FastQCFastQC Report
Thu 26 May 2016
SRR805420_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805420_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18589801
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3863332.078198685397439No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG297940.16027067745372853No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC218640.11761287815829766No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG210150.11304585777975784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1716000.044.4089781
TCAACGC1742400.043.33959212
CAACGCA1743700.043.2991913
AACGCAG1752150.043.094414
ATCAACG1758600.043.09001511
AGTGGTA1753900.043.0875785
GTGGTAT1754050.043.083896
ACGCAGA1751650.043.08120715
GTATCAA1758600.043.0512669
GGTATCA1761550.042.9564868
TGGTATC1764400.042.8205077
TATCAAC1766050.042.73259710
CGCAGAG1768500.042.68136616
AGCAGTG1790350.042.5936282
AGAGTAC1752200.042.56608619
GCAGTGG1795250.042.3281443
CAGTGGT1795500.042.1154564
CAGAGTA1771450.042.11016518
GAGTACT633100.041.8701620
GCAGAGT1795600.041.76890617