##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805414_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20896146
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.25051796632738	37.0	36.0	37.0	35.0	37.0
2	36.21903886008453	37.0	36.0	37.0	35.0	37.0
3	37.98665548182904	39.0	38.0	39.0	35.0	39.0
4	37.92714359863297	39.0	38.0	39.0	35.0	39.0
5	37.84818071236677	39.0	38.0	39.0	35.0	39.0
6	37.85485313894725	39.0	38.0	39.0	35.0	39.0
7	37.73909767858628	39.0	37.0	39.0	35.0	39.0
8	38.748890872029705	40.0	39.0	40.0	36.0	40.0
9	38.78039280544843	40.0	39.0	40.0	36.0	40.0
10	38.45045454793434	40.0	38.0	40.0	36.0	40.0
11	38.59511117504635	40.0	39.0	40.0	36.0	40.0
12	38.636659506494645	40.0	39.0	40.0	36.0	40.0
13	38.78851219741669	40.0	39.0	40.0	36.0	40.0
14	38.74618597132696	40.0	39.0	40.0	36.0	40.0
15	38.73997171535842	40.0	39.0	40.0	36.0	40.0
16	32.888845579467144	40.0	36.0	40.0	3.0	40.0
17	35.2072699434623	40.0	36.0	40.0	18.0	40.0
18	37.22168786531258	40.0	36.0	40.0	32.0	40.0
19	37.98669343141075	40.0	37.0	40.0	34.0	40.0
20	38.229746624090396	40.0	38.0	40.0	35.0	40.0
21	38.19571546829736	40.0	38.0	40.0	34.0	40.0
22	38.35388784132729	40.0	38.0	40.0	35.0	40.0
23	37.84949277249498	40.0	38.0	40.0	34.0	40.0
24	38.090257696323526	40.0	38.0	40.0	34.0	40.0
25	38.1933819279402	40.0	38.0	40.0	34.0	40.0
26	38.19179354891567	40.0	38.0	40.0	34.0	40.0
27	38.19171004069363	40.0	38.0	40.0	34.0	40.0
28	38.09025506425922	40.0	38.0	40.0	34.0	40.0
29	37.90126026110269	40.0	38.0	40.0	34.0	40.0
30	37.82117156914964	40.0	38.0	40.0	33.0	40.0
31	37.85752554561975	40.0	38.0	40.0	33.0	40.0
32	37.910322314937886	40.0	38.0	40.0	34.0	40.0
33	37.853333863574655	40.0	38.0	40.0	33.0	40.0
34	37.87565573096589	40.0	38.0	40.0	33.0	40.0
35	37.81339530265533	40.0	38.0	40.0	33.0	40.0
36	37.8747341256134	40.0	38.0	40.0	33.0	40.0
37	37.77540882419179	40.0	37.0	40.0	33.0	40.0
38	37.696143394097646	40.0	37.0	40.0	33.0	40.0
39	37.71760074800396	40.0	37.0	40.0	33.0	40.0
40	37.68276188345928	40.0	37.0	40.0	33.0	40.0
41	37.60872713083073	40.0	37.0	40.0	33.0	40.0
42	37.59450843232049	40.0	37.0	40.0	33.0	40.0
43	37.56556587037629	40.0	37.0	40.0	33.0	40.0
44	37.48470885492473	40.0	37.0	40.0	33.0	40.0
45	37.393906751991494	40.0	37.0	40.0	33.0	40.0
46	37.286390705731094	40.0	36.0	40.0	33.0	40.0
47	37.067981770418335	40.0	36.0	40.0	32.0	40.0
48	37.04255913985287	40.0	36.0	40.0	32.0	40.0
49	36.97634496811039	40.0	36.0	40.0	32.0	40.0
50	36.90238869885385	40.0	35.0	40.0	32.0	40.0
51	36.770025391285074	39.0	35.0	40.0	31.0	40.0
52	36.56668636407881	39.0	35.0	40.0	31.0	40.0
53	36.024313670090166	39.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	5.0
13	26.0
14	99.0
15	270.0
16	655.0
17	1348.0
18	2804.0
19	5017.0
20	8955.0
21	14668.0
22	22795.0
23	33238.0
24	47213.0
25	63797.0
26	85754.0
27	111412.0
28	143449.0
29	183174.0
30	230365.0
31	291114.0
32	369725.0
33	480672.0
34	644836.0
35	922177.0
36	1430468.0
37	2590152.0
38	4185545.0
39	9026409.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.530671732481196	38.21324276735049	30.13405917052838	20.122026329639926
2	27.11911564936424	43.96421234805691	13.104574403337343	15.812097599241506
3	36.03369252875626	25.534273162141957	18.670696500684862	19.761337808416922
4	21.423629984208574	23.85593975080381	19.986159170212538	34.73427109477508
5	20.01394419813108	35.229443745272455	23.345749977053185	21.41086207954328
6	32.114065435798544	23.544375120656223	21.849005074907115	22.49255436863812
7	22.106660242515535	22.90825781940842	33.63378108097063	21.35130085710542
8	31.549420644361888	24.710752882373622	23.02781096571588	20.712015507548617
9	31.804946232668936	23.930116108491966	23.418203529014395	20.846734129824707
10	20.849284839414885	24.476417804508067	33.88655975125749	20.78773760481957
11	20.605938530483083	35.143437454925895	23.50687538266626	20.743748631924756
12	20.53419324309851	24.114863094850122	34.290945325516006	21.05999833653536
13	20.491836150072842	24.329668255572106	23.583702946945337	31.59479264740972
14	21.036142262788555	35.243766960663464	23.073245181192743	20.64684559535524
15	20.67075909595961	34.892649582367966	23.192099634066494	21.244491687605933
16	17.648953065316448	36.18207874313282	19.59617338048844	26.572794811062288
17	31.861770108229525	23.748695094301123	23.31931926585888	21.07021553161047
18	20.895360321467894	23.945956350036987	23.348075764784568	31.81060756371055
19	21.14682774517368	34.69490019834279	23.279953154998058	20.87831890148547
20	32.02864298517057	24.13791997816248	23.251589072932397	20.581847963734557
21	21.286949277632345	34.60214625223235	23.27677074997466	20.834133720160647
22	31.691083130831878	23.738075911223056	23.717282603213054	20.853558354732016
23	21.14790449875302	24.031890856811586	34.040167024100995	20.78003762033439
24	21.05115938604181	34.36387743462359	23.349257800936115	21.235705378398485
25	20.86738865626226	23.983346019883285	23.449429382815374	31.69983594103908
26	21.18937147548644	30.77433034780672	27.194177337773194	20.84212083893365
27	21.440881969335397	23.59514524831517	34.07899715095788	20.88497563139155
28	28.24700784537015	23.645269323826508	27.19532108935303	20.912401741450314
29	28.59805344009369	23.744450292412772	26.92083985247806	20.736656415015478
30	28.251491925831683	23.660654936082473	27.067871750130383	21.019981387955465
31	25.42499942333864	24.805430628212495	27.83046691959369	21.93910302885518
32	24.239723439910883	25.76455486097771	28.07068825035966	21.925033448751748
33	23.898081493113608	25.2259387927324	28.417565612338276	22.458414101815713
34	23.119670009962604	25.116081214210507	28.75138314979231	23.012865626034582
35	22.859210497476425	25.299770589275173	29.03896249576357	22.80205641748483
36	23.13947270467961	25.256331957098695	29.010052858551045	22.594142479670655
37	23.617776215767254	25.110917582601118	28.84274928017827	22.428556921453364
38	23.49969223989917	25.318783664700657	28.866299077351393	22.315225018048782
39	23.497854580457087	25.156418796078473	28.90294219804934	22.4427844254151
40	23.01378445575562	25.732572886885457	28.604805881429048	22.648836775929876
41	23.379009698726264	25.936988571959635	28.376390555464155	22.307611173849953
42	23.262519318155604	25.889745410469473	28.533936353622337	22.313798917752585
43	22.98549694283338	25.571007208697722	29.22068021538517	22.22281563308373
44	23.421347649466078	25.308652609911896	28.72257879515199	22.54742094547004
45	23.593254947586985	24.947131399254197	28.70486739516464	22.754746257994178
46	23.124498651569528	25.12145062539284	28.560534559817874	23.193516163219762
47	22.962478344092734	25.50394699577616	28.635988665086852	22.897585995044253
48	22.913330525159996	25.149226082168454	28.757006196262218	23.18043719640933
49	22.668280552787103	25.84522045357072	28.49469466761957	22.991804326022606
50	23.486096431370644	25.669609123136873	28.242480694765437	22.601813750727047
51	23.2497801269191	25.342338247445245	28.64102308626672	22.766858539368933
52	23.515877042589576	25.31568739996361	28.384583453810098	22.78385210363672
53	23.969549217353286	25.529889578681157	27.747040052266097	22.753521151699456
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8745.0
1	5140.0
2	1535.0
3	1269.0
4	1003.0
5	1107.5
6	1212.0
7	1364.5
8	1851.0
9	2185.0
10	3163.0
11	4141.0
12	6002.5
13	7864.0
14	10441.5
15	13019.0
16	19085.0
17	25151.0
18	34763.0
19	44375.0
20	111330.0
21	178285.0
22	417824.5
23	657364.0
24	458791.0
25	272905.5
26	285593.0
27	315029.5
28	344466.0
29	375612.0
30	406758.0
31	436044.0
32	465330.0
33	515193.0
34	565056.0
35	633999.0
36	702942.0
37	771123.0
38	839304.0
39	902896.0
40	966488.0
41	1032787.5
42	1155758.5
43	1212430.0
44	1268140.5
45	1323851.0
46	1368649.0
47	1413447.0
48	1448160.5
49	1482874.0
50	1478067.5
51	1473261.0
52	1441603.0
53	1409945.0
54	1373817.0
55	1337689.0
56	1295554.0
57	1253419.0
58	1109504.5
59	840713.0
60	715836.0
61	622220.5
62	528605.0
63	448746.0
64	368887.0
65	301185.5
66	233484.0
67	186406.0
68	139328.0
69	108170.5
70	77013.0
71	57926.0
72	38839.0
73	29207.0
74	19575.0
75	14838.0
76	7511.5
77	4922.0
78	3718.5
79	2515.0
80	2041.5
81	1568.0
82	1155.0
83	742.0
84	516.0
85	290.0
86	207.0
87	124.0
88	107.5
89	91.0
90	68.5
91	46.0
92	35.0
93	15.5
94	7.0
95	5.0
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.0896146E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.54898088566934
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.29607506924347	38.56688840326781
2	12.545063562737932	12.682803564846932
3	4.141741494423791	6.280824349050354
4	1.965203966518419	3.9735623095992456
5	1.1703485387256352	2.9579962956806587
6	0.7569574777548222	2.295805744457579
7	0.5747445629592294	2.0336926349017923
8	0.42546624306226916	1.7205507990441749
9	0.3255109361338045	1.4808821479833614
>10	1.6376324024912439	14.648515736083695
>50	0.10158887952217022	3.5462841196549557
>100	0.05777238547080123	5.09126075091938
>500	0.001161005247996846	0.3785389964680882
>1k	5.812000661797864E-4	0.5914086763222539
>5k	6.662059339277385E-5	0.2332161365368705
>10k+	8.565504864784364E-5	3.5177693351828276
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	460667	2.2045548494923417	No Hit
AAGCAGTGGTATCAAAGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	88131	0.42175719867194644	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	60419	0.2891394422684451	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	37095	0.17752077344788844	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	24782	0.1185960320147074	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.244248580575576E-4	0.0	0.0	0.0	4.785571463752215E-6
2	0.004431439175434551	0.0	0.0	9.57114292750443E-6	3.828457171001772E-5
3	0.0044362247468983035	0.0	0.0	9.57114292750443E-6	3.828457171001772E-5
4	0.004522365033245843	0.0	0.0	2.3927857318761077E-5	5.742685756502658E-5
5	0.0045367217476371	4.785571463752215E-6	0.0	1.1485371513005316E-4	6.22124290287788E-5
6	0.004541507319100853	4.785571463752215E-6	0.0	1.1963928659380538E-4	6.699800049253101E-5
7	0.004541507319100853	4.785571463752215E-6	0.0	2.2492185879635412E-4	6.699800049253101E-5
8	0.004555864033492109	4.785571463752215E-6	0.0	2.2492185879635412E-4	7.656914342003544E-5
9	0.004555864033492109	4.785571463752215E-6	0.0	2.2970743026010633E-4	7.656914342003544E-5
10	0.004570220747883365	4.785571463752215E-6	0.0	2.2970743026010633E-4	7.656914342003544E-5
11	0.004570220747883365	4.785571463752215E-6	0.0	2.2970743026010633E-4	8.135471488378766E-5
12	0.0046755033200859145	4.785571463752215E-6	0.0	2.2970743026010633E-4	9.571142927504431E-5
13	0.004685074463013418	4.785571463752215E-6	4.785571463752215E-6	2.2970743026010633E-4	1.1006814366630096E-4
14	0.0047090023203321795	9.57114292750443E-6	4.785571463752215E-6	2.2970743026010633E-4	1.2921042952130981E-4
15	0.004713787891795932	9.57114292750443E-6	4.785571463752215E-6	2.2970743026010633E-4	1.2921042952130981E-4
16	0.004728144606187189	9.57114292750443E-6	4.785571463752215E-6	2.2970743026010633E-4	1.4356714391256645E-4
17	0.004742501320578445	1.4356714391256645E-5	4.785571463752215E-6	2.3449300172385856E-4	1.5792385830382311E-4
18	0.00475207246350595	1.4356714391256645E-5	4.785571463752215E-6	2.3927857318761076E-4	1.7228057269507975E-4
19	0.004756858034969702	1.4356714391256645E-5	4.785571463752215E-6	2.3927857318761076E-4	2.0099400147759305E-4
20	0.004761643606433454	1.4356714391256645E-5	4.785571463752215E-6	2.44064144651363E-4	2.0099400147759305E-4
21	0.004776000320824711	2.3927857318761077E-5	4.785571463752215E-6	2.44064144651363E-4	2.0099400147759305E-4
22	0.004790357035215968	2.3927857318761077E-5	4.785571463752215E-6	2.488497161151152E-4	2.2013628733260192E-4
23	0.0048238560354622335	2.3927857318761077E-5	4.785571463752215E-6	2.488497161151152E-4	2.2970743026010633E-4
24	0.00483821274985349	2.3927857318761077E-5	4.785571463752215E-6	2.536352875788674E-4	2.2970743026010633E-4
25	0.00488128289302726	2.3927857318761077E-5	4.785571463752215E-6	2.536352875788674E-4	2.3927857318761076E-4
26	0.004909996321809773	2.3927857318761077E-5	4.785571463752215E-6	2.7277757343387626E-4	2.488497161151152E-4
27	0.004976994322302304	3.3499000246265504E-5	4.785571463752215E-6	2.7277757343387626E-4	2.488497161151152E-4
28	0.005020064465476074	4.307014317376994E-5	4.785571463752215E-6	2.7277757343387626E-4	2.5842085904261963E-4
29	0.0050966336088961095	4.307014317376994E-5	4.785571463752215E-6	2.823487163613807E-4	2.823487163613807E-4
30	0.005149274894997384	4.7855714637522155E-5	4.785571463752215E-6	2.871342878251329E-4	2.9191985928888513E-4
31	0.005240200752808676	4.7855714637522155E-5	4.785571463752215E-6	2.9191985928888513E-4	3.014910022163896E-4
32	0.005311984324764959	4.7855714637522155E-5	4.785571463752215E-6	3.014910022163896E-4	3.0627657368014177E-4
33	0.0053933390396487466	4.7855714637522155E-5	4.785571463752215E-6	3.014910022163896E-4	3.206332880713984E-4
34	0.0054507658972137735	4.7855714637522155E-5	4.785571463752215E-6	3.014910022163896E-4	3.206332880713984E-4
35	0.0055512628979525695	4.7855714637522155E-5	4.785571463752215E-6	3.014910022163896E-4	3.3020443099890287E-4
36	0.005646974327227614	5.264128610127437E-5	4.785571463752215E-6	3.014910022163896E-4	3.349900024626551E-4
37	0.005742685756502658	5.264128610127437E-5	4.785571463752215E-6	3.014910022163896E-4	3.3977557392640727E-4
38	0.00585275390016896	5.264128610127437E-5	4.785571463752215E-6	3.349900024626551E-4	3.445611453901595E-4
39	0.005948465329444003	5.742685756502658E-5	4.785571463752215E-6	3.445611453901595E-4	3.4934671685391173E-4
40	0.006115960330675331	5.742685756502658E-5	4.785571463752215E-6	3.445611453901595E-4	3.5891785978141614E-4
41	0.006273884188979154	6.22124290287788E-5	4.785571463752215E-6	3.445611453901595E-4	3.732745741726728E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	243560	0.0	44.38042	1
GTGGTAT	247870	0.0	43.35369	6
AGTGGTA	248790	0.0	43.286884	5
TGGTATC	248255	0.0	43.27605	7
GTATCAA	248455	0.0	43.253506	9
GGTATCA	248515	0.0	43.23928	8
AGAGTAC	244865	0.0	43.15347	19
TCAACGC	210320	0.0	43.074764	12
CAACGCA	210395	0.0	43.063877	13
AGCAGTG	252430	0.0	42.890785	2
AACGCAG	211470	0.0	42.844967	14
ACGCAGA	211235	0.0	42.84368	15
GCAGTGG	252995	0.0	42.74948	3
ATCAACG	212130	0.0	42.720524	11
CGCAGAG	212440	0.0	42.594025	16
GCAGAGT	251135	0.0	42.510265	17
CAGAGTA	250025	0.0	42.497852	18
CAGTGGT	253665	0.0	42.49575	4
GAGTACT	87510	0.0	42.483143	20
GTACTTT	87080	0.0	42.42036	22
>>END_MODULE