FastQCFastQC Report
Thu 26 May 2016
SRR805423_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805423_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16940583
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT183440.10828434889165266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG33000.029.05454615
GCTCGTA36200.026.94060910
CTCGTAT35950.026.86648211
TCGTATG37500.025.69333312
AAGCAGT161900.024.6321811
CGTATGC39700.024.15113313
ATGCCGT41400.022.8188416
TGCCGTC46250.020.78162217
TCAACGC204250.020.6408812
CAACGCA204500.020.59266513
AACGCAG211350.019.99195714
ACGCAGA212000.019.96391715
GAGCTCG49050.019.9306838
GCCGTCT48050.019.80749318
ATCAACG215300.019.59242811
ATCGGAA50850.019.5486721
GGTATCA218100.019.2870248
GTATCAA218750.019.2297139
CCGTCTT50350.019.13604719
AGTGGTA220350.019.1220785
Produced by FastQC (version 0.11.5)