##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805422_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25609748
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.973275644883344	37.0	35.0	37.0	35.0	37.0
2	36.07385914144879	37.0	35.0	37.0	35.0	37.0
3	37.806767212234966	39.0	38.0	39.0	35.0	39.0
4	37.77493726217064	39.0	38.0	39.0	35.0	39.0
5	37.70032543857909	39.0	37.0	39.0	35.0	39.0
6	37.71262977675532	39.0	37.0	39.0	35.0	39.0
7	37.612003132557184	39.0	37.0	39.0	35.0	39.0
8	38.56226613397367	40.0	39.0	40.0	36.0	40.0
9	38.54756579408747	40.0	39.0	40.0	36.0	40.0
10	38.24473473928756	40.0	38.0	40.0	34.0	40.0
11	38.43038041608219	40.0	38.0	40.0	35.0	40.0
12	38.57989387478549	40.0	38.0	40.0	36.0	40.0
13	38.663428745960324	40.0	39.0	40.0	36.0	40.0
14	38.6714601018331	40.0	39.0	40.0	36.0	40.0
15	38.61127020851591	40.0	39.0	40.0	36.0	40.0
16	38.44552523515655	40.0	39.0	40.0	35.0	40.0
17	38.48083999108464	40.0	39.0	40.0	35.0	40.0
18	38.416973607081175	40.0	38.0	40.0	35.0	40.0
19	38.43329325224129	40.0	38.0	40.0	35.0	40.0
20	38.37199659285987	40.0	38.0	40.0	35.0	40.0
21	38.278595869041744	40.0	38.0	40.0	34.0	40.0
22	38.29582352782229	40.0	38.0	40.0	35.0	40.0
23	37.76151401411681	40.0	37.0	40.0	34.0	40.0
24	38.01842700677882	40.0	38.0	40.0	34.0	40.0
25	38.12256563399218	40.0	38.0	40.0	34.0	40.0
26	38.03042364961967	40.0	38.0	40.0	34.0	40.0
27	38.11458035432445	40.0	38.0	40.0	34.0	40.0
28	38.095934797952715	40.0	38.0	40.0	34.0	40.0
29	38.004328625178196	40.0	38.0	40.0	34.0	40.0
30	37.90407387062145	40.0	38.0	40.0	34.0	40.0
31	37.837733897264435	40.0	38.0	40.0	33.0	40.0
32	37.76214291526804	40.0	38.0	40.0	33.0	40.0
33	37.66891458674252	40.0	38.0	40.0	33.0	40.0
34	37.65042014470428	40.0	38.0	40.0	33.0	40.0
35	37.581204586628495	40.0	37.0	40.0	33.0	40.0
36	37.512378684866405	40.0	37.0	40.0	33.0	40.0
37	37.38329686024244	40.0	37.0	40.0	33.0	40.0
38	37.221707609149455	40.0	36.0	40.0	33.0	40.0
39	37.17135283798966	40.0	36.0	40.0	32.0	40.0
40	37.1111046465588	40.0	36.0	40.0	32.0	40.0
41	37.05565638521707	39.0	36.0	40.0	32.0	40.0
42	36.96696285336349	39.0	36.0	40.0	32.0	40.0
43	36.84349443813348	39.0	35.0	40.0	31.0	40.0
44	36.74629957311568	39.0	35.0	40.0	31.0	40.0
45	36.59554029973274	39.0	35.0	40.0	31.0	40.0
46	36.49615962640476	39.0	35.0	40.0	31.0	40.0
47	36.25286113709514	39.0	35.0	40.0	30.0	40.0
48	36.21203961085443	38.0	35.0	40.0	30.0	40.0
49	36.03760439189015	38.0	35.0	40.0	30.0	40.0
50	35.936077348359696	38.0	35.0	40.0	30.0	40.0
51	35.73927662232366	38.0	35.0	40.0	29.0	40.0
52	35.49256603383993	38.0	34.0	40.0	28.0	40.0
53	35.228924704764765	38.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	10.0
13	33.0
14	93.0
15	336.0
16	807.0
17	1756.0
18	3468.0
19	6670.0
20	11528.0
21	19400.0
22	30418.0
23	45885.0
24	66049.0
25	92095.0
26	122932.0
27	161261.0
28	206038.0
29	261314.0
30	327275.0
31	408267.0
32	509416.0
33	648427.0
34	848088.0
35	1160410.0
36	1688700.0
37	2778125.0
38	4827405.0
39	1.1383537E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.676891627360018	36.85662193942713	29.722104254989155	20.744382178223695
2	26.437784549851877	43.24923462737704	12.32503732563085	17.98794349714023
3	35.9358631721015	25.72896461144405	17.649369294848196	20.685802921606257
4	22.11685956456893	23.0197188976635	19.632758588643668	35.230662949123904
5	20.460138850253426	34.35053324226384	23.175163613480304	22.014164294002427
6	32.63972765370436	23.245492302384232	21.48778269899415	22.626997344917253
7	22.346401065719192	22.470935676524423	33.41677161368398	21.765891644072404
8	32.187021129610486	24.561151480287897	22.508558069372647	20.743269320728967
9	32.13856692381354	23.984023583519836	22.831782647763657	21.045626844902966
10	20.975110727368342	24.23346375762854	33.675122457276814	21.1163030577263
11	20.914208136682955	34.93067171141239	23.051370907671565	21.103749244233093
12	20.692144256944662	23.93046585229968	34.013317897544326	21.364071993211333
13	20.695701496164666	24.115446196502987	23.318245849197737	31.870606458134613
14	21.250474623959594	34.999516590323346	22.904629908892502	20.845378876824558
15	21.016403597567614	34.64873609845751	22.963732403770624	21.37112790020425
16	21.309541975969463	23.573008215465453	23.30529374986431	31.812156058700776
17	32.07990566717017	23.565991356103932	23.153402368504366	21.200700608221528
18	21.148665734625737	23.82162838931488	23.14398017504897	31.885725701010415
19	21.31871816934708	34.480304140439024	23.204316575079144	20.996661115134753
20	32.22268333136273	23.77490789835183	23.17221161254691	20.83019715773853
21	21.272716154801678	34.45813289533345	23.205788670782702	21.063362279082167
22	31.812464534988788	23.45850884592851	23.59648755622273	21.13253906285997
23	21.234254237878485	23.826946676710758	33.882195951322906	21.056603134087847
24	21.20827194394884	34.20885281651346	23.220654104054443	21.362221135483253
25	21.087435143836636	23.65993995723816	23.392830729923624	31.85979416900159
26	21.41837162942798	30.360497885414567	27.2279172758748	20.99321320928265
27	21.56361319916151	23.5076307662223	33.86043861111011	21.06831742350608
28	28.180706815233016	23.50172676435551	27.208170888678794	21.109395531732684
29	28.31890809702618	23.619299182483168	26.98020300707371	21.08158971341694
30	28.146817375946064	23.565265070160002	27.071379226378955	21.216538327514975
31	25.132789280081948	24.800130793946117	27.903078156020904	22.164001769951035
32	23.762545418252458	25.82672426140234	28.126614131462752	22.284116188882454
33	23.484530187489543	25.2010601588114	28.570242081257497	22.744167572441558
34	23.038309474970234	25.010300765161762	29.01638860327716	22.935001156590843
35	23.065213293000774	25.226886262215466	28.81204844342865	22.89585200135511
36	22.952068876273206	25.273220181627714	28.94514229503547	22.82956864706361
37	23.17936513861831	25.01199152760113	29.0242098438454	22.78443348993516
38	23.472882279044683	25.149411856766417	28.733297180433016	22.644408683755888
39	23.42324883477963	25.04920392031972	28.786355101971328	22.741192142929325
40	23.052542336613385	25.212977495912885	28.724741844394565	23.009738323079166
41	23.290666507144074	25.587100661826113	28.466406619854283	22.655826211175526
42	23.373802819145272	25.417485560576385	28.613467809210775	22.59524381106757
43	23.245293159464122	25.21726102107682	28.962780110136187	22.574665709322872
44	23.36831272217126	25.17410948362319	28.590656182950337	22.866921611255215
45	23.27414154953809	24.939655790443545	28.713410221763997	23.07279243825437
46	23.163753895586947	25.01039838424025	28.632737815303766	23.193109904869036
47	23.336203854875887	25.385720312437282	28.309595236938684	22.96848059574815
48	23.345505000673963	25.086896598904445	28.552822932892585	23.014775467529006
49	23.02463108969288	25.42886794512777	28.50480215580411	23.041698809375244
50	23.496572477011487	25.3058952395783	28.27422979718504	22.923302486225168
51	23.33969471312252	25.25681627167905	28.32368752710882	23.079801488089615
52	23.422811501308015	25.281275707984317	28.205892537482214	23.09002025322545
53	23.751697986251173	25.469832815223327	27.643059978567535	23.135409219957964
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9345.0
1	5293.0
2	1241.0
3	983.5
4	726.0
5	751.0
6	776.0
7	930.0
8	1408.5
9	1733.0
10	2659.5
11	3586.0
12	5528.5
13	7471.0
14	9823.0
15	12175.0
16	18255.5
17	24336.0
18	33959.5
19	43583.0
20	64649.5
21	85716.0
22	487140.0
23	888564.0
24	596680.5
25	311124.0
26	317451.0
27	347121.5
28	376792.0
29	413295.0
30	449798.0
31	486556.0
32	523314.0
33	585176.5
34	647039.0
35	726615.5
36	806192.0
37	888686.5
38	971181.0
39	1055444.0
40	1139707.0
41	1222998.0
42	1385967.0
43	1465645.0
44	1548669.0
45	1631693.0
46	1684051.5
47	1736410.0
48	1794529.5
49	1852649.0
50	1852989.0
51	1853329.0
52	1825079.5
53	1796830.0
54	1751817.0
55	1706804.0
56	1619027.5
57	1531251.0
58	1388434.0
59	1097045.0
60	948473.0
61	826981.5
62	705490.0
63	598885.5
64	492281.0
65	400786.0
66	309291.0
67	248758.0
68	188225.0
69	147045.5
70	105866.0
71	81140.0
72	56414.0
73	42291.5
74	28169.0
75	21760.0
76	11594.5
77	7838.0
78	6004.0
79	4170.0
80	3434.5
81	2699.0
82	2057.5
83	1416.0
84	953.0
85	490.0
86	334.0
87	178.0
88	155.5
89	133.0
90	108.0
91	83.0
92	59.5
93	23.0
94	10.0
95	7.5
96	5.0
97	5.5
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.5609748E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.29055386710619
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.60287293859918	32.68246092936678
2	15.91332101476449	14.73272887277017
3	5.461303743385066	7.584203253533853
4	2.420325396373966	4.481528125470968
5	1.3752132451743522	3.182969140225063
6	0.8057804507569412	2.2380014016495178
7	0.5539184606985318	1.794883464006495
8	0.3905658351264248	1.4463607063656865
9	0.3026056449823936	1.2607004618593116
>10	1.993415479700027	17.398147194451038
>50	0.12461411642782307	3.9354164564891763
>100	0.053088969642154586	4.228401838481794
>500	0.0020538250476136687	0.6225320685784762
>1k	7.459234655927161E-4	0.6248680596655343
>5k	9.62257205872685E-5	0.29594242658213626
>10k+	7.873013502412061E-5	3.490855600504071
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	675079	2.6360235953903177	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	42776	0.1670301480514373	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	28230	0.11023146342556747	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	27977	0.10924355835129655	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.247621023057313E-5	0.0	0.0	0.0	7.809526278821642E-6
2	7.809526278821643E-5	0.0	0.0	0.0	7.809526278821642E-6
3	8.200002592762725E-5	3.904763139410821E-6	0.0	0.0	1.1714289418232464E-5
4	9.761907848527053E-5	7.809526278821642E-6	0.0	0.0	1.9523815697054107E-5
5	1.0152384162468135E-4	7.809526278821642E-6	0.0	0.0	3.1238105115286566E-5
6	1.0542860476409218E-4	7.809526278821642E-6	0.0	1.1714289418232464E-5	3.1238105115286566E-5
7	1.0542860476409218E-4	7.809526278821642E-6	0.0	1.1714289418232464E-5	3.1238105115286566E-5
8	1.0542860476409218E-4	7.809526278821642E-6	0.0	1.1714289418232464E-5	3.1238105115286566E-5
9	1.09333367903503E-4	1.1714289418232464E-5	0.0	1.1714289418232464E-5	4.6857157672929857E-5
10	1.09333367903503E-4	1.1714289418232464E-5	0.0	1.1714289418232464E-5	5.0761920812340674E-5
11	1.1323813104291381E-4	1.1714289418232464E-5	0.0	1.5619052557643283E-5	6.638097336998396E-5
12	1.1714289418232463E-4	1.1714289418232464E-5	0.0	2.3428578836464928E-5	7.41904996488056E-5
13	1.2104765732173546E-4	1.1714289418232464E-5	0.0	2.3428578836464928E-5	7.809526278821643E-5
14	1.2495242046114627E-4	1.1714289418232464E-5	0.0	2.3428578836464928E-5	1.0152384162468135E-4
15	1.3666670987937875E-4	1.1714289418232464E-5	0.0	2.3428578836464928E-5	1.0152384162468135E-4
16	1.4447623615820038E-4	1.1714289418232464E-5	0.0	2.733334197587575E-5	1.2104765732173546E-4
17	1.483809992976112E-4	1.1714289418232464E-5	0.0	2.733334197587575E-5	1.288571836005571E-4
18	1.6009528871584368E-4	1.1714289418232464E-5	0.0	3.1238105115286566E-5	1.4057147301878956E-4
19	1.7961910441289776E-4	1.1714289418232464E-5	0.0	3.9047631394108215E-5	1.483809992976112E-4
20	1.9133339383113024E-4	1.1714289418232464E-5	0.0	3.9047631394108215E-5	1.7961910441289776E-4
21	2.069524463887735E-4	1.1714289418232464E-5	0.0	4.295239453351903E-5	1.8742863069171943E-4
22	2.2647626208582763E-4	1.1714289418232464E-5	0.0	4.295239453351903E-5	2.1085720952818436E-4
23	2.381905515040601E-4	1.1714289418232464E-5	0.0	4.295239453351903E-5	2.2647626208582763E-4
24	2.928572354558116E-4	1.1714289418232464E-5	0.0	4.6857157672929857E-5	2.381905515040601E-4
25	3.0457152487404403E-4	1.5619052557643283E-5	0.0	4.6857157672929857E-5	2.381905515040601E-4
26	3.475239194075631E-4	1.5619052557643283E-5	0.0	4.6857157672929857E-5	2.4209531464347092E-4
27	3.9438107708049294E-4	1.9523815697054107E-5	0.0	5.0761920812340674E-5	2.460000777828817E-4
28	4.3342870847460117E-4	1.9523815697054107E-5	0.0	5.46666839517515E-5	2.6161913034052504E-4
29	4.685715767292985E-4	1.9523815697054107E-5	0.0	5.46666839517515E-5	2.6161913034052504E-4
30	5.310477869598717E-4	1.9523815697054107E-5	0.0	5.46666839517515E-5	2.8504770917698994E-4
31	6.13047812887499E-4	2.3428578836464928E-5	0.0	5.46666839517515E-5	2.8895247231640076E-4
32	7.419049964880561E-4	2.3428578836464928E-5	0.0	5.46666839517515E-5	2.967619985952224E-4
33	8.434288381127374E-4	2.3428578836464928E-5	0.0	5.46666839517515E-5	3.0457152487404403E-4
34	9.293336271797754E-4	2.3428578836464928E-5	0.0	5.46666839517515E-5	3.0457152487404403E-4
35	0.001026952705665046	2.3428578836464928E-5	0.0	5.46666839517515E-5	3.0457152487404403E-4
36	0.0011050479684532623	2.3428578836464928E-5	0.0	6.247621023057313E-5	3.1628581429227654E-4
37	0.0012026670469385329	2.3428578836464928E-5	0.0	6.247621023057313E-5	3.2409534057109817E-4
38	0.0012729527834479278	2.733334197587575E-5	0.0	7.028573650939478E-5	3.28000103710509E-4
39	0.0013705718619331983	2.733334197587575E-5	0.0	7.41904996488056E-5	3.28000103710509E-4
40	0.0015189528612308094	3.514286825469739E-5	0.0	7.41904996488056E-5	3.28000103710509E-4
41	0.0016673338605284207	3.514286825469739E-5	0.0	8.200002592762725E-5	3.4361915626815226E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	289140	0.0	45.129852	1
TCAACGC	293195	0.0	44.10253	12
CAACGCA	293475	0.0	44.058044	13
AGTGGTA	293945	0.0	44.01158	5
GTATCAA	294330	0.0	43.99793	9
GTGGTAT	294245	0.0	43.96991	6
GGTATCA	294860	0.0	43.926018	8
ATCAACG	295410	0.0	43.892757	11
AACGCAG	294830	0.0	43.868313	14
ACGCAGA	295065	0.0	43.817448	15
TGGTATC	295370	0.0	43.804028	7
TATCAAC	295630	0.0	43.71065	10
AGCAGTG	298560	0.0	43.667374	2
CGCAGAG	297090	0.0	43.56466	16
GCAGTGG	299055	0.0	43.470932	3
AGAGTAC	293765	0.0	43.43138	19
CAGTGGT	300085	0.0	43.144745	4
CAGAGTA	296910	0.0	43.014076	18
GAGTACT	108430	0.0	42.964493	20
GTACTTT	107830	0.0	42.80038	22
>>END_MODULE