FastQCFastQC Report
Thu 26 May 2016
SRR805417_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805417_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22259200
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3830531.7208749640598047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1742150.044.169991
TCAACGC1496600.042.78080412
CAACGCA1497750.042.73383313
GGTATCA1788300.042.6937078
GTATCAA1789500.042.6624459
TGGTATC1790600.042.636247
GTGGTAT1791950.042.6028066
AGTGGTA1796450.042.5941965
AACGCAG1506900.042.44472514
ACGCAGA1506000.042.4217115
ATCAACG1512300.042.33511711
AGAGTAC1783300.042.11498319
CGCAGAG1523350.041.9370116
AGCAGTG1842750.041.7382472
GCAGTGG1845850.041.628683
CAGAGTA1810200.041.62415718
GCAGAGT1822050.041.5997917
CAGTGGT1843650.041.5521664
GAGTACT742500.041.5182520
TATCAAC1538200.041.5153410
Produced by FastQC (version 0.11.5)