##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805420_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18589801
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.19046470696485	37.0	35.0	37.0	35.0	37.0
2	36.16581791273613	37.0	35.0	37.0	35.0	37.0
3	37.87073062266777	39.0	38.0	39.0	35.0	39.0
4	37.827624297860964	39.0	38.0	39.0	35.0	39.0
5	37.73778433669086	39.0	38.0	39.0	35.0	39.0
6	37.74933276585371	39.0	38.0	39.0	35.0	39.0
7	37.65779698233456	39.0	37.0	39.0	35.0	39.0
8	38.56143973784335	40.0	39.0	40.0	36.0	40.0
9	38.52684081986676	40.0	39.0	40.0	36.0	40.0
10	38.307947782765396	40.0	38.0	40.0	34.0	40.0
11	38.454526274918166	40.0	38.0	40.0	35.0	40.0
12	38.602186758212206	40.0	39.0	40.0	36.0	40.0
13	38.67867294545003	40.0	39.0	40.0	36.0	40.0
14	38.66078362000755	40.0	39.0	40.0	36.0	40.0
15	38.63059448565372	40.0	39.0	40.0	36.0	40.0
16	38.47050175523665	40.0	39.0	40.0	35.0	40.0
17	38.47858043235643	40.0	39.0	40.0	35.0	40.0
18	38.40840614700502	40.0	38.0	40.0	35.0	40.0
19	38.41824229318001	40.0	38.0	40.0	35.0	40.0
20	38.35544221264122	40.0	38.0	40.0	35.0	40.0
21	38.28619671614559	40.0	38.0	40.0	35.0	40.0
22	38.27571489334394	40.0	38.0	40.0	34.0	40.0
23	37.83954841689806	40.0	38.0	40.0	34.0	40.0
24	38.10042850915941	40.0	38.0	40.0	34.0	40.0
25	38.19723212744451	40.0	38.0	40.0	34.0	40.0
26	38.16500273456397	40.0	38.0	40.0	34.0	40.0
27	38.20905748264868	40.0	38.0	40.0	34.0	40.0
28	38.193456992896266	40.0	38.0	40.0	34.0	40.0
29	38.11567310483851	40.0	38.0	40.0	34.0	40.0
30	37.98207812983044	40.0	38.0	40.0	34.0	40.0
31	37.93637936199532	40.0	38.0	40.0	34.0	40.0
32	37.88417019633508	40.0	38.0	40.0	33.0	40.0
33	37.773862452857884	40.0	38.0	40.0	33.0	40.0
34	37.77028888044579	40.0	38.0	40.0	33.0	40.0
35	37.683396933619676	40.0	38.0	40.0	33.0	40.0
36	37.61906843435279	40.0	38.0	40.0	33.0	40.0
37	37.517137596039895	40.0	37.0	40.0	33.0	40.0
38	37.38277655581144	40.0	37.0	40.0	33.0	40.0
39	37.34323100069764	40.0	37.0	40.0	33.0	40.0
40	37.28916070699197	40.0	37.0	40.0	33.0	40.0
41	37.23231598875103	40.0	36.0	40.0	33.0	40.0
42	37.16150850673442	40.0	36.0	40.0	32.0	40.0
43	37.04539123361245	40.0	36.0	40.0	32.0	40.0
44	36.93130792524352	39.0	36.0	40.0	31.0	40.0
45	36.801482221353524	39.0	35.0	40.0	31.0	40.0
46	36.723880422388596	39.0	35.0	40.0	31.0	40.0
47	36.50882384378402	39.0	35.0	40.0	31.0	40.0
48	36.44164598642019	39.0	35.0	40.0	31.0	40.0
49	36.27563371980152	39.0	35.0	40.0	30.0	40.0
50	36.1912315790793	38.0	35.0	40.0	30.0	40.0
51	36.00826130414199	38.0	35.0	40.0	30.0	40.0
52	35.7662994886282	38.0	35.0	40.0	29.0	40.0
53	35.538330829899685	38.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	9.0
13	28.0
14	77.0
15	286.0
16	677.0
17	1420.0
18	2707.0
19	4985.0
20	8857.0
21	14457.0
22	22552.0
23	33364.0
24	47670.0
25	65230.0
26	87130.0
27	113182.0
28	145031.0
29	181875.0
30	225619.0
31	279863.0
32	347711.0
33	437906.0
34	571226.0
35	781182.0
36	1144763.0
37	1903207.0
38	3366938.0
39	8801846.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.048832582984616	36.95515083781693	29.80473540303094	21.19128117616751
2	27.95871779369774	42.349969211612326	13.464130143189806	16.22718285150013
3	34.625480929032	26.026109693159167	19.006152889963694	20.342256487845138
4	22.08188242574517	24.32711356081757	20.271669395492722	33.31933461794454
5	20.65698282622821	33.52012751508206	23.737279382388223	22.085610276301505
6	30.60698713235284	23.90906712772235	22.243460271575795	23.240485468349014
7	22.79995358745368	23.30655395396648	31.78856513848642	22.10492732009342
8	30.078853453030508	25.050101396997203	23.499450047905302	21.371595102066987
9	30.325015313504434	24.326430390513597	23.809840675540315	21.53871362044166
10	21.523382633305218	24.858759918946955	32.06026250630655	21.557594941441277
11	21.322116358319274	33.36265944966274	23.867011809324907	21.448212382693068
12	21.15943037797984	24.53299526982564	32.498185429741824	21.809388922452694
13	21.20817753777999	24.715928911772643	23.943978744043577	30.131914806403792
14	21.684551652812207	33.47849178159573	23.519891364087222	21.317065201504846
15	21.417007099753246	33.113920907491156	23.567820871240095	21.901251121515504
16	21.918029138665872	24.208327996625677	23.787565020195753	30.086077844512698
17	30.395855232662257	24.1507910708673	23.7211630183669	21.732190678103546
18	21.690840047185013	24.331815063539413	23.727058724297265	30.250286164978313
19	21.808824096610824	32.88899111937777	23.710931601688472	21.59125318232293
20	30.539127341922594	24.473516419029984	23.705670652418494	21.281685586628925
21	21.91972899548521	32.79259417569882	23.72366976924605	21.564007059569924
22	30.17802611227522	24.10163508474351	24.09389428106304	21.626444521918227
23	21.805854726470713	24.392111566982347	32.32444500078296	21.477588705763985
24	21.7701577332646	32.54915423785333	23.7854939921089	21.895194036773173
25	21.58590616435324	24.292992700674958	23.85018537853095	30.270915756440857
26	21.910772471421293	29.659752678363798	26.911175649486513	21.5182992007284
27	22.072796798631682	24.04638435882127	32.316338405128704	21.564480437418347
28	27.425253234286906	24.038008798480416	26.89026095545617	21.64647701177651
29	27.59180154752598	24.17288383022497	26.691216328781568	21.544098293467478
30	27.478018726504928	24.039208380982668	26.76867815852359	21.714094733988816
31	25.270125269226927	24.968411442381765	27.34696837260388	22.414494915787426
32	24.150495209712037	25.772384545697935	27.5407520500085	22.536368194581534
33	23.85977127996152	25.272680433749667	27.890820348211363	22.976727938077442
34	23.34687176048845	25.193050748633617	28.19571333765219	23.26436415322574
35	23.335828070456483	25.359868026559298	28.238134448023406	23.066169454960814
36	23.335553726476146	25.31218058762437	28.30469244937049	23.047573236528997
37	23.592748518394576	25.16334628864505	28.279495837529407	22.96440935543097
38	23.78072255856854	25.313423204476475	28.15386243241657	22.751991804538413
39	23.691264903803972	25.208553873169485	28.18844053252641	22.911740690500128
40	23.3514065051046	25.483080749492693	28.02589979311774	23.139612952284967
41	23.662162924713396	25.7630729882477	27.803756479157578	22.771007607881334
42	23.566863356955782	25.636551999669067	27.94751810414754	22.84906653922761
43	23.42848102569791	25.44741603204897	28.324106320449584	22.799996621803537
44	23.670086624380758	25.411670625199267	27.973720643916522	22.944522106503452
45	23.720140952557802	25.045814099892734	28.052086195005533	23.181958752543935
46	23.483382097527564	25.191878062600026	27.895817712088473	23.428922127783938
47	23.50530809877954	25.520423806580823	27.82329407399251	23.150974020647126
48	23.437303067418526	25.17664390275076	27.97412947024016	23.41192355959055
49	23.232013080720982	25.589117387539545	27.867689385163402	23.31118014657607
50	23.741841023473032	25.55483514858497	27.652356257068057	23.050967570873944
51	23.594873339418747	25.356285416933726	27.82910371122316	23.219737532424364
52	23.743062123150217	25.38356919474286	27.62846681360387	23.244901868503057
53	24.068708427809423	25.508298878508707	27.206063152585656	23.216929541096217
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5464.0
1	3230.5
2	997.0
3	817.5
4	638.0
5	708.0
6	778.0
7	916.5
8	1304.0
9	1553.0
10	2313.5
11	3074.0
12	4639.0
13	6204.0
14	8132.5
15	10061.0
16	14817.0
17	19573.0
18	26968.0
19	34363.0
20	49653.5
21	64944.0
22	301884.5
23	538825.0
24	373541.5
25	218393.5
26	228529.0
27	251803.5
28	275078.0
29	304175.0
30	333272.0
31	362272.0
32	391272.0
33	437194.0
34	483116.0
35	540329.0
36	597542.0
37	658963.5
38	720385.0
39	779044.0
40	837703.0
41	898225.5
42	1015567.5
43	1072387.0
44	1127949.0
45	1183511.0
46	1223401.0
47	1263291.0
48	1297979.0
49	1332667.0
50	1340338.0
51	1348009.0
52	1329321.0
53	1310633.0
54	1272310.0
55	1233987.0
56	1183308.5
57	1132630.0
58	1021967.5
59	798782.0
60	686259.0
61	596484.5
62	506710.0
63	429688.5
64	352667.0
65	289050.5
66	225434.0
67	182099.5
68	138765.0
69	108911.0
70	79057.0
71	60903.0
72	42749.0
73	32496.5
74	22244.0
75	17137.5
76	9278.0
77	6525.0
78	4980.0
79	3435.0
80	2780.5
81	2126.0
82	1609.0
83	1092.0
84	773.5
85	455.0
86	312.0
87	169.0
88	140.0
89	111.0
90	85.5
91	60.0
92	48.5
93	22.5
94	8.0
95	8.0
96	8.0
97	6.5
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.8589801E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.737313992608264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.08722043564812	35.854247176621975
2	15.329172779714856	15.248637595832426
3	5.053788685536845	7.540856241145111
4	2.280584551722833	4.537205997429212
5	1.2284085471045112	3.05488708092704
6	0.7878958222435254	2.351269314263429
7	0.5236350606980568	1.8230941002034415
8	0.3823883411838829	1.521517519406032
9	0.2902825929817722	1.2994088826350618
>10	1.9097452517046964	17.169648927355585
>50	0.09459143633669989	3.20103177955167
>100	0.030625387836972835	2.5533601395328422
>500	0.0010032309655591243	0.34239247299730713
>1k	5.241064302332135E-4	0.516843931591009
>5k	5.5737454733986765E-5	0.19731078963895093
>10k+	7.803243662758079E-5	2.788288050868835
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	386333	2.078198685397439	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	29794	0.16027067745372853	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	21864	0.11761287815829766	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	21015	0.11304585777975784	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5599951823045335E-4	0.0	0.0	0.0	0.0
2	0.00542770737567336	0.0	0.0	5.379293732084598E-6	5.379293732084598E-6
3	0.005438465963137529	0.0	0.0	5.379293732084598E-6	5.379293732084598E-6
4	0.005562189718975475	0.0	0.0	5.379293732084598E-6	1.0758587464169197E-5
5	0.005578327600171728	0.0	0.0	5.379293732084598E-6	2.689646866042299E-5
6	0.005589086187635898	0.0	0.0	1.0758587464169197E-5	2.689646866042299E-5
7	0.005589086187635898	0.0	0.0	2.1517174928338393E-5	2.689646866042299E-5
8	0.005589086187635898	0.0	0.0	2.1517174928338393E-5	3.765505612459219E-5
9	0.005589086187635898	0.0	0.0	2.689646866042299E-5	3.765505612459219E-5
10	0.005594465481367983	0.0	0.0	3.227576239250759E-5	4.3034349856676786E-5
11	0.005610603362564236	0.0	0.0	3.227576239250759E-5	5.379293732084598E-5
12	0.005745085705866351	0.0	0.0	3.227576239250759E-5	5.379293732084598E-5
13	0.0057504649995984355	0.0	0.0	3.227576239250759E-5	5.379293732084598E-5
14	0.005761223587062605	0.0	0.0	3.227576239250759E-5	6.455152478501518E-5
15	0.005761223587062605	0.0	0.0	6.993081851709978E-5	6.993081851709978E-5
16	0.005777361468258859	0.0	0.0	7.531011224918438E-5	7.531011224918438E-5
17	0.0057988786431871974	0.0	0.0	7.531011224918438E-5	7.531011224918438E-5
18	0.005820395818115535	0.0	0.0	9.144799344543817E-5	7.531011224918438E-5
19	0.005831154405579705	0.0	0.0	9.682728717752277E-5	7.531011224918438E-5
20	0.005841912993043874	5.379293732084598E-6	0.0	1.0220658090960737E-4	9.682728717752277E-5
21	0.005863430167972212	5.379293732084598E-6	0.0	1.0220658090960737E-4	9.682728717752277E-5
22	0.005884947342900551	5.379293732084598E-6	0.0	1.2372375583794576E-4	1.0220658090960737E-4
23	0.005922602399025143	5.379293732084598E-6	0.0	1.2372375583794576E-4	1.0220658090960737E-4
24	0.005971016042613905	5.379293732084598E-6	0.0	1.2372375583794576E-4	1.0220658090960737E-4
25	0.006019429686202666	5.379293732084598E-6	0.0	1.2910304957003036E-4	1.0220658090960737E-4
26	0.006057084742327258	5.379293732084598E-6	0.0	1.3448234330211496E-4	1.0758587464169197E-4
27	0.006110877679648104	5.379293732084598E-6	0.0	1.3448234330211496E-4	1.1834446210586116E-4
28	0.006153912029504781	5.379293732084598E-6	0.0	1.3448234330211496E-4	1.2372375583794576E-4
29	0.00622384284802188	5.379293732084598E-6	0.0	1.3448234330211496E-4	1.2910304957003036E-4
30	0.006283015079074811	5.379293732084598E-6	0.0	1.4524093076628415E-4	1.3448234330211496E-4
31	0.006422876716109011	5.379293732084598E-6	0.0	1.5599951823045335E-4	1.3986163703419956E-4
32	0.006498186828358195	5.379293732084598E-6	0.0	1.5599951823045335E-4	1.3986163703419956E-4
33	0.006573496940607379	5.379293732084598E-6	0.0	1.6137881196253795E-4	1.4524093076628415E-4
34	0.006729496458837833	5.379293732084598E-6	0.0	1.7213739942670715E-4	1.4524093076628415E-4
35	0.007009219732906232	5.379293732084598E-6	0.0	1.8289598689087634E-4	1.4524093076628415E-4
36	0.007063012670227077	5.379293732084598E-6	0.0	1.8827528062296094E-4	1.5599951823045335E-4
37	0.0071006677263516695	5.379293732084598E-6	0.0	1.8827528062296094E-4	1.5599951823045335E-4
38	0.007208253600993362	5.379293732084598E-6	0.0	2.0979245555129933E-4	1.5599951823045335E-4
39	0.0072997015944388	5.379293732084598E-6	0.0	2.0979245555129933E-4	1.5599951823045335E-4
40	0.007434183937740915	5.379293732084598E-6	0.0	2.1517174928338393E-4	1.6675810569462255E-4
41	0.007536390518650522	5.379293732084598E-6	0.0	2.1517174928338393E-4	1.6675810569462255E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	171600	0.0	44.408978	1
TCAACGC	174240	0.0	43.339592	12
CAACGCA	174370	0.0	43.29919	13
AACGCAG	175215	0.0	43.0944	14
ATCAACG	175860	0.0	43.090015	11
AGTGGTA	175390	0.0	43.087578	5
GTGGTAT	175405	0.0	43.08389	6
ACGCAGA	175165	0.0	43.081207	15
GTATCAA	175860	0.0	43.051266	9
GGTATCA	176155	0.0	42.956486	8
TGGTATC	176440	0.0	42.820507	7
TATCAAC	176605	0.0	42.732597	10
CGCAGAG	176850	0.0	42.681366	16
AGCAGTG	179035	0.0	42.593628	2
AGAGTAC	175220	0.0	42.566086	19
GCAGTGG	179525	0.0	42.328144	3
CAGTGGT	179550	0.0	42.115456	4
CAGAGTA	177145	0.0	42.110165	18
GAGTACT	63310	0.0	41.87016	20
GCAGAGT	179560	0.0	41.768906	17
>>END_MODULE