FastQCFastQC Report
Thu 26 May 2016
SRR805415_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805415_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19502961
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1209060.619936634237232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT628750.040.388231
TCAACGC584200.036.54929412
CAACGCA585750.036.47263713
GTATCAA694700.036.2969639
GGTATCA695300.036.25558
AACGCAG592750.036.022114
TGGTATC702750.035.86787
ACGCAGA594600.035.86654715
AGTGGTA706000.035.8158655
GTGGTAT705600.035.7195976
ATCAACG602350.035.41678211
AGAGTAC709700.035.2119219
CGCAGAG609800.034.991816
AGCAGTG730100.034.8685762
CAGAGTA728800.034.41808318
GCAGTGG743000.034.2283973
GCAGAGT735050.034.22454517
CAGTGGT746450.033.9568634
TATCAAC626900.033.95485710
GAGTACT286500.033.7448520
Produced by FastQC (version 0.11.5)