##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805421_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11430871
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.14227017346272	37.0	35.0	37.0	32.0	37.0
2	35.40282337190228	37.0	35.0	37.0	33.0	37.0
3	37.2661088555719	39.0	37.0	39.0	34.0	39.0
4	37.35295989255762	39.0	37.0	39.0	34.0	39.0
5	37.37231458565144	39.0	37.0	39.0	34.0	39.0
6	37.41077902112621	39.0	37.0	39.0	34.0	39.0
7	37.43160910485299	39.0	37.0	39.0	34.0	39.0
8	38.38174221369483	40.0	38.0	40.0	35.0	40.0
9	38.294935180355026	40.0	38.0	40.0	34.0	40.0
10	38.30783769670745	40.0	38.0	40.0	34.0	40.0
11	38.20272549659602	40.0	38.0	40.0	34.0	40.0
12	38.189390029858615	40.0	38.0	40.0	34.0	40.0
13	38.29069779546983	40.0	38.0	40.0	34.0	40.0
14	38.28971458080491	40.0	38.0	40.0	34.0	40.0
15	38.21733654417061	40.0	38.0	40.0	34.0	40.0
16	38.19813993176898	40.0	38.0	40.0	34.0	40.0
17	38.170148801434294	40.0	38.0	40.0	34.0	40.0
18	38.12567222567729	40.0	38.0	40.0	34.0	40.0
19	38.091866665278616	40.0	38.0	40.0	34.0	40.0
20	37.94577622300173	40.0	38.0	40.0	34.0	40.0
21	37.91488041462457	40.0	38.0	40.0	34.0	40.0
22	37.8737486408516	40.0	38.0	40.0	34.0	40.0
23	32.43224247741051	40.0	34.0	40.0	3.0	40.0
24	34.367382590530504	40.0	34.0	40.0	16.0	40.0
25	36.07907612639492	40.0	34.0	40.0	28.0	40.0
26	36.91181179456929	40.0	36.0	40.0	30.0	40.0
27	37.21901786836716	40.0	37.0	40.0	31.0	40.0
28	37.27353156203058	40.0	37.0	40.0	31.0	40.0
29	37.25919862099747	40.0	37.0	40.0	31.0	40.0
30	37.19188686496418	40.0	37.0	40.0	31.0	40.0
31	37.17331750135226	40.0	37.0	40.0	31.0	40.0
32	37.283718624766216	40.0	37.0	40.0	31.0	40.0
33	37.31980966279822	40.0	37.0	40.0	32.0	40.0
34	37.29083986688328	40.0	37.0	40.0	32.0	40.0
35	37.23931308471594	40.0	37.0	40.0	31.0	40.0
36	37.202973159263195	40.0	37.0	40.0	31.0	40.0
37	36.82185530743895	40.0	37.0	40.0	30.0	40.0
38	36.835847067122	40.0	37.0	40.0	30.0	40.0
39	36.920992022392696	40.0	37.0	40.0	31.0	40.0
40	36.804886259323546	40.0	36.0	40.0	30.0	40.0
41	36.762623163186774	40.0	36.0	40.0	30.0	40.0
42	36.73729788394952	40.0	36.0	40.0	30.0	40.0
43	36.792722706782364	40.0	36.0	40.0	30.0	40.0
44	36.48399513912807	39.0	36.0	40.0	30.0	40.0
45	36.35717216999475	39.0	35.0	40.0	30.0	40.0
46	36.30565299879598	39.0	35.0	40.0	30.0	40.0
47	36.22161810766651	39.0	35.0	40.0	29.0	40.0
48	36.16630185048891	39.0	35.0	40.0	29.0	40.0
49	36.239731775470126	39.0	35.0	40.0	29.0	40.0
50	36.26823975180894	39.0	35.0	40.0	29.0	40.0
51	36.27420045244146	39.0	35.0	40.0	29.0	40.0
52	36.23351650106103	39.0	35.0	40.0	29.0	40.0
53	35.800029324099626	38.0	35.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	10.0
11	43.0
12	132.0
13	317.0
14	763.0
15	1494.0
16	2702.0
17	4687.0
18	7524.0
19	11577.0
20	16885.0
21	23537.0
22	31862.0
23	42016.0
24	54318.0
25	69058.0
26	86983.0
27	106284.0
28	130791.0
29	157441.0
30	188714.0
31	225721.0
32	269093.0
33	324495.0
34	404319.0
35	529841.0
36	754540.0
37	1318810.0
38	2206378.0
39	4460533.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.287927927801828	28.85966432479205	23.04136753883409	24.81104020857203
2	25.706754979563673	23.95254919769456	28.503934652048823	21.83676117069294
3	21.92028936377639	25.298212183481034	26.910906439238097	25.870592013504485
4	22.341604589886458	28.145458032025733	26.157534277134264	23.355403100953552
5	25.44657358131327	27.627955909921475	24.79201278712707	22.133457721638187
6	24.75245324700104	25.551141290982986	27.042654929794942	22.653750532221036
7	23.84870759192366	26.30558073833569	27.02096804346755	22.824743626273097
8	23.672526791702925	25.54130826950982	26.996639188737237	23.789525750050018
9	23.42728738693666	25.617487941207628	27.565432240465316	23.3897924313904
10	22.80702844078986	26.337013163738792	27.670962256506964	23.18499613896439
11	22.739806966590734	27.069599508208952	26.951305810379626	23.23928771482068
12	22.776978237266434	26.029976193415184	27.488753919102056	23.70429165021633
13	23.021491538133883	26.453251025228084	26.23720449649025	24.288052940147782
14	23.219796636669244	27.164710370714534	26.29577396158175	23.31971903103447
15	23.158567706695315	26.738268676114007	26.30240512730832	23.800758489882355
16	23.547951857736827	26.05917781768336	26.053132784019695	24.33973754056012
17	24.45652654115334	25.860741495551824	26.03717599472516	23.645555968569674
18	23.390107368021212	26.08978790855045	26.037893350384234	24.482211373044102
19	23.80960296026436	26.879990159979933	25.946719195763823	23.363687683991884
20	24.47464414566484	25.98074110013139	26.129592399389338	23.415022354814433
21	23.671022094466817	26.70542778411199	26.149030988102307	23.474519133318886
22	24.40286483855867	25.842177730813336	26.224668268935936	23.530289161692053
23	19.786479962900465	37.64734988261175	22.81116635818915	19.755003796298638
24	23.372129735345627	26.61173413644507	26.2214401684701	23.7946959597392
25	23.615925680554	25.90643355173897	26.09462568512933	24.383015082577696
26	23.677443302439507	26.266379876039192	26.776795923950154	23.279380897571148
27	23.88196839943343	25.543801517837096	27.070885499451443	23.50334458327804
28	24.570384881432044	25.719483668392375	26.24094874310103	23.469182707074555
29	24.612087740295554	25.705136555210885	26.35213887025757	23.33063683423599
30	24.316064803810665	25.685986658409494	26.33400376926658	23.663944768513264
31	24.158325292971988	25.959911541298997	26.265505052064714	23.616258113664305
32	23.94882244756327	26.00596227531568	26.454283317517973	23.59093195960308
33	23.8552162822938	25.84607069749978	26.461894286095962	23.836818734110462
34	23.934930242848512	25.962378544907033	26.361718192778135	23.74097301946632
35	23.903480320965915	25.948984989857728	26.496965979232908	23.65056870994345
36	23.835585232306443	25.832257226942723	26.42809108772201	23.90406645302882
37	24.4189965926481	25.881326103671366	26.220696568091796	23.47898073558874
38	23.94408964986133	25.996680392946438	26.42068132865816	23.638548628534082
39	23.917031344330628	25.83914209162189	26.405231937268823	23.838594626778658
40	24.084525142484768	25.937104880284277	26.23402013722314	23.744349840007818
41	23.847841516188925	25.97768796446045	26.46568227390546	23.708788245445163
42	23.87724434997123	25.822861617456798	26.40741024896528	23.892483783606693
43	24.024450980157155	25.942380068850397	26.216628636610455	23.816540314381992
44	24.061079859968675	25.86715395528477	26.41091829310295	23.660847891643602
45	23.899141194052493	25.764510858358914	26.433243800931706	23.90310414665689
46	24.0008569775654	25.91771003277003	26.2293660736789	23.852066915985667
47	23.921641666676145	25.90813945848921	26.362068122367926	23.80815075246672
48	23.88166221104236	25.822730393860628	26.37024772652933	23.92535966856769
49	23.94674911474375	25.963629543190542	26.250755519854962	23.838865822210746
50	23.986404885506975	25.92079816139995	26.358805028943117	23.73399192414996
51	23.95442132099995	25.82537236226356	26.27769135002923	23.94251496670726
52	24.06708990067336	25.942511292446568	26.122838758306344	23.867560048573726
53	23.979983677534282	25.999173641273703	26.223522249529367	23.797320431662644
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3121.0
1	2065.0
2	1009.0
3	921.0
4	833.0
5	904.5
6	976.0
7	1092.5
8	1453.5
9	1698.0
10	2252.5
11	2807.0
12	4037.0
13	5267.0
14	6826.0
15	8385.0
16	11778.0
17	15171.0
18	20982.0
19	26793.0
20	36109.0
21	45425.0
22	74283.5
23	103142.0
24	103281.5
25	121679.5
26	139938.0
27	159920.5
28	179903.0
29	200641.5
30	221380.0
31	243113.0
32	264846.0
33	293888.0
34	322930.0
35	359713.0
36	396496.0
37	434944.0
38	473392.0
39	508295.0
40	543198.0
41	579724.5
42	650072.0
43	683893.0
44	712953.5
45	742014.0
46	766365.5
47	790717.0
48	803222.0
49	815727.0
50	821988.0
51	828249.0
52	815797.5
53	803346.0
54	776181.0
55	749016.0
56	703849.5
57	658683.0
58	605537.0
59	491545.5
60	430700.0
61	376995.5
62	323291.0
63	275991.0
64	228691.0
65	188073.5
66	147456.0
67	119290.5
68	91125.0
69	71493.5
70	51862.0
71	39386.5
72	26911.0
73	20248.5
74	13586.0
75	10381.0
76	5493.0
77	3810.0
78	2876.0
79	1942.0
80	1566.5
81	1191.0
82	966.0
83	741.0
84	542.5
85	344.0
86	269.0
87	194.0
88	147.5
89	101.0
90	81.0
91	61.0
92	49.5
93	27.5
94	17.0
95	11.0
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.1430871E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.37359484311588
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.13416394194053	53.694055563184776
2	10.495105682828067	13.722055734897701
3	2.987007378784746	5.858142305222145
4	1.357649126367752	3.5501761570510255
5	0.740175554245838	2.4193968398023076
6	0.46970999057557783	1.842397837059096
7	0.3215840188643401	1.4716172350080812
8	0.24988973077419196	1.3068952012069863
9	0.1928410648586703	1.134604227886717
>10	0.9951824813878666	11.344330684469508
>50	0.044886389207458	1.945900252332837
>100	0.011574789231301522	1.3292746687306525
>500	1.2184360859292752E-4	0.05686175028334233
>1k	8.100585892939246E-5	0.0929643229469514
>5k	1.3500733062766596E-5	0.05235532501155709
>10k+	1.3500733062766596E-5	0.17897189490633086
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20278	0.1773968055452642	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.4118135004760356E-4	0.0	0.0	0.0	1.7496479489620694E-5
2	3.4992958979241387E-4	0.0	0.0	0.0	1.7496479489620694E-5
3	3.4992958979241387E-4	0.0	0.0	0.0	2.624471923443104E-5
4	3.4992958979241387E-4	0.0	0.0	8.748239744810347E-6	4.3741198724051734E-5
5	3.4992958979241387E-4	8.748239744810347E-6	0.0	8.748239744810347E-6	4.3741198724051734E-5
6	3.761743090268449E-4	8.748239744810347E-6	0.0	8.748239744810347E-6	5.248943846886208E-5
7	3.761743090268449E-4	8.748239744810347E-6	0.0	1.7496479489620694E-5	5.248943846886208E-5
8	3.761743090268449E-4	8.748239744810347E-6	0.0	1.7496479489620694E-5	5.248943846886208E-5
9	3.761743090268449E-4	8.748239744810347E-6	0.0	1.7496479489620694E-5	6.123767821367243E-5
10	3.761743090268449E-4	8.748239744810347E-6	0.0	1.7496479489620694E-5	7.873415770329313E-5
11	3.761743090268449E-4	8.748239744810347E-6	0.0	1.7496479489620694E-5	7.873415770329313E-5
12	3.761743090268449E-4	2.624471923443104E-5	0.0	6.998591795848277E-5	9.623063719291382E-5
13	3.761743090268449E-4	2.624471923443104E-5	0.0	6.998591795848277E-5	1.1372711668253452E-4
14	3.761743090268449E-4	2.624471923443104E-5	0.0	1.4872007566177592E-4	1.2247535642734487E-4
15	3.761743090268449E-4	2.624471923443104E-5	0.0	1.4872007566177592E-4	1.3997183591696555E-4
16	3.761743090268449E-4	2.624471923443104E-5	0.0	1.4872007566177592E-4	1.4872007566177592E-4
17	3.849225487716553E-4	2.624471923443104E-5	0.0	1.4872007566177592E-4	1.4872007566177592E-4
18	3.849225487716553E-4	2.624471923443104E-5	0.0	1.4872007566177592E-4	1.4872007566177592E-4
19	4.1116726800608633E-4	2.624471923443104E-5	0.0	1.4872007566177592E-4	1.4872007566177592E-4
20	4.28663747495707E-4	3.499295897924139E-5	0.0	1.4872007566177592E-4	1.4872007566177592E-4
21	4.5490846673013806E-4	4.3741198724051734E-5	0.0	1.5746831540658626E-4	1.4872007566177592E-4
22	4.7240494621975874E-4	4.3741198724051734E-5	0.0	1.5746831540658626E-4	1.7496479489620694E-4
23	4.811531859645691E-4	5.248943846886208E-5	0.0	1.5746831540658626E-4	1.837130346410173E-4
24	5.073979051990001E-4	5.248943846886208E-5	0.0	1.5746831540658626E-4	1.837130346410173E-4
25	5.336426244334311E-4	5.248943846886208E-5	0.0	1.5746831540658626E-4	1.837130346410173E-4
26	5.686355834126726E-4	5.248943846886208E-5	0.0	1.5746831540658626E-4	1.9246127438582764E-4
27	6.03628542391914E-4	5.248943846886208E-5	0.0	1.5746831540658626E-4	2.0120951413063798E-4
28	6.561179808607761E-4	5.248943846886208E-5	0.0	1.5746831540658626E-4	2.0120951413063798E-4
29	7.086074193296382E-4	5.248943846886208E-5	0.0	1.5746831540658626E-4	2.0120951413063798E-4
30	7.436003783088795E-4	5.248943846886208E-5	0.0	1.5746831540658626E-4	2.187059936202587E-4
31	8.398310155017933E-4	5.248943846886208E-5	0.0	1.5746831540658626E-4	2.187059936202587E-4
32	9.010686937154658E-4	5.248943846886208E-5	0.0	1.5746831540658626E-4	2.187059936202587E-4
33	9.885510911635692E-4	5.248943846886208E-5	0.0	1.662165551513966E-4	2.3620247310987937E-4
34	0.0011022782078461038	5.248943846886208E-5	0.0	1.662165551513966E-4	2.4495071285468974E-4
35	0.0011635158860597763	5.248943846886208E-5	0.0	1.662165551513966E-4	2.711954320891208E-4
36	0.0012247535642734486	5.248943846886208E-5	0.0	1.662165551513966E-4	2.799436718339311E-4
37	0.0013384806809559832	6.123767821367243E-5	0.0	1.662165551513966E-4	2.8869191157874146E-4
38	0.001460956037383328	6.123767821367243E-5	0.0	1.662165551513966E-4	3.149366308131725E-4
39	0.0015659349143210522	6.123767821367243E-5	0.0	1.662165551513966E-4	3.149366308131725E-4
40	0.0016534173117691558	7.873415770329313E-5	0.0	1.662165551513966E-4	3.149366308131725E-4
41	0.0017671444284516901	7.873415770329313E-5	0.0	1.662165551513966E-4	3.4118135004760356E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	16245	0.0	32.85226	1
TCAACGC	21865	0.0	24.569405	12
AGCAGTG	22010	0.0	24.386189	2
CAACGCA	22075	0.0	24.335674	13
AGTGGTA	21975	0.0	24.275312	5
AACGCAG	22490	0.0	23.876167	14
ACGCAGA	22495	0.0	23.787287	15
GGTATCA	22450	0.0	23.772161	8
GTGGTAT	22475	0.0	23.756174	6
ATCAACG	22715	0.0	23.691393	11
GTATCAA	22610	0.0	23.583149	9
TGGTATC	22700	0.0	23.468943	7
GCAGTGG	23265	0.0	23.252525	3
CGCAGAG	23390	0.0	22.897179	16
TATCAAC	23495	0.0	22.74484	10
CAGTGGT	24210	0.0	22.17018	4
AGAGTAC	21455	0.0	20.515266	19
CAGAGTA	22915	0.0	19.320969	18
ACATGGG	17510	0.0	19.232151	24
GAGTACT	8070	0.0	19.102852	20
>>END_MODULE