##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805415_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19502961
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.1389478243842	37.0	35.0	37.0	35.0	37.0
2	36.14346918911441	37.0	35.0	37.0	35.0	37.0
3	37.90696669085274	39.0	38.0	39.0	35.0	39.0
4	37.87046433615901	39.0	38.0	39.0	35.0	39.0
5	37.82516998316307	39.0	38.0	39.0	35.0	39.0
6	37.79486658461759	39.0	38.0	39.0	35.0	39.0
7	37.77595320013202	39.0	38.0	39.0	35.0	39.0
8	38.737869290719495	40.0	39.0	40.0	36.0	40.0
9	38.73290122458841	40.0	39.0	40.0	36.0	40.0
10	38.6590574631206	40.0	39.0	40.0	36.0	40.0
11	38.64556238409132	40.0	39.0	40.0	36.0	40.0
12	38.61435266162918	40.0	39.0	40.0	36.0	40.0
13	38.73321112624898	40.0	39.0	40.0	36.0	40.0
14	38.658462938012335	40.0	39.0	40.0	36.0	40.0
15	38.66050570474914	40.0	39.0	40.0	36.0	40.0
16	32.90532945228163	40.0	36.0	40.0	3.0	40.0
17	35.175387573199785	40.0	36.0	40.0	18.0	40.0
18	37.172426484368195	40.0	36.0	40.0	32.0	40.0
19	37.95225663426184	40.0	37.0	40.0	34.0	40.0
20	38.1541636164888	40.0	38.0	40.0	34.0	40.0
21	38.2053431784025	40.0	38.0	40.0	34.0	40.0
22	38.27994774742153	40.0	38.0	40.0	35.0	40.0
23	38.14220358641952	40.0	38.0	40.0	34.0	40.0
24	38.17312535260672	40.0	38.0	40.0	34.0	40.0
25	38.174658248047564	40.0	38.0	40.0	34.0	40.0
26	38.14781627261625	40.0	38.0	40.0	34.0	40.0
27	38.10145690185198	40.0	38.0	40.0	34.0	40.0
28	37.99434511508278	40.0	38.0	40.0	34.0	40.0
29	37.801585769463415	40.0	38.0	40.0	33.0	40.0
30	37.72651660432485	40.0	38.0	40.0	33.0	40.0
31	37.80807950136392	40.0	38.0	40.0	33.0	40.0
32	37.860918606154215	40.0	38.0	40.0	33.0	40.0
33	37.86367080362823	40.0	38.0	40.0	33.0	40.0
34	37.87354750901671	40.0	38.0	40.0	33.0	40.0
35	37.806651615618776	40.0	38.0	40.0	33.0	40.0
36	37.86501003616836	40.0	38.0	40.0	33.0	40.0
37	37.82379198727824	40.0	38.0	40.0	33.0	40.0
38	37.81868312201414	40.0	38.0	40.0	33.0	40.0
39	37.84152467925255	40.0	38.0	40.0	33.0	40.0
40	37.79986069807554	40.0	38.0	40.0	33.0	40.0
41	37.733521079183824	40.0	38.0	40.0	33.0	40.0
42	37.71698964070122	40.0	38.0	40.0	33.0	40.0
43	37.68482155094296	40.0	37.0	40.0	33.0	40.0
44	37.60118855798358	40.0	37.0	40.0	33.0	40.0
45	37.50716847559712	40.0	37.0	40.0	33.0	40.0
46	37.39762234052563	40.0	37.0	40.0	33.0	40.0
47	37.1778957051701	40.0	37.0	40.0	32.0	40.0
48	37.15731590705637	40.0	36.0	40.0	32.0	40.0
49	37.10338009700168	40.0	36.0	40.0	32.0	40.0
50	37.05379413925916	40.0	36.0	40.0	32.0	40.0
51	36.95757336539821	39.0	36.0	40.0	32.0	40.0
52	36.8216396474361	39.0	36.0	40.0	31.0	40.0
53	36.3217087907831	39.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	5.0
12	10.0
13	45.0
14	101.0
15	347.0
16	766.0
17	1726.0
18	3328.0
19	6031.0
20	10258.0
21	16771.0
22	24958.0
23	35755.0
24	49789.0
25	65903.0
26	87308.0
27	111383.0
28	142466.0
29	178807.0
30	224028.0
31	277768.0
32	346659.0
33	437543.0
34	570274.0
35	788129.0
36	1187271.0
37	2177209.0
38	3909850.0
39	8848472.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.11198822578787	32.91812458631282	24.038585730648798	24.93130145725052
2	26.6546500298083	27.613586470280076	25.723094047103924	20.008669452807702
3	23.75698233719485	25.203280671073486	26.16320670486907	24.876530286862593
4	21.026094447914858	28.41056288837372	25.186267869786544	25.37707479392488
5	24.350722949197305	29.63304392599667	24.85221090274446	21.16402222206156
6	25.954771688258006	25.658088533325785	26.32438223098534	22.062757547430873
7	23.547086004017544	26.400175850220897	28.411367894341787	21.641370251419772
8	24.50858615776343	25.709460219912252	26.86695112603671	22.915002496287613
9	24.434694813777252	25.37721323444168	27.622431281075734	22.565660670705334
10	22.138438363282376	26.411517717745525	29.023931289202704	22.4261126297694
11	22.096588307795926	28.916803966331063	26.618260683595686	22.36834704227732
12	22.180847308262578	26.04782935268137	28.898217045093823	22.873106293962234
13	22.27124896573397	26.49233108757178	25.965939223279992	25.270480723414256
14	22.64847373688539	29.0215162712985	25.876481012293468	22.45352897952265
15	22.497512044453146	28.634933946696606	25.816725983300685	23.050828025549556
16	19.264285048818998	37.860440781274185	21.580210307552786	21.29506386235403
17	25.658652550246085	25.802287150140945	25.722545412463266	22.8165148871497
18	22.723795632878517	26.00757905427796	25.685145963220663	25.583479349622863
19	23.11636166426216	28.635103151772697	25.6109162090823	22.63761897488284
20	25.64451110782614	25.99081236946533	25.780382783927013	22.58429373878151
21	23.134938330646303	28.427129603550966	25.755550657154057	22.682381408648666
22	25.49715399625729	25.81582868365475	25.989443346576962	22.697573973510995
23	22.81390502703666	26.020213033292737	28.585736288966583	22.580145650704015
24	22.735763046442024	28.374681157389382	25.80412789627175	23.08542789989684
25	22.74194159543261	26.014829235417125	25.8235557154629	25.419673453687363
26	22.945131254684867	27.326568514391226	27.19925964062585	22.52904059029806
27	23.118771554739816	25.520176141458723	28.613608979682624	22.747443324118834
28	24.789046135097127	25.74150150841198	26.789362907509272	22.68008944898162
29	25.033732057404002	25.621463325491963	26.83025926165776	22.514545355446284
30	24.659101764085978	25.62029427223897	26.872586167813182	22.848017795861868
31	24.01992702543988	26.077870944827303	26.913943990351	22.988258039381815
32	23.687577491438354	26.251301020393775	27.059086053651033	23.00203543451684
33	23.548388370360787	26.01949006614944	27.12023574266492	23.31188582082485
34	23.46969775512549	26.155956523729913	27.10884772830136	23.26549799284324
35	23.475548148816994	26.115865175549498	27.254794797569453	23.15379187806405
36	23.413029436914734	26.042163546345602	27.216005815732284	23.328801201007376
37	23.59867816994558	26.199831912702898	27.032987452520672	23.168502464830855
38	23.630611782487797	26.16719071529703	27.1544202954618	23.047777206753377
39	23.504302756899325	26.061704168920812	27.196398536611955	23.23759453756791
40	23.485397935216092	26.2546543573563	27.045462481312455	23.21448522611515
41	23.540543407742035	26.227627692020718	27.166869687120844	23.06495921311641
42	23.484003275194983	26.09155091885791	27.189738009525836	23.234707796421272
43	23.49702693862742	26.230314463531972	27.095757408323795	23.176901189516812
44	23.561965795860434	26.093709565434704	27.20615090190664	23.138173736798223
45	23.53019113354121	25.912039715405268	27.21844647076923	23.33932268028429
46	23.521997505917177	26.119239022218217	27.051969185602125	23.30679428626248
47	23.465913714332917	26.332642515154493	27.038053349950296	23.163390420562294
48	23.453382283849102	25.982182910584704	27.160347600551525	23.404087205014665
49	23.454802581002955	26.249870468386828	26.97311962014383	23.322207330466384
50	23.584721314881367	26.184962375713106	27.05047197705005	23.179844332355483
51	23.553187641609906	26.051351894719986	27.06757194458831	23.327888519081792
52	23.608081870234987	26.198980759895896	26.8862456321376	23.306691737731516
53	23.69817588211349	26.239333606830268	26.839124582159602	23.223365928896644
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6594.0
1	4001.5
2	1409.0
3	1204.0
4	999.0
5	1071.5
6	1144.0
7	1318.0
8	1854.5
9	2217.0
10	3318.0
11	4419.0
12	6447.5
13	8476.0
14	11333.0
15	14190.0
16	20391.5
17	26593.0
18	36960.0
19	47327.0
20	78949.5
21	110572.0
22	195584.5
23	280597.0
24	244588.0
25	237424.0
26	266269.0
27	302520.5
28	338772.0
29	379698.5
30	420625.0
31	458312.5
32	496000.0
33	547151.0
34	598302.0
35	663086.5
36	727871.0
37	794995.5
38	862120.0
39	921761.0
40	981402.0
41	1039562.0
42	1148840.0
43	1199958.0
44	1241806.5
45	1283655.0
46	1312130.0
47	1340605.0
48	1353567.5
49	1366530.0
50	1364817.0
51	1363104.0
52	1338912.5
53	1314721.0
54	1262772.5
55	1210824.0
56	1136038.0
57	1061252.0
58	966262.0
59	769514.5
60	667757.0
61	580206.5
62	492656.0
63	415047.5
64	337439.0
65	275888.0
66	214337.0
67	171337.0
68	128337.0
69	99714.5
70	71092.0
71	53923.5
72	36755.0
73	27840.5
74	18926.0
75	14520.0
76	7655.5
77	5197.0
78	3829.0
79	2461.0
80	1881.5
81	1302.0
82	964.5
83	627.0
84	409.0
85	191.0
86	126.5
87	62.0
88	55.0
89	48.0
90	38.0
91	28.0
92	20.5
93	9.0
94	5.0
95	3.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.9502961E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.71360489626019
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.07457373509023	39.78812386465528
2	14.353607773446047	15.419680335575398
3	4.65307347532932	7.49800050621322
4	2.101285008802505	4.514703709490095
5	1.1379916437035833	3.056281676256999
6	0.7437086348044214	2.396836306069305
7	0.5037475633434653	1.894066830941929
8	0.36007418646229583	1.5472706067982462
9	0.28432755346570376	1.3745032081179396
>10	1.6657287258614473	16.086330809497195
>50	0.09603207549279452	3.5433809427019054
>100	0.025488423994002897	1.8678507517994931
>500	1.8829277203451873E-4	0.06992546161304251
>1k	1.1529905139617327E-4	0.13507311166452574
>5k	3.8405587167901664E-5	0.12896750922844882
>10k+	1.9202793583943134E-5	0.6790043693770558
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	120906	0.619936634237232	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.229367786768378E-5	5.1274265482046546E-6	0.0	0.0	5.1274265482046546E-6
2	9.229367786768378E-5	5.1274265482046546E-6	0.0	0.0	1.0254853096409309E-5
3	9.229367786768378E-5	5.1274265482046546E-6	0.0	1.0254853096409309E-5	1.0254853096409309E-5
4	9.229367786768378E-5	5.1274265482046546E-6	0.0	1.0254853096409309E-5	2.0509706192818618E-5
5	9.229367786768378E-5	5.1274265482046546E-6	0.0	6.665654512666051E-5	2.5637132741023274E-5
6	9.229367786768378E-5	5.1274265482046546E-6	0.0	6.665654512666051E-5	3.589198583743258E-5
7	9.229367786768378E-5	5.1274265482046546E-6	0.0	1.9484220883177688E-4	3.589198583743258E-5
8	9.229367786768378E-5	5.1274265482046546E-6	0.0	2.050970619281862E-4	4.1019412385637237E-5
9	9.229367786768378E-5	5.1274265482046546E-6	0.0	2.050970619281862E-4	4.1019412385637237E-5
10	9.229367786768378E-5	5.1274265482046546E-6	0.0	2.050970619281862E-4	5.64016920302512E-5
11	9.229367786768378E-5	5.1274265482046546E-6	0.0	2.050970619281862E-4	6.152911857845585E-5
12	1.025485309640931E-4	1.5382279644613963E-5	0.0	2.1022448847639083E-4	8.203882477127447E-5
13	1.025485309640931E-4	1.5382279644613963E-5	0.0	2.1022448847639083E-4	9.742110441588844E-5
14	1.128033840605024E-4	2.0509706192818618E-5	0.0	2.2047934157280014E-4	1.4356794334973032E-4
15	1.128033840605024E-4	2.5637132741023274E-5	0.0	2.2047934157280014E-4	1.5382279644613964E-4
16	1.3844051680152568E-4	2.5637132741023274E-5	0.0	2.2047934157280014E-4	1.6407764954254895E-4
17	1.589502229943443E-4	3.0764559289227926E-5	0.0	2.256067681210048E-4	1.7433250263895826E-4
18	1.7945992918716293E-4	3.0764559289227926E-5	0.0	2.4098904776561876E-4	1.7433250263895826E-4
19	1.9996963537998152E-4	3.589198583743258E-5	0.0	2.4098904776561876E-4	1.897147822835722E-4
20	2.050970619281862E-4	4.1019412385637237E-5	0.0	2.512439008620281E-4	2.050970619281862E-4
21	2.256067681210048E-4	4.1019412385637237E-5	0.0	2.512439008620281E-4	2.153519150245955E-4
22	2.461164743138234E-4	4.1019412385637237E-5	0.0	2.614987539584374E-4	2.3586162121741412E-4
23	2.6662618050664203E-4	4.1019412385637237E-5	0.0	2.717536070548467E-4	2.512439008620281E-4
24	2.9739073979587E-4	4.1019412385637237E-5	0.0	2.7688103360305137E-4	2.82008460151256E-4
25	3.281552990850979E-4	4.1019412385637237E-5	0.0	2.7688103360305137E-4	2.8713588669946065E-4
26	3.4353757872971187E-4	4.1019412385637237E-5	0.0	2.7688103360305137E-4	2.9739073979587E-4
27	3.794295645671444E-4	4.1019412385637237E-5	0.0	2.7688103360305137E-4	2.9739073979587E-4
28	4.2557640350098636E-4	4.1019412385637237E-5	0.0	2.7688103360305137E-4	3.127730194404839E-4
29	4.768506689830329E-4	4.614683893384189E-5	0.0	2.82008460151256E-4	3.179004459886886E-4
30	5.281249344650795E-4	5.127426548204655E-5	0.0	2.8713588669946065E-4	3.3841015218150723E-4
31	6.152911857845585E-4	5.64016920302512E-5	0.0	2.8713588669946065E-4	3.4353757872971187E-4
32	6.511831716219911E-4	5.64016920302512E-5	0.0	2.922633132476653E-4	3.640472849225305E-4
33	7.691139822306982E-4	5.64016920302512E-5	0.0	2.922633132476653E-4	3.948118442117584E-4
34	8.61407660098382E-4	6.152911857845585E-5	0.0	3.0251816634407463E-4	3.9993927075996304E-4
35	9.793384707070891E-4	6.152911857845585E-5	0.0	3.0764559289227927E-4	4.409586831456003E-4
36	0.0010767595751229775	6.152911857845585E-5	0.0	3.0764559289227927E-4	4.8710552207944217E-4
37	0.0011639258264424567	6.152911857845585E-5	0.0	3.0764559289227927E-4	5.281249344650795E-4
38	0.0012869840635993683	6.152911857845585E-5	0.0	3.0764559289227927E-4	5.383797875614888E-4
39	0.0014151697273044846	6.665654512666051E-5	0.0	3.127730194404839E-4	5.48634640657898E-4
40	0.001558737670654215	7.178397167486516E-5	0.0	3.2302787253689325E-4	5.64016920302512E-4
41	0.0017587073060341964	7.178397167486516E-5	0.0	3.281552990850979E-4	5.79399199947126E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	62875	0.0	40.38823	1
TCAACGC	58420	0.0	36.549294	12
CAACGCA	58575	0.0	36.472637	13
GTATCAA	69470	0.0	36.296963	9
GGTATCA	69530	0.0	36.2555	8
AACGCAG	59275	0.0	36.0221	14
TGGTATC	70275	0.0	35.8678	7
ACGCAGA	59460	0.0	35.866547	15
AGTGGTA	70600	0.0	35.815865	5
GTGGTAT	70560	0.0	35.719597	6
ATCAACG	60235	0.0	35.416782	11
AGAGTAC	70970	0.0	35.21192	19
CGCAGAG	60980	0.0	34.9918	16
AGCAGTG	73010	0.0	34.868576	2
CAGAGTA	72880	0.0	34.418083	18
GCAGTGG	74300	0.0	34.228397	3
GCAGAGT	73505	0.0	34.224545	17
CAGTGGT	74645	0.0	33.956863	4
TATCAAC	62690	0.0	33.954857	10
GAGTACT	28650	0.0	33.74485	20
>>END_MODULE