FastQCFastQC Report
Thu 26 May 2016
SRR805416_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805416_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20844327
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT349860.1678442292715903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT330200.024.3824961
AGAGTAC372850.020.8685819
GAGTACT141200.019.10623220
GTATCAA429750.018.5922059
AGTGGTA430950.018.5731535
GGTATCA429400.018.5581048
AGCAGTG435750.018.530352
TCAACGC369300.018.511112
CAACGCA372600.018.34715513
GTGGTAT436500.018.3316156
TGGTATC438500.018.2051327
GTACTTT149150.018.15085622
AACGCAG381700.017.94052914
GTACATG282550.017.93169422
ACGCAGA381800.017.88043615
GCAGAGT453250.017.62305517
GAGTACA292550.017.53563520
ATCAACG390450.017.52644311
GCAGTGG459900.017.5164173
CAGAGTA452800.017.45373318
Produced by FastQC (version 0.11.5)