FastQCFastQC Report
Thu 26 May 2016
SRR805419_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805419_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17658967
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT518030.2933523801250662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT294500.035.1422731
TCAACGC390050.027.08780912
CAACGCA390050.027.07575813
AGCAGTG397800.026.8731772
ACGCAGA395600.026.67214415
AACGCAG397000.026.64319814
AGTGGTA397900.026.5770285
GGTATCA403100.026.2633328
GTGGTAT405800.026.0596356
GTATCAA407550.026.0111649
CGCAGAG406900.025.96608716
ATCAACG408200.025.94671611
GCAGTGG411550.025.9410743
TGGTATC408750.025.8485647
TATCAAC419400.025.23700310
AGAGTAC417950.025.0265619
CAGTGGT436500.024.3290944
CAGAGTA438900.023.94440818
GCAGAGT444250.023.75126617
GTACATG300700.022.53092822
Produced by FastQC (version 0.11.5)