FastQCFastQC Report
Thu 26 May 2016
SRR805412_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805412_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16233582
Sequences flagged as poor quality0
Sequence length53
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGAACTTTTTTTTTTTTTTTTTTTTTTTTTTTT242860.1496034578197221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT755700.042.5562361
AGTGGTA849600.037.5817455
TCAACGC859150.037.50317412
GTGGTAT851150.037.4829376
CAACGCA861850.037.37477513
TGGTATC857850.037.264157
GGTATCA860300.037.2399758
AACGCAG868100.037.0975714
ACGCAGA869100.037.03866215
GTATCAA867950.036.9902659
ATCAACG872200.036.98784611
CGCAGAG878050.036.6718316
CAGTGGT872050.036.6142434
TATCAAC879250.036.5362510
AGCAGTG877700.036.51512
GCAGTGG879450.036.4798473
AGAGTAC743200.036.3219219
ACATGGG552000.035.86730224
GAGTACT274750.035.61565420
GTACATG494250.034.44764722
Produced by FastQC (version 0.11.5)