FastQCFastQC Report
Thu 26 May 2016
SRR805413_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805413_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24791877
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT531290.21430003061083275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT366350.033.4587141
AGTGGTA481850.026.0775155
CAACGCA404750.026.02273413
TCAACGC404450.026.00717212
GGTATCA483000.025.8062118
GTATCAA486800.025.638569
AACGCAG412750.025.50696614
ACGCAGA413950.025.39896215
TGGTATC493350.025.3648557
GTGGTAT500450.025.0049956
AGAGTAC498300.024.7497519
AGCAGTG511750.024.6457272
GCAGTGG511200.024.6400643
CGCAGAG428700.024.54700516
ATCAACG436200.024.14649211
GCAGAGT515850.024.1400617
CAGTGGT532400.023.698634
CAGAGTA528350.023.45774718
TATCAAC472400.022.31604610
GAGTACT191150.022.23986420
Produced by FastQC (version 0.11.5)