##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805412_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16233582
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.640848704863785	37.0	35.0	37.0	35.0	37.0
2	35.704039133199316	37.0	35.0	37.0	35.0	37.0
3	37.45617960349109	39.0	37.0	39.0	35.0	39.0
4	37.499888564335336	39.0	37.0	39.0	35.0	39.0
5	37.46121213420427	39.0	37.0	39.0	35.0	39.0
6	37.34762623554062	39.0	37.0	39.0	34.0	39.0
7	37.35251763905218	39.0	37.0	39.0	34.0	39.0
8	38.25060439525916	40.0	38.0	40.0	34.0	40.0
9	38.26063206506118	40.0	38.0	40.0	34.0	40.0
10	38.212385473520264	40.0	38.0	40.0	34.0	40.0
11	38.284109200298495	40.0	38.0	40.0	34.0	40.0
12	38.35513924160423	40.0	38.0	40.0	35.0	40.0
13	38.39428383704841	40.0	38.0	40.0	34.0	40.0
14	38.34918362441512	40.0	38.0	40.0	34.0	40.0
15	38.34224208803701	40.0	38.0	40.0	34.0	40.0
16	38.29754745440655	40.0	38.0	40.0	34.0	40.0
17	38.228671589548135	40.0	38.0	40.0	34.0	40.0
18	38.1713563894894	40.0	38.0	40.0	34.0	40.0
19	38.123108319531696	40.0	38.0	40.0	34.0	40.0
20	38.01079330489106	40.0	38.0	40.0	34.0	40.0
21	38.0130889781442	40.0	38.0	40.0	34.0	40.0
22	37.93393294221818	40.0	38.0	40.0	34.0	40.0
23	32.64619909518429	39.0	34.0	40.0	3.0	40.0
24	23.806216890394246	31.0	3.0	40.0	3.0	40.0
25	29.677771855897237	34.0	17.0	40.0	16.0	40.0
26	34.161087121745524	37.0	30.0	40.0	25.0	40.0
27	36.22841853387626	38.0	35.0	40.0	30.0	40.0
28	36.68748468452619	39.0	36.0	40.0	30.0	40.0
29	37.06511144613678	39.0	37.0	40.0	31.0	40.0
30	37.30497348028303	40.0	37.0	40.0	32.0	40.0
31	37.38506633964087	40.0	37.0	40.0	32.0	40.0
32	37.38450620448401	40.0	37.0	40.0	32.0	40.0
33	37.435052781327	40.0	37.0	40.0	32.0	40.0
34	37.45256425846126	40.0	37.0	40.0	32.0	40.0
35	37.205480096752524	40.0	37.0	40.0	31.0	40.0
36	37.33678475890287	40.0	37.0	40.0	32.0	40.0
37	37.181475105124676	40.0	37.0	40.0	31.0	40.0
38	37.17350625388777	40.0	37.0	40.0	31.0	40.0
39	37.14577651438851	40.0	37.0	40.0	31.0	40.0
40	37.130231885975626	40.0	37.0	40.0	31.0	40.0
41	37.09011578590603	40.0	37.0	40.0	31.0	40.0
42	37.02526546513271	40.0	36.0	40.0	31.0	40.0
43	37.033607247001925	40.0	36.0	40.0	31.0	40.0
44	36.86271976203403	40.0	36.0	40.0	31.0	40.0
45	36.84912664376846	40.0	36.0	40.0	31.0	40.0
46	36.72020266383599	39.0	36.0	40.0	31.0	40.0
47	36.49855478599856	39.0	35.0	40.0	30.0	40.0
48	36.491994927552035	39.0	35.0	40.0	30.0	40.0
49	36.35555714074688	39.0	35.0	40.0	30.0	40.0
50	36.263889325227176	39.0	35.0	40.0	30.0	40.0
51	36.1636794023648	39.0	35.0	40.0	29.0	40.0
52	35.936095065155676	39.0	35.0	40.0	28.0	40.0
53	35.580732644218635	38.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	14.0
13	71.0
14	198.0
15	512.0
16	1278.0
17	2558.0
18	4754.0
19	8368.0
20	13585.0
21	21541.0
22	32834.0
23	46735.0
24	65145.0
25	87677.0
26	114309.0
27	146584.0
28	184377.0
29	229383.0
30	282966.0
31	345633.0
32	427599.0
33	535592.0
34	691152.0
35	944869.0
36	1426040.0
37	2781475.0
38	3927587.0
39	3910744.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.766129865854623	39.58385770928437	16.378954441478164	19.271057983382843
2	28.398587569890616	31.09172701379153	25.843156488814362	14.666528927503492
3	25.796272196733906	24.755577666099818	26.044251971006773	23.403898166159507
4	21.080633959898684	30.103830442350926	23.98065935170685	24.834876246043542
5	21.826895629073114	32.07749220104349	23.29070688157426	22.804905288309136
6	25.358648510230214	25.35734257540942	27.913365023196974	21.370643891163393
7	21.29153627338686	26.478118014865725	29.000260078151573	23.230085633595838
8	24.926722888392717	25.35231595836335	26.778661665675514	22.942299487568423
9	25.668888111077393	25.900580660509796	26.453028050124733	21.977503178288067
10	21.363763093074592	27.201199340971083	30.179260498391546	21.255777067562782
11	21.300961180348242	30.839761674287292	26.659033108034937	21.20024403732953
12	21.384608769648004	25.843840256574303	30.44426670589399	22.327284267883698
13	21.57672286991251	26.68374114844155	25.82344426510428	25.916091716541672
14	21.636112103909046	30.676532141827973	25.86157509784347	21.825780656419514
15	21.768861610456643	29.874417118784997	25.696066339517674	22.660654931240686
16	22.604093169332558	25.903069328753197	25.32762639816647	26.165211103747776
17	26.262657249644594	25.848509589565634	25.500989245626748	22.387843915163025
18	21.992835592292572	26.157689658388396	25.424604378750175	26.424870370568858
19	22.812217291291596	30.06025410781182	25.17295936288122	21.954569238015367
20	26.39104542669634	26.303824996849123	25.431281894531963	21.873847681922573
21	22.751774685340546	29.725312626627936	25.434823934729874	22.088088753301644
22	26.421180488693132	25.81498648911867	25.805315179360903	21.958517842827295
23	19.156191159782235	36.2874810993655	25.622348782911867	18.933978957940397
24	15.23470297559713	51.6880747576228	17.433231926262486	15.643990340517577
25	22.265166122917297	26.071842924130976	25.435427621580992	26.227563331370735
26	22.215676121265165	28.306217321599142	27.60510896486062	21.872997592275077
27	22.644805071363795	25.343833542098103	29.787412291384612	22.223949095153493
28	25.52302381569268	25.664988786824743	26.83575935366575	21.97622804381682
29	25.323258908600703	25.66546311220777	27.03863509606198	21.972642883129552
30	25.10727453743727	25.48310040260985	27.081096457947485	22.3285286020054
31	24.13699576593755	26.225610589209452	27.061211752279934	22.576181892573064
32	23.412491463683125	26.577566183483103	27.477780319833293	22.532162033000482
33	23.290632960735344	26.133967229167293	27.521492175910407	23.05390763418696
34	23.263343851036698	26.373464587174904	27.45281355649049	22.910378005297908
35	23.36065447539551	26.290026440252067	27.70163110027103	22.647687984081394
36	23.127828473099775	26.135039081331524	27.64247594893105	23.09465649663765
37	23.420431793796343	26.387552667057708	27.368266596983954	22.823748942161995
38	23.29416268079343	26.41314775753127	27.62517847262545	22.667511089049846
39	23.241038237894752	26.168482100869667	27.60200428962628	22.988475371609297
40	23.276729682949828	26.486889954416714	27.330228165293402	22.906152197340056
41	23.149419518132227	26.579303323197557	27.586480913454594	22.684796245215626
42	23.222798270893016	26.258955047629044	27.601961169136917	22.916285512341023
43	23.339587036305357	26.48023707891456	27.34928742159309	22.83088846318699
44	23.2094863598188	26.349723677744073	27.638607425027946	22.80218253740918
45	23.250918990029433	26.025525358482188	27.600593633617027	23.122962017871348
46	23.330685735286274	26.31052099284064	27.299655738333044	23.059137533540042
47	23.219508793561396	26.301016004970435	27.5550337565671	22.92444144490107
48	23.14178103144457	26.08887551743047	27.548159118548206	23.22118433257675
49	23.196322290422412	26.574486148528404	27.222297580410782	23.006893980638406
50	23.30887292773708	26.43305094340855	27.460932528631083	22.79714360022329
51	23.21628091692887	26.210647779399523	27.43008905859471	23.142982245076904
52	23.404649694688455	26.44999729572931	27.08649267918812	23.05886033039412
53	23.369900740329523	26.49135600510103	27.114280754549426	23.02446250002002
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9163.0
1	5918.0
2	2673.0
3	2236.5
4	1800.0
5	1790.0
6	1780.0
7	1946.0
8	2548.5
9	2985.0
10	4209.0
11	5433.0
12	7774.0
13	10115.0
14	13142.0
15	16169.0
16	22766.0
17	29363.0
18	40365.5
19	51368.0
20	71247.0
21	91126.0
22	203972.5
23	316819.0
24	265032.0
25	235883.0
26	258521.0
27	289345.5
28	320170.0
29	349980.5
30	379791.0
31	409125.0
32	438459.0
33	479393.5
34	520328.0
35	573772.0
36	627216.0
37	681553.5
38	735891.0
39	784270.5
40	832650.0
41	882931.5
42	972952.5
43	1012692.0
44	1047103.5
45	1081515.0
46	1103655.5
47	1125796.0
48	1133821.0
49	1141846.0
50	1133140.0
51	1124434.0
52	1093012.0
53	1061590.0
54	1009343.5
55	957097.0
56	896033.5
57	834970.0
58	746826.5
59	576691.0
60	494699.0
61	427541.5
62	360384.0
63	302788.5
64	245193.0
65	199318.5
66	153444.0
67	120908.0
68	88372.0
69	67794.5
70	47217.0
71	35074.0
72	22931.0
73	17030.5
74	11130.0
75	8351.0
76	4176.5
77	2781.0
78	2115.5
79	1450.0
80	1089.5
81	729.0
82	547.5
83	366.0
84	245.5
85	125.0
86	103.5
87	82.0
88	67.0
89	52.0
90	39.0
91	26.0
92	19.0
93	6.5
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.6233582E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.62931821466051
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.74266417710471	61.5480192287705
2	9.233379394257451	13.412281004444276
3	2.6699015583576227	5.817393896513214
4	1.1628960615205546	3.3784139241100712
5	0.6551775765375198	2.379255034672681
6	0.3793691864488449	1.6531995218058018
7	0.2621391742191214	1.332729265062223
8	0.16985675555947866	0.9869264280351344
9	0.1212750616556619	0.7927312540042477
>10	0.580187488456565	6.916806150073021
>50	0.01818154856284843	0.8932626867217032
>100	0.004809998190375592	0.5279014900916671
>500	9.953550373325658E-5	0.052242957193390005
>1k	3.5713919054285036E-5	0.030658127440504883
>5k	8.923235488520676E-6	0.05103697014675944
>10k+	1.784647097704135E-5	0.227142060914746
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGAACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24286	0.1496034578197221	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2936146809742916E-4	6.1600699094013874E-6	0.0	1.2320139818802775E-5	6.1600699094013874E-6
2	1.2936146809742916E-4	6.1600699094013874E-6	0.0	1.2320139818802775E-5	1.2320139818802775E-5
3	1.2936146809742916E-4	6.1600699094013874E-6	0.0	1.2320139818802775E-5	1.2320139818802775E-5
4	1.2936146809742916E-4	6.1600699094013874E-6	0.0	1.2320139818802775E-5	1.8480209728204163E-5
5	1.2936146809742916E-4	6.1600699094013874E-6	0.0	1.8480209728204163E-5	1.8480209728204163E-5
6	1.2936146809742916E-4	1.2320139818802775E-5	0.0	2.464027963760555E-5	3.0800349547006936E-5
7	1.3552153800683054E-4	1.2320139818802775E-5	0.0	2.464027963760555E-5	3.0800349547006936E-5
8	1.3552153800683054E-4	1.8480209728204163E-5	0.0	2.464027963760555E-5	4.3120489365809716E-5
9	1.3552153800683054E-4	1.8480209728204163E-5	0.0	3.6960419456408326E-5	4.92805592752111E-5
10	1.3552153800683054E-4	1.8480209728204163E-5	0.0	4.3120489365809716E-5	4.92805592752111E-5
11	1.4168160791623192E-4	2.464027963760555E-5	0.0	4.3120489365809716E-5	5.544062918461249E-5
12	1.4168160791623192E-4	2.464027963760555E-5	0.0	8.624097873161943E-5	6.160069909401387E-5
13	1.478416778256333E-4	2.464027963760555E-5	0.0	1.478416778256333E-4	8.008090882221804E-5
14	1.6016181764443607E-4	2.464027963760555E-5	0.0	2.6488300610425967E-4	8.624097873161943E-5
15	1.6632188755383748E-4	2.464027963760555E-5	0.0	4.620052432051041E-4	8.624097873161943E-5
16	1.7248195746323887E-4	2.464027963760555E-5	0.0	4.620052432051041E-4	9.240104864102082E-5
17	1.7864202737264025E-4	2.464027963760555E-5	0.0	4.620052432051041E-4	9.85611185504222E-5
18	1.9096216719144301E-4	2.464027963760555E-5	0.0	4.620052432051041E-4	9.85611185504222E-5
19	2.032823070102458E-4	2.464027963760555E-5	0.0	4.620052432051041E-4	1.1704132827862636E-4
20	2.2176251673844996E-4	3.0800349547006936E-5	0.0	4.620052432051041E-4	1.1704132827862636E-4
21	2.587229361948583E-4	3.0800349547006936E-5	0.0	4.6816531311450545E-4	1.2320139818802775E-4
22	2.8336321583246384E-4	3.0800349547006936E-5	0.0	4.7432538302390686E-4	1.2936146809742916E-4
23	2.8336321583246384E-4	3.0800349547006936E-5	0.0	4.7432538302390686E-4	1.2936146809742916E-4
24	3.01843425560668E-4	3.0800349547006936E-5	0.0	4.7432538302390686E-4	1.2936146809742916E-4
25	3.2648370519827355E-4	3.6960419456408326E-5	0.0	4.7432538302390686E-4	1.4168160791623192E-4
26	3.757642644734847E-4	3.6960419456408326E-5	0.0	4.8048545293330827E-4	1.478416778256333E-4
27	4.065646140204916E-4	3.6960419456408326E-5	0.0	4.8048545293330827E-4	1.6632188755383748E-4
28	4.558451732957027E-4	3.6960419456408326E-5	0.0	4.8048545293330827E-4	1.7248195746323887E-4
29	5.174458723897166E-4	3.6960419456408326E-5	0.0	4.866455228427096E-4	1.7864202737264025E-4
30	6.221670608495402E-4	3.6960419456408326E-5	0.0	4.989656626615124E-4	1.7864202737264025E-4
31	7.022479696717582E-4	4.92805592752111E-5	0.0	5.051257325709138E-4	1.7864202737264025E-4
32	8.131292280409832E-4	4.92805592752111E-5	0.0	5.051257325709138E-4	1.7864202737264025E-4
33	0.0010102514651418276	4.92805592752111E-5	0.0	5.174458723897166E-4	1.9096216719144301E-4
34	0.001145773003148658	4.92805592752111E-5	0.0	5.174458723897166E-4	1.9096216719144301E-4
35	0.001238174051789679	4.92805592752111E-5	0.0	5.174458723897166E-4	2.1560244682904857E-4
36	0.001367535519887108	5.544062918461249E-5	0.0	5.23605942299118E-4	2.2176251673844996E-4
37	0.0015338574074409456	5.544062918461249E-5	0.0	5.23605942299118E-4	2.2792258664785134E-4
38	0.0017186595047229871	6.160069909401387E-5	0.0	5.23605942299118E-4	2.3408265655725272E-4
39	0.0018849813922768247	7.392083891281665E-5	0.0	5.23605942299118E-4	2.464027963760555E-4
40	0.0020266630001930567	7.392083891281665E-5	0.0	5.23605942299118E-4	2.710430760136611E-4
41	0.0022853859363879147	8.008090882221804E-5	0.0	5.359260821179207E-4	3.01843425560668E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	75570	0.0	42.556236	1
AGTGGTA	84960	0.0	37.581745	5
TCAACGC	85915	0.0	37.503174	12
GTGGTAT	85115	0.0	37.482937	6
CAACGCA	86185	0.0	37.374775	13
TGGTATC	85785	0.0	37.26415	7
GGTATCA	86030	0.0	37.239975	8
AACGCAG	86810	0.0	37.09757	14
ACGCAGA	86910	0.0	37.038662	15
GTATCAA	86795	0.0	36.990265	9
ATCAACG	87220	0.0	36.987846	11
CGCAGAG	87805	0.0	36.67183	16
CAGTGGT	87205	0.0	36.614243	4
TATCAAC	87925	0.0	36.53625	10
AGCAGTG	87770	0.0	36.5151	2
GCAGTGG	87945	0.0	36.479847	3
AGAGTAC	74320	0.0	36.32192	19
ACATGGG	55200	0.0	35.867302	24
GAGTACT	27475	0.0	35.615654	20
GTACATG	49425	0.0	34.447647	22
>>END_MODULE