##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805419_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17658967
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.42230097604237	36.0	35.0	37.0	35.0	37.0
2	35.83460402865015	37.0	35.0	37.0	35.0	37.0
3	37.734355469377114	39.0	38.0	39.0	35.0	39.0
4	37.80334449914313	39.0	38.0	39.0	35.0	39.0
5	37.78574573473069	39.0	38.0	39.0	35.0	39.0
6	37.770193239502625	39.0	38.0	39.0	35.0	39.0
7	37.788984485898865	39.0	38.0	39.0	35.0	39.0
8	38.649061465486625	40.0	39.0	40.0	36.0	40.0
9	38.613710077152305	40.0	39.0	40.0	36.0	40.0
10	38.6110742491336	40.0	39.0	40.0	36.0	40.0
11	38.623903595266924	40.0	39.0	40.0	36.0	40.0
12	38.67955481201137	40.0	39.0	40.0	36.0	40.0
13	38.73407844298027	40.0	39.0	40.0	36.0	40.0
14	38.698009685391	40.0	39.0	40.0	36.0	40.0
15	38.64462156818119	40.0	39.0	40.0	36.0	40.0
16	38.48421614922322	40.0	39.0	40.0	35.0	40.0
17	38.54093016879187	40.0	39.0	40.0	35.0	40.0
18	38.463176243548105	40.0	39.0	40.0	35.0	40.0
19	38.464024707674014	40.0	39.0	40.0	35.0	40.0
20	38.38370653277737	40.0	38.0	40.0	35.0	40.0
21	38.369160381804896	40.0	38.0	40.0	35.0	40.0
22	38.312835909371145	40.0	38.0	40.0	35.0	40.0
23	38.239652523276135	40.0	38.0	40.0	34.0	40.0
24	38.28097073854887	40.0	38.0	40.0	35.0	40.0
25	38.273704231963286	40.0	38.0	40.0	35.0	40.0
26	38.19648895657373	40.0	38.0	40.0	34.0	40.0
27	38.24127889247429	40.0	38.0	40.0	35.0	40.0
28	38.223047871373225	40.0	38.0	40.0	35.0	40.0
29	38.153328730950115	40.0	38.0	40.0	34.0	40.0
30	38.03052539822969	40.0	38.0	40.0	34.0	40.0
31	38.02058359359299	40.0	38.0	40.0	34.0	40.0
32	37.969212581913766	40.0	38.0	40.0	34.0	40.0
33	37.90756684691692	40.0	38.0	40.0	34.0	40.0
34	37.88346639981829	40.0	38.0	40.0	34.0	40.0
35	37.80587488498053	40.0	38.0	40.0	33.0	40.0
36	37.74720423907016	40.0	38.0	40.0	33.0	40.0
37	37.705545856674405	40.0	38.0	40.0	33.0	40.0
38	37.61343950639921	40.0	38.0	40.0	33.0	40.0
39	37.59583400320076	40.0	38.0	40.0	33.0	40.0
40	37.50399080535119	40.0	37.0	40.0	33.0	40.0
41	37.47433238875185	40.0	37.0	40.0	33.0	40.0
42	37.41798316968371	40.0	37.0	40.0	33.0	40.0
43	37.32160720386419	40.0	37.0	40.0	33.0	40.0
44	37.2391505686601	40.0	37.0	40.0	32.0	40.0
45	37.113111033051936	40.0	37.0	40.0	32.0	40.0
46	37.023695666909624	39.0	36.0	40.0	31.0	40.0
47	36.837723293780435	39.0	36.0	40.0	31.0	40.0
48	36.8110640333605	39.0	36.0	40.0	31.0	40.0
49	36.67631538130175	39.0	36.0	40.0	31.0	40.0
50	36.60798494045547	39.0	36.0	40.0	31.0	40.0
51	36.485285860718804	39.0	35.0	40.0	31.0	40.0
52	36.32643098545912	39.0	35.0	40.0	30.0	40.0
53	36.1859312608716	38.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	12.0
11	29.0
12	65.0
13	135.0
14	211.0
15	487.0
16	1060.0
17	2005.0
18	3571.0
19	5774.0
20	9717.0
21	15383.0
22	23128.0
23	33243.0
24	45655.0
25	61009.0
26	79209.0
27	101255.0
28	126836.0
29	158306.0
30	195785.0
31	242523.0
32	301845.0
33	377566.0
34	487388.0
35	659398.0
36	957517.0
37	1550419.0
38	3195132.0
39	9024298.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.270557275519005	28.27340353487268	27.333631689781175	30.12240749982714
2	23.961599792332134	25.688846918395626	30.25182050569549	20.097732783576753
3	17.3451652070022	25.740412788584973	32.2049641975094	24.70945780690343
4	17.80936563276889	28.17192534535004	31.474972460167123	22.543736561713946
5	23.16628713333005	28.532439071889087	29.107891758334446	19.193382036446412
6	22.607268024228144	26.90267782934302	29.66335460052675	20.82669954590209
7	23.075630641361975	27.519905326285503	29.341263280009528	20.06320075234299
8	22.06535637107199	26.04085505114767	29.285433287235886	22.60835529054446
9	21.69838700077983	25.44271700604005	30.87341971928483	21.985476273895298
10	21.735240798626556	26.767047019228247	29.172901223497387	22.32481095864781
11	21.594422822127704	28.033361181319382	28.222556846048807	22.149659150504103
12	21.70912941849883	26.94627041321273	28.851076056713847	22.49352411157459
13	21.923196300213938	27.342199574867543	27.19793858836703	23.53666553655149
14	22.32874097335365	28.348175745500853	27.083690682473105	22.239392598672392
15	22.11000790703103	28.01436233501088	27.12490487127588	22.750724886682217
16	22.727575174697364	26.901664180016873	26.885038065929905	23.48572257935586
17	23.94476415296546	26.552062756558747	26.88132890219456	22.62184418828123
18	22.50142378090406	26.89363426524326	26.841354876533828	23.76358707731885
19	22.911238239473462	27.8630850830629	26.730340455361855	22.49533622210178
20	23.83972969653321	26.806024384099025	26.88800539691818	22.466240522449585
21	22.803672491148546	27.840320444565076	26.98251828660193	22.373488777684447
22	23.654673571789335	26.537645152176797	27.167682005408356	22.639999270625513
23	22.57589019788077	26.910634127126464	28.19877289537944	22.314702779613327
24	22.36708409953991	27.774501192510296	27.023692835486923	22.834721872462868
25	22.49524561657542	26.71303479982719	27.00387287659578	23.787846707001602
26	22.67296269368418	27.166940172661292	27.803053259004333	22.3570438746502
27	22.81981726337673	26.336240392770428	28.37618984168213	22.46775250217071
28	23.584969607791894	26.39768226533296	27.3539329905311	22.663415136344046
29	23.698832440198796	26.47101045038478	27.40208982779117	22.42806728162525
30	23.42552653278077	26.417456921460918	27.462529376718354	22.694487169039956
31	23.120168920413068	26.64474088433372	27.35077312279931	22.8843170724539
32	23.117139298125423	26.828681428534296	27.421292536533986	22.63288673680629
33	22.916113949360685	26.74653053035322	27.44932362125146	22.88803189903464
34	22.970024237544585	26.700304723373687	27.34356998345373	22.986101055628
35	23.077465403270757	26.760999100343753	27.381103322748153	22.780432173637337
36	22.93126206079891	26.699829044360296	27.432957998052775	22.935950896788018
37	23.002998986294045	26.754396222610303	27.233371012018992	23.009233779076656
38	23.168405037508705	26.813493677178286	27.340840491972152	22.677260793340857
39	22.9876583381123	26.73786637689509	27.385667576138513	22.88880770885409
40	23.045889377334472	26.76729052158034	27.227090916473202	22.959729184611987
41	23.052905642781937	26.840947151665212	27.390877393904184	22.715269811648668
42	22.920672539905645	26.747232723182506	27.467518343513525	22.864576393398323
43	22.963948004433103	26.767794514820714	27.294218285814793	22.974039194931393
44	23.124200866336068	26.696850387681227	27.323750024562592	22.855198721420113
45	22.97117945800567	26.59717864584038	27.436933315521795	22.99470858063215
46	22.984787275495787	26.666474884969205	27.277473251974477	23.07126458756053
47	23.009284744685235	26.930442760326805	27.22322885591213	22.83704363907583
48	22.956801493541494	26.64743073589752	27.3615325290545	23.034235241506483
49	23.066286946456156	26.761916481298144	27.139679234917875	23.03211733732783
50	23.177607161279592	26.776951335828418	27.235721092858945	22.809720410033044
51	23.067917845930623	26.682800868250105	27.26190608997684	22.98737519584243
52	23.151405175625506	26.76534816560901	26.97802198735634	23.10522467140915
53	23.181622118666397	26.802654991087532	27.059634915224656	22.956087975021415
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9734.0
1	6149.0
2	2564.0
3	2193.0
4	1822.0
5	1943.5
6	2065.0
7	2377.0
8	3381.5
9	4074.0
10	5429.0
11	6784.0
12	9458.0
13	12132.0
14	16487.0
15	20842.0
16	29459.0
17	38076.0
18	51932.5
19	65789.0
20	88659.0
21	111529.0
22	173817.5
23	236106.0
24	233264.0
25	270489.5
26	310557.0
27	354992.0
28	399427.0
29	435599.0
30	471771.0
31	504206.5
32	536642.0
33	581743.0
34	626844.0
35	688774.5
36	750705.0
37	811215.5
38	871726.0
39	919858.0
40	967990.0
41	1010962.5
42	1087676.5
43	1121418.0
44	1142677.0
45	1163936.0
46	1175730.5
47	1187525.0
48	1184983.0
49	1182441.0
50	1168854.5
51	1155268.0
52	1120001.5
53	1084735.0
54	1031444.0
55	978153.0
56	908064.5
57	837976.0
58	759859.0
59	599181.0
60	516620.0
61	448803.0
62	380986.0
63	323643.5
64	266301.0
65	215390.0
66	164479.0
67	131715.5
68	98952.0
69	76095.0
70	53238.0
71	40090.0
72	26942.0
73	19623.5
74	12305.0
75	9058.0
76	4392.5
77	2974.0
78	2120.0
79	1266.0
80	1103.5
81	941.0
82	710.5
83	480.0
84	309.0
85	138.0
86	89.5
87	41.0
88	34.0
89	27.0
90	23.0
91	19.0
92	17.0
93	10.5
94	6.0
95	5.5
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.7658967E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.498155587909224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.23629964354875	35.46803587318937
2	16.93324775526252	17.10195559507723
3	5.685860575731104	8.61376416013285
4	2.479997782469244	5.0094125550720685
5	1.277169907989779	3.2247362362931775
6	0.7441810601402891	2.2547862576323725
7	0.47228841677728384	1.6694785766950615
8	0.34421731573011066	1.390587165263328
9	0.25357228040609103	1.1524439221854956
>10	1.4021176998717362	12.712457424338
>50	0.09408742548913397	3.282664964019718
>100	0.0751563590781856	6.938323585921448
>500	0.0014889959738826747	0.48593627672439593
>1k	2.798226383736637E-4	0.2561488518014873
>5k	1.165296450484088E-5	0.05140727282971323
>10k+	2.330592900968176E-5	0.3878612828241825
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51803	0.2933523801250662	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.361699016709188E-5	0.0	0.0	0.0	0.0
2	9.626837175696631E-5	5.662845397468606E-6	0.0	0.0	0.0
3	9.626837175696631E-5	1.1325690794937212E-5	0.0	5.662845397468606E-6	5.662845397468606E-6
4	9.626837175696631E-5	1.1325690794937212E-5	0.0	1.1325690794937212E-5	2.2651381589874425E-5
5	4.4736478640001987E-4	1.1325690794937212E-5	0.0	1.1325690794937212E-5	2.8314226987343032E-5
6	4.530276317974885E-4	1.6988536192405818E-5	0.0	1.1325690794937212E-5	3.3977072384811636E-5
7	4.8134185878483154E-4	2.2651381589874425E-5	0.0	2.2651381589874425E-5	3.3977072384811636E-5
8	4.870047041823001E-4	2.2651381589874425E-5	0.0	3.3977072384811636E-5	5.096560857721745E-5
9	4.870047041823001E-4	2.8314226987343032E-5	0.0	3.3977072384811636E-5	6.229129937215467E-5
10	5.153189311696432E-4	2.8314226987343032E-5	0.0	3.3977072384811636E-5	7.361699016709188E-5
11	5.266446219645804E-4	2.8314226987343032E-5	0.0	3.3977072384811636E-5	7.361699016709188E-5
12	5.719473851443292E-4	3.3977072384811636E-5	0.0	3.3977072384811636E-5	7.927983556456049E-5
13	6.455643753114211E-4	3.3977072384811636E-5	0.0	4.530276317974885E-5	1.0759406255190352E-4
14	7.361699016709188E-4	3.3977072384811636E-5	0.0	5.096560857721745E-5	1.1891975334684072E-4
15	8.041240464405421E-4	3.3977072384811636E-5	0.0	5.096560857721745E-5	1.1891975334684072E-4
16	8.211125826329479E-4	3.3977072384811636E-5	0.0	5.096560857721745E-5	1.1891975334684072E-4
17	8.834038820051026E-4	3.9639917782280246E-5	0.0	5.096560857721745E-5	1.2458259874430935E-4
18	8.947295728000397E-4	3.9639917782280246E-5	0.0	5.6628453974686064E-5	1.2458259874430935E-4
19	9.173809543899142E-4	4.530276317974885E-5	0.0	6.229129937215467E-5	1.4723398033418376E-4
20	9.570208721721944E-4	4.530276317974885E-5	0.0	6.229129937215467E-5	1.5289682573165237E-4
21	0.0010136493261468805	4.530276317974885E-5	0.0	7.361699016709188E-5	1.755482073215268E-4
22	0.0010249750169418178	4.530276317974885E-5	0.0	7.361699016709188E-5	1.755482073215268E-4
23	0.0010306378623392864	4.530276317974885E-5	0.0	7.361699016709188E-5	2.0952527970633842E-4
24	0.0010419635531342235	4.530276317974885E-5	0.0	7.927983556456049E-5	2.1518812510380703E-4
25	0.0010532892439291607	4.530276317974885E-5	0.0	7.927983556456049E-5	2.2651381589874426E-4
26	0.0010985920071089096	4.530276317974885E-5	0.0	7.927983556456049E-5	2.2651381589874426E-4
27	0.0011382319248911899	4.530276317974885E-5	0.0	8.494268096202909E-5	2.3217666129621287E-4
28	0.0011665461518785328	4.530276317974885E-5	0.0	9.06055263594977E-5	2.3783950669368145E-4
29	0.0012571516782380306	4.530276317974885E-5	0.0	9.06055263594977E-5	2.4350235209115006E-4
30	0.001336431513802591	5.096560857721745E-5	0.0	9.06055263594977E-5	2.4350235209115006E-4
31	0.0013987228131747457	5.096560857721745E-5	0.0	9.06055263594977E-5	2.4350235209115006E-4
32	0.0014440255763544946	5.096560857721745E-5	0.0	9.626837175696631E-5	2.548280428860873E-4
33	0.001523305411919055	5.096560857721745E-5	0.0	9.626837175696631E-5	2.661537336810245E-4
34	0.0015742710204962726	5.096560857721745E-5	0.0	1.019312171544349E-4	2.661537336810245E-4
35	0.001681865083048176	5.096560857721745E-5	0.0	1.019312171544349E-4	2.944679606683675E-4
36	0.0017215050008304563	5.096560857721745E-5	0.0	1.019312171544349E-4	3.1145649686077333E-4
37	0.0017894591456000795	5.096560857721745E-5	0.0	1.019312171544349E-4	3.1145649686077333E-4
38	0.0019083788989469202	5.096560857721745E-5	0.0	1.1325690794937213E-4	3.2278218765571055E-4
39	0.0020669385700760414	5.6628453974686064E-5	0.0	1.2458259874430935E-4	3.2844503305317913E-4
40	0.002146218405640602	5.6628453974686064E-5	0.0	1.2458259874430935E-4	3.3410787845064777E-4
41	0.0022198353958076938	5.6628453974686064E-5	0.0	1.4157113493671515E-4	3.567592600405222E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	29450	0.0	35.142273	1
TCAACGC	39005	0.0	27.087809	12
CAACGCA	39005	0.0	27.075758	13
AGCAGTG	39780	0.0	26.873177	2
ACGCAGA	39560	0.0	26.672144	15
AACGCAG	39700	0.0	26.643198	14
AGTGGTA	39790	0.0	26.577028	5
GGTATCA	40310	0.0	26.263332	8
GTGGTAT	40580	0.0	26.059635	6
GTATCAA	40755	0.0	26.011164	9
CGCAGAG	40690	0.0	25.966087	16
ATCAACG	40820	0.0	25.946716	11
GCAGTGG	41155	0.0	25.941074	3
TGGTATC	40875	0.0	25.848564	7
TATCAAC	41940	0.0	25.237003	10
AGAGTAC	41795	0.0	25.02656	19
CAGTGGT	43650	0.0	24.329094	4
CAGAGTA	43890	0.0	23.944408	18
GCAGAGT	44425	0.0	23.751266	17
GTACATG	30070	0.0	22.530928	22
>>END_MODULE