454223820049572E-4	1.7440320276447118E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	134720	0.0	44.236935	1
CAACGCA	139660	0.0	42.769794	13
TCAACGC	139525	0.0	42.765705	12
GTGGTAT	139025	0.0	42.57975	6
GGTATCA	139310	0.0	42.576984	8
AGTGGTA	139170	0.0	42.555653	5
AACGCAG	140565	0.0	42.532887	14
ACGCAGA	140390	0.0	42.525642	15
TGGTATC	139270	0.0	42.49135	7
ATCAACG	140445	0.0	42.47385	11
GTATCAA	139795	0.0	42.444397	9
TATCAAC	140620	0.0	42.21878	10
CGCAGAG	141470	0.0	42.19269	16
AGCAGTG	142025	0.0	42.01292	2
GCAGTGG	142345	0.0	41.89206	3
CAGTGGT	142435	0.0	41.618107	4
AGAGTAC	121580	0.0	41.143364	19
CAGAGTA	123745	0.0	40.537476	18
GCAGAGT	124785	0.0	40.425613	17
GAGTACT	51055	0.0	40.385666	20
>>END_MODULE