##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805417_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22259200
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.5556642646636	35.0	35.0	35.0	32.0	37.0
2	35.4869663779471	35.0	35.0	37.0	35.0	37.0
3	37.85081683079356	39.0	37.0	39.0	35.0	39.0
4	37.88854222074468	39.0	38.0	39.0	35.0	39.0
5	37.85378620974698	39.0	38.0	39.0	35.0	39.0
6	37.84277557144911	39.0	38.0	39.0	35.0	39.0
7	37.7811053856383	39.0	38.0	39.0	35.0	39.0
8	38.76504339778609	40.0	39.0	40.0	36.0	40.0
9	38.78201903033352	40.0	39.0	40.0	36.0	40.0
10	38.56745309804485	40.0	38.0	40.0	36.0	40.0
11	38.64086481095457	40.0	39.0	40.0	36.0	40.0
12	38.66571462586256	40.0	39.0	40.0	36.0	40.0
13	38.7970384380391	40.0	39.0	40.0	36.0	40.0
14	38.73582181749569	40.0	39.0	40.0	36.0	40.0
15	38.72803865368028	40.0	39.0	40.0	36.0	40.0
16	32.762680015454286	40.0	36.0	40.0	3.0	40.0
17	35.124967519048305	40.0	36.0	40.0	18.0	40.0
18	37.182835906052325	40.0	36.0	40.0	32.0	40.0
19	37.970937005822314	40.0	37.0	40.0	34.0	40.0
20	38.20753261572743	40.0	38.0	40.0	35.0	40.0
21	38.22307194328637	40.0	38.0	40.0	35.0	40.0
22	38.3377816363571	40.0	38.0	40.0	35.0	40.0
23	37.991377138441635	40.0	38.0	40.0	34.0	40.0
24	38.148968875790686	40.0	38.0	40.0	34.0	40.0
25	38.20431866374353	40.0	38.0	40.0	34.0	40.0
26	38.192570802185166	40.0	38.0	40.0	34.0	40.0
27	38.17529435020126	40.0	38.0	40.0	34.0	40.0
28	38.0561025553479	40.0	38.0	40.0	34.0	40.0
29	37.8621466180276	40.0	38.0	40.0	34.0	40.0
30	37.78290554018114	40.0	38.0	40.0	33.0	40.0
31	37.84312019299885	40.0	38.0	40.0	33.0	40.0
32	37.89440662737205	40.0	38.0	40.0	34.0	40.0
33	37.88954149295572	40.0	38.0	40.0	34.0	40.0
34	37.91117223440195	40.0	38.0	40.0	34.0	40.0
35	37.84733085645486	40.0	38.0	40.0	33.0	40.0
36	37.90849199432145	40.0	38.0	40.0	34.0	40.0
37	37.78574737636573	40.0	38.0	40.0	33.0	40.0
38	37.76885481059517	40.0	38.0	40.0	33.0	40.0
39	37.79492910796435	40.0	38.0	40.0	33.0	40.0
40	37.75715753486199	40.0	38.0	40.0	33.0	40.0
41	37.67974262327487	40.0	37.0	40.0	33.0	40.0
42	37.66966130858252	40.0	37.0	40.0	33.0	40.0
43	37.637514915181136	40.0	37.0	40.0	33.0	40.0
44	37.55287296937895	40.0	37.0	40.0	33.0	40.0
45	37.46280562643761	40.0	37.0	40.0	33.0	40.0
46	37.35231495291834	40.0	37.0	40.0	33.0	40.0
47	37.13406555491662	40.0	36.0	40.0	32.0	40.0
48	37.11677283999425	40.0	36.0	40.0	32.0	40.0
49	37.050272246981024	40.0	36.0	40.0	32.0	40.0
50	36.98120529938183	40.0	36.0	40.0	32.0	40.0
51	36.864410535868316	39.0	36.0	40.0	31.0	40.0
52	36.69197554269695	39.0	35.0	40.0	31.0	40.0
53	36.13885624820299	39.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	6.0
12	25.0
13	57.0
14	155.0
15	360.0
16	954.0
17	1866.0
18	3505.0
19	6335.0
20	11183.0
21	17846.0
22	27086.0
23	38644.0
24	53425.0
25	71768.0
26	94678.0
27	121479.0
28	155228.0
29	196907.0
30	246302.0
31	312505.0
32	393008.0
33	507069.0
34	675045.0
35	956338.0
36	1474838.0
37	2752429.0
38	4527780.0
39	9612374.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.195074396204715	44.53164084962622	21.517480412593446	21.75580434157562
2	26.2966234186314	41.1481859186314	18.137435307648072	14.417755355089131
3	32.34459010207015	25.249636105520413	22.285127048591143	20.120646743818284
4	20.82299004456584	26.00321664749856	22.350106922081654	30.82368638585394
5	19.647134667912596	33.83900140166762	25.760521492236915	20.753342438182866
6	28.360700294709602	24.37870184013801	24.58925298303623	22.67134488211616
7	22.750107820586543	24.832127839275444	31.405351495112132	21.012412845025878
8	27.645319688039105	25.47414552185164	25.657696592869467	21.222838197239792
9	28.29599895773433	24.641397714203563	25.935770378090854	21.12683294997125
10	21.26044961184589	25.774812212478437	31.867331260782063	21.097406914893618
11	20.90175298303623	32.810689512650946	25.14337442495687	21.14418307935595
12	21.04923806785509	25.14830721679126	32.17049579499712	21.631958920356524
13	21.019160616733757	25.916043703277747	24.691309660724553	28.373486019263943
14	21.408774798734907	33.082231167337554	24.548676502300175	20.960317531627375
15	21.241280010063253	32.574162593444505	24.49547153536515	21.689085861127086
16	18.03481257188039	37.599212909718226	20.53076929988499	23.83520521851639
17	28.735538563829788	25.089419206440482	24.727416079643476	21.447626150086258
18	21.36919116589994	25.151110552041406	24.600915576480737	28.878782705577915
19	21.72567298016101	32.58112151380104	24.47743404974123	21.21577145629672
20	28.930828601207594	25.451283064979872	24.58121585681426	21.036672476998277
21	21.875956907705575	32.22162970816561	24.57840802903968	21.32400535508913
22	28.63511716503738	25.15410706584244	24.934278859977	21.276496909143187
23	21.46990907130535	25.342411227717076	32.04712208884416	21.14055761213341
24	21.603013585393903	32.00045374496837	24.55918451696377	21.83734815267395
25	21.36515238642898	25.29241841575618	24.674408783783782	28.668020414031055
26	21.553919278320873	29.33823767251294	27.97563254744106	21.13221050172513
27	21.88562931282346	24.72677364864865	31.922476998274867	21.46512004025302
28	26.00234959028177	25.042112924094305	27.56033909574468	21.39519838987924
29	26.25769569436458	24.962464958309376	27.622677364864867	21.157161982461183
30	26.078089059804487	24.75983862852214	27.63482065842438	21.527251653248992
31	24.172175999137437	25.801363031914892	27.94711400230017	22.0793469666475
32	23.25432180851064	26.371608144048302	28.336112708453133	22.037957338987923
33	23.193803011788383	25.776020701552614	28.45499389016676	22.575182396492238
34	22.754222972972972	26.040374317136283	28.58600488786659	22.61939782202415
35	22.683820622484184	26.020777925531913	28.8331161946521	22.4622852573318
36	22.813605161012077	25.786551178838412	28.748957734330077	22.650885925819438
37	22.9446386213341	26.00188236774008	28.557073030477287	22.496405980448532
38	22.965614217941347	26.019555958884418	28.76575977573318	22.24907004744106
39	22.993112960034505	25.774852645198386	28.713920536227715	22.51811385853939
40	22.756226638872914	26.197760925819434	28.474455506037955	22.571556929269697
41	22.889434481023578	26.302171686313976	28.517615188326623	22.290778644335827
42	23.002915648361128	25.92017233323749	28.55772444652099	22.51918757188039
43	22.850776308223118	26.189148756469233	28.621405980448532	22.338668954859113
44	22.925801466359978	26.073057432432435	28.65491572024152	22.34622538096607
45	23.037207087406557	25.661672476998277	28.5895584746981	22.71156196089707
46	22.847901092581942	25.985385817998853	28.434746082518693	22.731967006900515
47	22.81931965209891	26.220313398504885	28.479621909143187	22.48074504025302
48	22.95306210465785	25.67768383410006	28.55813775876941	22.811116302472687
49	22.753122304485338	26.22205200546291	28.325734976998273	22.69909071305348
50	23.05521761788384	26.120489505462906	28.394632331799883	22.429660544853363
51	23.0634254600345	25.811134272570442	28.379061242093158	22.746379025301895
52	23.09294134560092	26.14257924813111	28.069144443645772	22.695334962622198
53	23.25821682719954	26.22460825186889	27.90229208596895	22.61488283496262
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8559.0
1	5132.5
2	1706.0
3	1462.0
4	1218.0
5	1278.0
6	1338.0
7	1598.5
8	2332.0
9	2805.0
10	4215.0
11	5625.0
12	8418.0
13	11211.0
14	14855.5
15	18500.0
16	26257.0
17	34014.0
18	47137.5
19	60261.0
20	124166.0
21	188071.0
22	398211.5
23	608352.0
24	453799.5
25	321170.0
26	343093.0
27	381515.5
28	419938.0
29	463777.0
30	507616.0
31	549052.0
32	590488.0
33	650443.5
34	710399.0
35	787289.0
36	864179.0
37	933905.0
38	1003631.0
39	1069830.5
40	1136030.0
41	1196845.5
42	1313285.0
43	1368909.0
44	1414145.5
45	1459382.0
46	1483795.5
47	1508209.0
48	1518993.0
49	1529777.0
50	1523084.0
51	1516391.0
52	1477969.0
53	1439547.0
54	1377002.0
55	1314457.0
56	1232692.0
57	1150927.0
58	1032460.0
59	799228.5
60	684464.0
61	589746.5
62	495029.0
63	415351.0
64	335673.0
65	271531.0
66	207389.0
67	165265.5
68	123142.0
69	95385.0
70	67628.0
71	51003.0
72	34378.0
73	25689.0
74	17000.0
75	12792.5
76	6464.5
77	4344.0
78	3187.5
79	2031.0
80	1589.5
81	1148.0
82	866.5
83	585.0
84	399.5
85	214.0
86	147.0
87	80.0
88	63.5
89	47.0
90	41.0
91	35.0
92	22.5
93	10.0
94	10.0
95	9.5
96	9.0
97	6.0
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.22592E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.901303845747066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.693175617057506	29.23599806899085
2	19.923476353662988	18.290270835460834
3	7.13740814904196	9.828490203608586
4	3.172571232983908	5.825006245498832
5	1.5972568551642627	3.665808611429862
6	0.9815379582909379	2.703232323578793
7	0.6326427020260128	2.032738742404331
8	0.4495141180975238	1.6506627294197975
9	0.3225173278967351	1.3323569276975826
>10	1.9340503177825195	15.782634571928094
>50	0.10625625507521001	3.3496435548495587
>100	0.048365451057956685	3.5087982386301952
>500	7.343147861787396E-4	0.22740516274757927
>1k	3.849933457512619E-4	0.3076745502559921
>5k	2.955101742390405E-5	0.09444371546169321
>10k+	7.880271313040837E-5	2.1648355180373735
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	383053	1.7208749640598047	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.941776883266245E-5	0.0	0.0	0.0	8.9850488786659E-6
2	4.941776883266245E-5	0.0	0.0	0.0	1.347757331799885E-5
3	4.941776883266245E-5	4.49252443933295E-6	0.0	0.0	1.347757331799885E-5
4	5.840281771132835E-5	4.49252443933295E-6	0.0	1.347757331799885E-5	1.79700977573318E-5
5	5.840281771132835E-5	4.49252443933295E-6	0.0	8.535796434732605E-5	2.246262219666475E-5
6	5.840281771132835E-5	4.49252443933295E-6	0.0	8.9850488786659E-5	2.246262219666475E-5
7	5.840281771132835E-5	4.49252443933295E-6	0.0	1.617308798159862E-4	2.246262219666475E-5
8	5.840281771132835E-5	8.9850488786659E-6	0.0	1.617308798159862E-4	2.246262219666475E-5
9	5.840281771132835E-5	8.9850488786659E-6	0.0	1.617308798159862E-4	2.246262219666475E-5
10	5.840281771132835E-5	8.9850488786659E-6	0.0	1.617308798159862E-4	2.69551466359977E-5
11	5.840281771132835E-5	8.9850488786659E-6	0.0	1.617308798159862E-4	3.144767107533065E-5
12	5.840281771132835E-5	8.9850488786659E-6	0.0	1.617308798159862E-4	3.144767107533065E-5
13	6.28953421506613E-5	8.9850488786659E-6	0.0	1.617308798159862E-4	3.144767107533065E-5
14	6.738786658999425E-5	8.9850488786659E-6	0.0	1.617308798159862E-4	3.144767107533065E-5
15	7.18803910293272E-5	8.9850488786659E-6	0.0	1.707159286946521E-4	3.144767107533065E-5
16	8.08654399079931E-5	8.9850488786659E-6	0.0	1.707159286946521E-4	3.144767107533065E-5
17	8.9850488786659E-5	8.9850488786659E-6	0.0	1.7520845313398505E-4	4.043271995399655E-5
18	9.88355376653249E-5	8.9850488786659E-6	0.0	1.8419350201265096E-4	4.043271995399655E-5
19	1.078205865439908E-4	8.9850488786659E-6	0.0	1.886860264519839E-4	4.49252443933295E-5
20	1.2129815986198964E-4	8.9850488786659E-6	0.0	1.886860264519839E-4	5.39102932719954E-5
21	1.257906843013226E-4	1.347757331799885E-5	0.0	1.886860264519839E-4	5.840281771132835E-5
22	1.3028320874065554E-4	1.347757331799885E-5	0.0	1.9317855089131684E-4	6.28953421506613E-5
23	1.4825330649798735E-4	1.347757331799885E-5	0.0	2.066561242093157E-4	6.28953421506613E-5
24	1.5723835537665324E-4	1.79700977573318E-5	0.0	2.156411730879816E-4	7.18803910293272E-5
25	1.79700977573318E-4	1.79700977573318E-5	0.0	2.2013369752731454E-4	7.637291546866015E-5
26	2.066561242093157E-4	1.79700977573318E-5	0.0	2.2013369752731454E-4	7.637291546866015E-5
27	2.2911874640598045E-4	2.246262219666475E-5	0.0	2.2013369752731454E-4	8.08654399079931E-5
28	2.515813686026452E-4	2.69551466359977E-5	0.0	2.2013369752731454E-4	8.9850488786659E-5
29	2.965066129959747E-4	2.69551466359977E-5	0.0	2.246262219666475E-4	8.9850488786659E-5
30	3.0998418631397355E-4	2.69551466359977E-5	0.0	2.2911874640598045E-4	8.9850488786659E-5
31	3.459243818286371E-4	2.69551466359977E-5	0.0	2.2911874640598045E-4	8.9850488786659E-5
32	4.0881972397929843E-4	2.69551466359977E-5	0.0	2.2911874640598045E-4	9.434301322599194E-5
33	4.8519263944795857E-4	2.69551466359977E-5	0.0	2.336112708453134E-4	1.0332806210465785E-4
34	5.885207015526165E-4	2.69551466359977E-5	0.0	2.336112708453134E-4	1.0332806210465785E-4
35	6.738786658999425E-4	2.69551466359977E-5	0.0	2.3810379528464636E-4	1.0332806210465785E-4
36	7.18803910293272E-4	2.69551466359977E-5	0.0	2.4708884416331224E-4	1.0332806210465785E-4
37	7.906843013225992E-4	2.69551466359977E-5	0.0	2.5607389304197815E-4	1.078205865439908E-4
38	8.490871190339276E-4	2.69551466359977E-5	0.0	2.6505894192064406E-4	1.1231311098332375E-4
39	9.434301322599195E-4	2.69551466359977E-5	0.0	2.6505894192064406E-4	1.168056354226567E-4
40	0.0010063254744105809	2.69551466359977E-5	0.0	2.6505894192064406E-4	1.168056354226567E-4
41	0.0011321161587119035	2.69551466359977E-5	0.0	2.785365152386429E-4	1.2129815986198964E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	174215	0.0	44.16999	1
TCAACGC	149660	0.0	42.780804	12
CAACGCA	149775	0.0	42.733833	13
GGTATCA	178830	0.0	42.693707	8
GTATCAA	178950	0.0	42.662445	9
TGGTATC	179060	0.0	42.63624	7
GTGGTAT	179195	0.0	42.602806	6
AGTGGTA	179645	0.0	42.594196	5
AACGCAG	150690	0.0	42.444725	14
ACGCAGA	150600	0.0	42.42171	15
ATCAACG	151230	0.0	42.335117	11
AGAGTAC	178330	0.0	42.114983	19
CGCAGAG	152335	0.0	41.93701	16
AGCAGTG	184275	0.0	41.738247	2
GCAGTGG	184585	0.0	41.62868	3
CAGAGTA	181020	0.0	41.624157	18
GCAGAGT	182205	0.0	41.59979	17
CAGTGGT	184365	0.0	41.552166	4
GAGTACT	74250	0.0	41.51825	20
TATCAAC	153820	0.0	41.51534	10
>>END_MODULE