FastQCFastQC Report
Thu 26 May 2016
SRR805421_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805421_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11430871
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT202780.1773968055452642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT162450.032.852261
TCAACGC218650.024.56940512
AGCAGTG220100.024.3861892
CAACGCA220750.024.33567413
AGTGGTA219750.024.2753125
AACGCAG224900.023.87616714
ACGCAGA224950.023.78728715
GGTATCA224500.023.7721618
GTGGTAT224750.023.7561746
ATCAACG227150.023.69139311
GTATCAA226100.023.5831499
TGGTATC227000.023.4689437
GCAGTGG232650.023.2525253
CGCAGAG233900.022.89717916
TATCAAC234950.022.7448410
CAGTGGT242100.022.170184
AGAGTAC214550.020.51526619
CAGAGTA229150.019.32096918
ACATGGG175100.019.23215124
GAGTACT80700.019.10285220
Produced by FastQC (version 0.11.5)