##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805416_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20844327
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.77640914000246	37.0	35.0	37.0	35.0	37.0
2	35.902592441578946	37.0	35.0	37.0	35.0	37.0
3	37.730093996318516	39.0	38.0	39.0	35.0	39.0
4	37.760323324423	39.0	38.0	39.0	35.0	39.0
5	37.74608203949209	39.0	38.0	39.0	35.0	39.0
6	37.72370914158083	39.0	37.0	39.0	35.0	39.0
7	37.73551168142776	39.0	38.0	39.0	35.0	39.0
8	38.68379027060936	40.0	39.0	40.0	36.0	40.0
9	38.6765647075101	40.0	39.0	40.0	36.0	40.0
10	38.65119219248479	40.0	39.0	40.0	36.0	40.0
11	38.59812547557904	40.0	39.0	40.0	36.0	40.0
12	38.550751914417766	40.0	39.0	40.0	36.0	40.0
13	38.66058405243786	40.0	39.0	40.0	36.0	40.0
14	38.582397119369695	40.0	39.0	40.0	36.0	40.0
15	38.58157627252729	40.0	39.0	40.0	36.0	40.0
16	32.834772549864525	40.0	36.0	40.0	3.0	40.0
17	35.12600737841044	40.0	36.0	40.0	18.0	40.0
18	37.09669523031374	40.0	35.0	40.0	32.0	40.0
19	37.874293710706034	40.0	37.0	40.0	34.0	40.0
20	38.047124332678145	40.0	38.0	40.0	34.0	40.0
21	38.123012222942	40.0	38.0	40.0	34.0	40.0
22	38.184779484605095	40.0	38.0	40.0	34.0	40.0
23	38.118431072396824	40.0	38.0	40.0	34.0	40.0
24	38.09898866008003	40.0	38.0	40.0	34.0	40.0
25	38.08245236221827	40.0	38.0	40.0	34.0	40.0
26	38.04167570389776	40.0	38.0	40.0	34.0	40.0
27	37.98486206822604	40.0	38.0	40.0	34.0	40.0
28	37.86182139629646	40.0	38.0	40.0	34.0	40.0
29	37.654543272133466	40.0	38.0	40.0	33.0	40.0
30	37.586273521807634	40.0	38.0	40.0	33.0	40.0
31	37.68891339115914	40.0	38.0	40.0	33.0	40.0
32	37.73611328396451	40.0	38.0	40.0	33.0	40.0
33	37.755259740456	40.0	38.0	40.0	33.0	40.0
34	37.76563033193636	40.0	38.0	40.0	33.0	40.0
35	37.69316879360029	40.0	38.0	40.0	33.0	40.0
36	37.7951644588957	40.0	38.0	40.0	33.0	40.0
37	37.76568046548109	40.0	38.0	40.0	33.0	40.0
38	37.744614637834076	40.0	38.0	40.0	33.0	40.0
39	37.785855739069916	40.0	38.0	40.0	33.0	40.0
40	37.74843356660064	40.0	38.0	40.0	33.0	40.0
41	37.66428990487436	40.0	38.0	40.0	33.0	40.0
42	37.64879120347709	40.0	38.0	40.0	33.0	40.0
43	37.6238096341513	40.0	37.0	40.0	33.0	40.0
44	37.53129458197427	40.0	37.0	40.0	33.0	40.0
45	37.44464194022671	40.0	37.0	40.0	33.0	40.0
46	37.336362071080536	40.0	37.0	40.0	32.0	40.0
47	37.09813355931328	40.0	37.0	40.0	32.0	40.0
48	37.10454249734232	40.0	37.0	40.0	32.0	40.0
49	37.05438347805617	40.0	36.0	40.0	31.0	40.0
50	37.01758353723773	40.0	36.0	40.0	31.0	40.0
51	36.941935184570845	39.0	36.0	40.0	31.0	40.0
52	36.84124529422322	39.0	36.0	40.0	31.0	40.0
53	36.30406277928762	39.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	14.0
11	27.0
12	61.0
13	122.0
14	336.0
15	656.0
16	1363.0
17	2561.0
18	4570.0
19	7993.0
20	13084.0
21	20602.0
22	29882.0
23	41667.0
24	57082.0
25	75542.0
26	98393.0
27	126030.0
28	159941.0
29	200406.0
30	250985.0
31	312043.0
32	388466.0
33	489748.0
34	635874.0
35	872564.0
36	1297710.0
37	2330307.0
38	4245745.0
39	9180551.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.661953681689987	28.628595204824798	22.852342510266702	24.857108603218517
2	25.45006130444989	23.70034302378772	28.632126141563603	22.21746953019879
3	21.421790206994928	25.443752633510307	27.113050951465116	26.021406208029646
4	22.269742745831994	28.2608884422126	26.393291565613993	23.076077246341413
5	25.52966090006168	27.562588132492838	24.988314566356593	21.91943640108889
6	24.59050848703343	25.72110867383725	27.104924040003787	22.583458799125538
7	23.92828513964495	26.557345794853436	26.966766545161185	22.547602520340426
8	23.51103492091637	25.717294686463134	27.113631445140925	23.65803894747957
9	23.189335880213356	25.748214370269668	27.76945976715871	23.29298998235827
10	22.764798306992592	26.492450439872684	27.633614652082557	23.10913660105217
11	22.772949205795896	27.088358381635448	26.970556545193325	23.16813586737533
12	22.838473029136416	26.195669450013902	27.377002865096102	23.58885465575358
13	22.988130055722117	26.608582757313297	26.34779717282309	24.055490014141498
14	23.26600902010413	27.143562850458064	26.346060489264055	23.244367640173753
15	23.170784069929436	26.75411875854759	26.299539438236604	23.775557733286377
16	19.76985392716205	37.97817506892883	21.98128536363875	20.270685640270372
17	24.276211940064076	26.086704550355595	26.17844653847543	23.458636971104895
18	23.34476426127838	26.253785982152362	26.137984690031008	24.26346506653825
19	23.81202808802606	26.87946221530683	26.036561410689824	23.271948285977285
20	24.25601459812063	26.147325360996305	26.28304094442579	23.313619096457273
21	23.622590453508046	26.72296879625809	26.265117602501633	23.389323147732235
22	24.201817597660984	26.0241071827361	26.345139375332195	23.428935844270722
23	23.370718565295967	26.248969323883664	27.001653735330482	23.378658375489888
24	23.356666780366666	26.704244277111943	26.20739446277157	23.731694479749816
25	23.544286174362934	26.09687998082164	26.248595121348846	24.11023872346658
26	23.66391584626359	26.315308716851355	26.791726113296914	23.22904932358814
27	23.811533948781364	25.724087901710618	27.02680206465769	23.437576084850328
28	24.427178675521642	25.893664017072847	26.34347465379909	23.335682653606423
29	24.47106591640018	25.837831079890467	26.480447173948097	23.210655829761258
30	24.11681605263629	25.832016548195586	26.461655490244418	23.589511908923708
31	23.98207435529101	26.099456221349822	26.372844755314002	23.54562466804517
32	23.826103860297337	26.132438816566257	26.52232427556908	23.51913304756733
33	23.716548872026426	25.995686979963423	26.53742670607691	23.75033744193324
34	23.84515460729435	26.10541947456495	26.406786844209456	23.642639073931242
35	23.80811815128404	26.054278461472997	26.53938407318212	23.59821931406085
36	23.74540564442306	25.992736536900424	26.479780325841173	23.78207749283534
37	23.926706772542953	26.121294297484393	26.306313463610508	23.645685466362142
38	23.81925307542911	26.128538474761022	26.502266060209088	23.54994238960078
39	23.790348328348525	25.998455119227405	26.47500204731964	23.736194505104436
40	23.879442113914255	26.123884930417756	26.336782185387897	23.659890770280086
41	23.81154354371815	26.112692436651948	26.508363642539283	23.56740037709061
42	23.78259082195362	25.96906582783891	26.50849797165435	23.739845378553117
43	23.8681968479961	26.1027137023901	26.31159547631353	23.717493973300265
44	23.845974974389915	26.030233549876662	26.48361830055727	23.64017317517615
45	23.7855556574218	25.878374485297606	26.50631992100297	23.829749936277626
46	23.89228013933959	26.041406853768894	26.33833656514792	23.727976441743596
47	23.766778366123308	26.300268653432656	26.352263615898945	23.580689364545087
48	23.81449398678115	25.91490720712643	26.43761057864809	23.83298822744433
49	23.886499189923473	26.104560727722227	26.290184374866122	23.71875570748818
50	23.869439392310436	26.0563365754145	26.4379943761197	23.636229656155365
51	23.859307139059947	25.95005346058906	26.383782983254868	23.80685641709612
52	23.907799949597795	26.078174651549073	26.253646855568903	23.760378543284222
53	23.87116168346428	26.12144301900464	26.330507096727086	23.67688820080399
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7568.0
1	4755.0
2	1942.0
3	1707.5
4	1473.0
5	1568.5
6	1664.0
7	1888.0
8	2530.0
9	2948.0
10	3890.0
11	4832.0
12	7005.5
13	9179.0
14	11998.5
15	14818.0
16	21353.5
17	27889.0
18	38237.5
19	48586.0
20	71364.0
21	94142.0
22	138452.5
23	182763.0
24	187091.5
25	225883.5
26	260347.0
27	297536.0
28	334725.0
29	376596.5
30	418468.0
31	456548.0
32	494628.0
33	548770.0
34	602912.0
35	668625.0
36	734338.0
37	802420.0
38	870502.0
39	936017.5
40	1001533.0
41	1069360.5
42	1198856.5
43	1260525.0
44	1319191.5
45	1377858.0
46	1425321.0
47	1472784.0
48	1485616.0
49	1498448.0
50	1501847.5
51	1505247.0
52	1479513.5
53	1453780.0
54	1399516.0
55	1345252.0
56	1261669.0
57	1178086.0
58	1080523.5
59	873627.0
60	764293.0
61	670378.5
62	576464.0
63	488566.5
64	400669.0
65	328371.0
66	256073.0
67	205825.0
68	155577.0
69	121194.5
70	86812.0
71	64948.5
72	43085.0
73	31774.0
74	20463.0
75	15451.5
76	7794.5
77	5149.0
78	3695.5
79	2242.0
80	1722.0
81	1202.0
82	891.5
83	581.0
84	382.0
85	183.0
86	128.0
87	73.0
88	64.5
89	56.0
90	40.5
91	25.0
92	20.0
93	9.5
94	4.0
95	2.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.0844327E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.677450470728374
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.15659588848582	37.48348055581345
2	16.83481564083832	17.736303342082078
3	5.135184504832655	8.115252822341219
4	2.202489685165217	4.640861652103234
5	1.1638178281904368	3.0653478000726193
6	0.6981972161014754	2.206754956199154
7	0.47471827750301365	1.7504863985500139
8	0.32179110114278375	1.356090783389611
9	0.26107556944080507	1.2377515840501694
>10	1.6271071534357227	15.947826748549332
>50	0.09606190802890933	3.440759986361202
>100	0.02701390820143869	2.3193522912160933
>500	9.84680801281882E-4	0.34815076092870273
>1k	1.1918650547619896E-4	0.10337274716240856
>5k	9.150442212681482E-6	0.030680698164147292
>10k+	1.830088442536266E-5	0.2175268730167503
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34986	0.1678442292715903	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8230379901447525E-4	0.0	4.79746839511777E-6	4.79746839511777E-6	4.79746839511777E-6
2	1.8230379901447525E-4	0.0	4.79746839511777E-6	4.79746839511777E-6	9.59493679023554E-6
3	1.8230379901447525E-4	0.0	4.79746839511777E-6	1.439240518535331E-5	1.439240518535331E-5
4	1.8230379901447525E-4	0.0	4.79746839511777E-6	1.439240518535331E-5	1.918987358047108E-5
5	1.8230379901447525E-4	0.0	9.59493679023554E-6	8.15569627170021E-5	1.918987358047108E-5
6	1.8230379901447525E-4	0.0	9.59493679023554E-6	8.15569627170021E-5	2.398734197558885E-5
7	1.8230379901447525E-4	0.0	9.59493679023554E-6	1.3912658345841534E-4	2.878481037070662E-5
8	1.8230379901447525E-4	0.0	9.59493679023554E-6	1.3912658345841534E-4	3.837974716094216E-5
9	1.8230379901447525E-4	0.0	1.439240518535331E-5	1.439240518535331E-4	3.837974716094216E-5
10	1.8230379901447525E-4	0.0	1.439240518535331E-5	1.4872152024865087E-4	3.837974716094216E-5
11	1.8710126740959301E-4	0.0	1.439240518535331E-5	1.4872152024865087E-4	3.837974716094216E-5
12	1.9669620419982857E-4	0.0	1.439240518535331E-5	1.4872152024865087E-4	3.837974716094216E-5
13	2.2548101457053519E-4	4.79746839511777E-6	1.918987358047108E-5	1.4872152024865087E-4	4.317721555605993E-5
14	2.6386076173147736E-4	4.79746839511777E-6	1.918987358047108E-5	1.4872152024865087E-4	5.2772152346295466E-5
15	3.1183544568265506E-4	4.79746839511777E-6	1.918987358047108E-5	1.5351898864376863E-4	5.2772152346295466E-5
16	3.5981012963383276E-4	4.79746839511777E-6	1.918987358047108E-5	1.5351898864376863E-4	6.236708913653101E-5
17	3.837974716094216E-4	4.79746839511777E-6	1.918987358047108E-5	1.5351898864376863E-4	6.236708913653101E-5
18	4.317721555605993E-4	4.79746839511777E-6	1.918987358047108E-5	1.5351898864376863E-4	6.236708913653101E-5
19	4.653544343264237E-4	4.79746839511777E-6	1.918987358047108E-5	1.5351898864376863E-4	7.675949432188432E-5
20	5.708987390190146E-4	4.79746839511777E-6	2.398734197558885E-5	1.583164570388864E-4	7.675949432188432E-5
21	6.188734229701923E-4	4.79746839511777E-6	2.398734197558885E-5	1.583164570388864E-4	8.15569627170021E-5
22	7.148227908725477E-4	4.79746839511777E-6	2.398734197558885E-5	1.583164570388864E-4	9.115189950723762E-5
23	8.395569691456098E-4	4.79746839511777E-6	2.398734197558885E-5	1.631139254340042E-4	1.0074683629747317E-4
24	8.97126589887023E-4	4.79746839511777E-6	2.398734197558885E-5	1.6791139382912196E-4	1.0074683629747317E-4
25	0.001041050641740556	4.79746839511777E-6	2.398734197558885E-5	1.6791139382912196E-4	1.0074683629747317E-4
26	0.0011561898832233826	4.79746839511777E-6	2.398734197558885E-5	1.6791139382912196E-4	1.0074683629747317E-4
27	0.001271329124706209	4.79746839511777E-6	2.398734197558885E-5	1.7270886222423972E-4	1.0554430469259093E-4
28	0.001458430392115802	4.79746839511777E-6	3.358227876582439E-5	1.7270886222423972E-4	1.1034177308770871E-4
29	0.0015831645703888641	4.79746839511777E-6	3.358227876582439E-5	1.7270886222423972E-4	1.1993670987794425E-4
30	0.0017270886222423973	9.59493679023554E-6	3.358227876582439E-5	1.7270886222423972E-4	1.1993670987794425E-4
31	0.001895000016071519	9.59493679023554E-6	3.358227876582439E-5	1.7750633061935748E-4	1.1993670987794425E-4
32	0.002058113941505523	9.59493679023554E-6	3.358227876582439E-5	1.7750633061935748E-4	1.3432911506329755E-4
33	0.0023651519187930605	9.59493679023554E-6	3.358227876582439E-5	1.7750633061935748E-4	1.439240518535331E-4
34	0.0026338101489196556	9.59493679023554E-6	3.358227876582439E-5	1.7750633061935748E-4	1.439240518535331E-4
35	0.0029216582526267218	9.59493679023554E-6	3.358227876582439E-5	1.7750633061935748E-4	1.5351898864376863E-4
36	0.003171126609172846	9.59493679023554E-6	4.317721555605993E-5	1.8710126740959301E-4	1.583164570388864E-4
37	0.003444582307694559	9.59493679023554E-6	4.317721555605993E-5	1.918987358047108E-4	1.583164570388864E-4
38	0.003775607626957685	9.59493679023554E-6	5.2772152346295466E-5	1.918987358047108E-4	1.583164570388864E-4
39	0.004082645604245223	9.59493679023554E-6	5.2772152346295466E-5	1.9669620419982857E-4	1.583164570388864E-4
40	0.004519215228200939	9.59493679023554E-6	5.2772152346295466E-5	1.9669620419982857E-4	1.631139254340042E-4
41	0.004960582320551774	9.59493679023554E-6	5.2772152346295466E-5	2.0149367259494634E-4	1.7270886222423972E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	33020	0.0	24.382496	1
AGAGTAC	37285	0.0	20.86858	19
GAGTACT	14120	0.0	19.106232	20
GTATCAA	42975	0.0	18.592205	9
AGTGGTA	43095	0.0	18.573153	5
GGTATCA	42940	0.0	18.558104	8
AGCAGTG	43575	0.0	18.53035	2
TCAACGC	36930	0.0	18.5111	12
CAACGCA	37260	0.0	18.347155	13
GTGGTAT	43650	0.0	18.331615	6
TGGTATC	43850	0.0	18.205132	7
GTACTTT	14915	0.0	18.150856	22
AACGCAG	38170	0.0	17.940529	14
GTACATG	28255	0.0	17.931694	22
ACGCAGA	38180	0.0	17.880436	15
GCAGAGT	45325	0.0	17.623055	17
GAGTACA	29255	0.0	17.535635	20
ATCAACG	39045	0.0	17.526443	11
GCAGTGG	45990	0.0	17.516417	3
CAGAGTA	45280	0.0	17.453733	18
>>END_MODULE