FastQCFastQC Report
Thu 26 May 2016
SRR805422_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805422_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25609748
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6750792.6360235953903177No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC427760.1670301480514373No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA282300.11023146342556747No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG279770.10924355835129655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2891400.045.1298521
TCAACGC2931950.044.1025312
CAACGCA2934750.044.05804413
AGTGGTA2939450.044.011585
GTATCAA2943300.043.997939
GTGGTAT2942450.043.969916
GGTATCA2948600.043.9260188
ATCAACG2954100.043.89275711
AACGCAG2948300.043.86831314
ACGCAGA2950650.043.81744815
TGGTATC2953700.043.8040287
TATCAAC2956300.043.7106510
AGCAGTG2985600.043.6673742
CGCAGAG2970900.043.5646616
GCAGTGG2990550.043.4709323
AGAGTAC2937650.043.4313819
CAGTGGT3000850.043.1447454
CAGAGTA2969100.043.01407618
GAGTACT1084300.042.96449320
GTACTTT1078300.042.8003822