FastQCFastQC Report
Thu 26 May 2016
SRR805414_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805414_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20896146
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4606672.2045548494923417No Hit
AAGCAGTGGTATCAAAGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT881310.42175719867194644No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG604190.2891394422684451No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG370950.17752077344788844No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC247820.1185960320147074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2435600.044.380421
GTGGTAT2478700.043.353696
AGTGGTA2487900.043.2868845
TGGTATC2482550.043.276057
GTATCAA2484550.043.2535069
GGTATCA2485150.043.239288
AGAGTAC2448650.043.1534719
TCAACGC2103200.043.07476412
CAACGCA2103950.043.06387713
AGCAGTG2524300.042.8907852
AACGCAG2114700.042.84496714
ACGCAGA2112350.042.8436815
GCAGTGG2529950.042.749483
ATCAACG2121300.042.72052411
CGCAGAG2124400.042.59402516
GCAGAGT2511350.042.51026517
CAGAGTA2500250.042.49785218
CAGTGGT2536650.042.495754
GAGTACT875100.042.48314320
GTACTTT870800.042.4203622