FastQCFastQC Report
Thu 26 May 2016
SRR805418_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805418_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24801355
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7433272.997122536248524No Hit
AAGCAGTGGTATCAAAGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1353070.5455629339606647No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG339250.13678688120064408No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA322390.12998886552771008No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG283300.11422762990167272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3039200.045.2323951
GTGGTAT3100550.044.019066
AGTGGTA3110550.043.9636735
GTATCAA3108050.043.9135939
GGTATCA3107750.043.9042248
AGCAGTG3133750.043.843672
TGGTATC3112950.043.8339047
TCAACGC2645650.043.7507812
CAACGCA2646750.043.7317113
GCAGTGG3140700.043.703253
AACGCAG2654400.043.58176814
ACGCAGA2652350.043.53925315
ATCAACG2661550.043.50971611
AGAGTAC3093250.043.42400419
GAGTACT1359650.043.36347220
CGCAGAG2668100.043.2743116
CAGTGGT3163050.043.2636834
TATCAAC2680200.043.08157710
CAGAGTA3128650.043.0671218
GCAGAGT3147600.043.02733217