FastQCFastQC Report
Thu 26 May 2016
SRR805411_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805411_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16264507
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3402682.0920892345522675No Hit
AAGCAGTGGTATCAACGCAGAGAACTTTTTTTTTTTTTTTTTTTTTTTTTTTT525510.3231023233596936No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC277260.1704693539127869No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG219350.1348642169110936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1830150.044.8029941
TCAACGC1878550.043.84631712
CAACGCA1882750.043.8134113
GTGGTAT1866550.043.75056
AGTGGTA1868450.043.719855
ACGCAGA1886300.043.6599415
GTATCAA1873850.043.6565639
GGTATCA1875150.043.6338168
AACGCAG1891000.043.63344614
TGGTATC1871600.043.617397
ATCAACG1890550.043.55185311
CGCAGAG1892700.043.48375316
TATCAAC1890050.043.314710
AGCAGTG1902550.043.227752
GCAGTGG1907100.043.113523
CAGTGGT1907450.042.8567474
AGAGTAC1630400.042.76527419
CAGAGTA1648850.042.42071518
GCAGAGT1670850.042.12095617
ACATGGG1251150.042.090224