##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805411_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16264507
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.716570320883385	37.0	35.0	37.0	35.0	37.0
2	35.864172274019744	37.0	35.0	37.0	35.0	37.0
3	37.74251731085363	39.0	38.0	39.0	35.0	39.0
4	37.630615609806064	39.0	37.0	39.0	35.0	39.0
5	37.59313940471728	39.0	37.0	39.0	35.0	39.0
6	37.68736863650401	39.0	37.0	39.0	35.0	39.0
7	37.505443663309315	39.0	37.0	39.0	35.0	39.0
8	38.60991113963676	40.0	39.0	40.0	36.0	40.0
9	38.57115429321036	40.0	39.0	40.0	36.0	40.0
10	38.1710737374333	40.0	38.0	40.0	34.0	40.0
11	38.31689426553169	40.0	38.0	40.0	35.0	40.0
12	38.37193841780756	40.0	38.0	40.0	35.0	40.0
13	38.54492140462665	40.0	39.0	40.0	35.0	40.0
14	38.597097040814084	40.0	39.0	40.0	36.0	40.0
15	38.465311367876076	40.0	39.0	40.0	35.0	40.0
16	38.45638911772733	40.0	39.0	40.0	35.0	40.0
17	38.44795996583235	40.0	39.0	40.0	35.0	40.0
18	38.40794934638966	40.0	39.0	40.0	35.0	40.0
19	38.38126652102028	40.0	38.0	40.0	35.0	40.0
20	38.26957269593231	40.0	38.0	40.0	34.0	40.0
21	38.14302056619361	40.0	38.0	40.0	34.0	40.0
22	38.19187541313118	40.0	38.0	40.0	34.0	40.0
23	33.096134115838865	40.0	35.0	40.0	3.0	40.0
24	34.97885887349675	40.0	36.0	40.0	17.0	40.0
25	36.558304533915475	40.0	35.0	40.0	30.0	40.0
26	37.253084892151975	40.0	37.0	40.0	32.0	40.0
27	37.59100506397151	40.0	38.0	40.0	33.0	40.0
28	37.66473524220562	40.0	38.0	40.0	33.0	40.0
29	37.724273413267305	40.0	38.0	40.0	33.0	40.0
30	37.646398197006526	40.0	38.0	40.0	33.0	40.0
31	37.53508981243637	40.0	38.0	40.0	33.0	40.0
32	37.62683443156316	40.0	38.0	40.0	33.0	40.0
33	37.66850412373397	40.0	38.0	40.0	33.0	40.0
34	37.6602193352679	40.0	38.0	40.0	33.0	40.0
35	37.641494513175225	40.0	38.0	40.0	33.0	40.0
36	37.58595461885196	40.0	38.0	40.0	33.0	40.0
37	37.19006515229758	40.0	37.0	40.0	32.0	40.0
38	37.17810684332455	40.0	37.0	40.0	32.0	40.0
39	37.197834892874404	40.0	36.0	40.0	32.0	40.0
40	37.1387086617504	40.0	36.0	40.0	32.0	40.0
41	37.01447126556003	40.0	36.0	40.0	32.0	40.0
42	36.97308501265977	40.0	36.0	40.0	31.0	40.0
43	37.03947700351446	40.0	36.0	40.0	32.0	40.0
44	36.838505403207115	39.0	36.0	40.0	31.0	40.0
45	36.65368781236345	39.0	35.0	40.0	31.0	40.0
46	36.60642680408327	39.0	35.0	40.0	31.0	40.0
47	36.5302755257199	39.0	35.0	40.0	31.0	40.0
48	36.46345511733002	39.0	35.0	40.0	31.0	40.0
49	36.494552770643466	39.0	35.0	40.0	31.0	40.0
50	36.46068798765312	39.0	35.0	40.0	30.0	40.0
51	36.37653990987861	39.0	35.0	40.0	30.0	40.0
52	36.2551960535908	39.0	35.0	40.0	30.0	40.0
53	35.829208226231515	38.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	19.0
13	51.0
14	150.0
15	505.0
16	1168.0
17	2407.0
18	4277.0
19	7339.0
20	12037.0
21	18009.0
22	26332.0
23	37156.0
24	51679.0
25	68780.0
26	90372.0
27	115225.0
28	145818.0
29	181670.0
30	224243.0
31	276470.0
32	342234.0
33	432250.0
34	562791.0
35	774179.0
36	1156018.0
37	2022773.0
38	3165156.0
39	6545396.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.307806624572143	37.87739769794437	30.757999612284593	20.056796065198903
2	27.305039125993797	43.7670136574075	13.075760611741874	15.852186604856822
3	36.08350993977253	25.50628186885714	18.564761907631137	19.845446283739186
4	21.517694941506683	23.68090222470315	19.909419941225394	34.89198289256477
5	20.211255096757622	34.97376834108775	23.260815713627224	21.55416084852741
6	32.1830535656568	23.446612922236127	21.74858420239851	22.62174930970856
7	22.252011696388955	22.76041321141797	33.5155317034817	21.47204338871138
8	31.568297766418617	24.597333322184312	22.973404604271128	20.86096430712594
9	31.83144130959518	23.809163105896786	23.294496414800644	21.064899169707388
10	20.956466740737977	24.394031740402582	33.65768787212548	20.99181364673396
11	20.607061744939458	34.95498510960092	23.563167331170874	20.874785814288746
12	20.494958746674584	24.05146371789812	34.19365247283548	21.259925062591815
13	20.628574847058076	24.27948169594074	23.51482279788745	31.57712065911374
14	21.065268071144118	35.108220618061154	23.041313210415783	20.785198100378942
15	20.680141119555607	34.744692845593164	23.256155258809873	21.319010776041353
16	21.053168104019385	23.8405504698052	23.39269182890081	31.713589597274606
17	31.85099923409913	23.61886529975978	23.262223687444077	21.267911778697012
18	21.021079827381183	23.94726750709382	23.25250313458625	31.77914953093875
19	21.175176105860448	34.54883077611882	23.280029330123565	20.995963787897168
20	31.975066935628604	24.048075973037484	23.20209275325714	20.77476433807677
21	21.281893143149066	34.48083609297226	23.329136259709564	20.90813450416911
22	31.60987295833805	23.69067196441921	23.682789770387753	21.016665306854982
23	18.38046489819827	34.17353504781915	29.337864344735443	18.108135709247136
24	20.977666276635375	34.16855487842331	23.530519554020294	21.323259290921023
25	20.905785831688597	23.914324608793862	23.47442809056555	31.705461468951995
26	21.180057901539836	30.732274885429973	27.15008822585277	20.937578987177417
27	21.481782386641047	23.628850231980593	33.90669019356074	20.982677187817618
28	28.381727155947612	23.499826954484387	26.95870216047741	21.15974372909059
29	28.650570226321648	23.694385572215623	26.709220267174405	20.945823934288324
30	28.40680015693067	23.63381195630461	26.81038533784024	21.14900254892448
31	25.320515402034626	24.834450868999593	27.673504029356682	22.171529699609096
32	23.948989047131892	25.94801674591182	27.94552580044388	22.157468406512415
33	23.624915283322146	25.446255456743938	28.312176938409507	22.61665232152441
34	23.074840202657235	25.215495311354964	28.725340399189474	22.984324086798328
35	22.970318128917157	25.510038515154505	28.707294970576115	22.812348385352227
36	22.97832943845147	25.48877749568431	28.743988366816158	22.788904699048057
37	23.710432784713365	25.147974051718876	28.68915731660357	22.45243584696419
38	23.45432296226378	25.35747932599494	28.66923049066289	22.51896722107839
39	23.418803902263992	25.2267529535325	28.704411391012343	22.650031753191165
40	23.05140881306762	25.564291619782882	28.480033240478793	22.904266326670708
41	23.254218526267042	25.83560018142573	28.332915347510994	22.57726594479624
42	23.247645932335974	25.711329584106053	28.42453201932281	22.61649246423516
43	23.19033094578274	25.468573993666084	28.920599929650496	22.42049513090068
44	23.38265156146448	25.330278993393406	28.499332933977033	22.78773651116508
45	23.349850075381934	25.056923028776712	28.577872049856783	23.01535484598457
46	23.11567759170321	25.235022494072524	28.40912423598207	23.2401756782422
47	23.258909722870786	25.335910888660813	28.34930686801635	23.055872520452052
48	23.23183235741483	25.221059574692305	28.465307924796	23.081800143096867
49	22.822653032151543	25.724025941886836	28.42976427136709	23.02355675459453
50	23.381311219577697	25.587913608448137	28.254320896415734	22.77645427555843
51	23.249115389725617	25.39947875456662	28.35511706564484	22.996288790062927
52	23.42237609784299	25.443365728822894	28.102069125120117	23.032189048214
53	23.72231141097606	25.648622488219285	27.548532519307223	23.080533581497427
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6435.0
1	3758.5
2	1082.0
3	869.0
4	656.0
5	679.0
6	702.0
7	809.0
8	1160.0
9	1404.0
10	2018.0
11	2632.0
12	3986.5
13	5341.0
14	7202.0
15	9063.0
16	13410.0
17	17757.0
18	24638.5
19	31520.0
20	45062.0
21	58604.0
22	294673.5
23	530743.0
24	367384.0
25	209419.5
26	214814.0
27	236478.5
28	258143.0
29	281677.5
30	305212.0
31	328700.0
32	352188.0
33	392241.5
34	432295.0
35	485040.5
36	537786.0
37	591887.0
38	645988.0
39	697944.0
40	749900.0
41	800291.0
42	899389.0
43	948096.0
44	997463.0
45	1046830.0
46	1078712.0
47	1110594.0
48	1147616.5
49	1184639.0
50	1181611.0
51	1178583.0
52	1154924.0
53	1131265.0
54	1093051.0
55	1054837.0
56	1003772.5
57	952708.0
58	854268.0
59	661075.5
60	566323.0
61	492957.0
62	419591.0
63	356421.0
64	293251.0
65	238252.5
66	183254.0
67	145278.5
68	107303.0
69	82812.0
70	58321.0
71	43511.0
72	28701.0
73	21094.5
74	13488.0
75	10094.5
76	4904.5
77	3108.0
78	2306.0
79	1504.0
80	1237.0
81	970.0
82	702.0
83	434.0
84	297.5
85	161.0
86	107.5
87	54.0
88	43.5
89	33.0
90	28.5
91	24.0
92	18.5
93	7.5
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.6264507E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.75902078581537
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.75832714744891	42.579698525373864
2	12.466263173410717	13.652807284684842
3	3.7865365123590773	6.220410947595587
4	1.6775013294085075	3.6743332066125345
5	0.9746149311214078	2.6684479635721594
6	0.6307435398075195	2.0723339164103236
7	0.47213706911328185	1.8097634506929705
8	0.3791963190018981	1.6611535291303694
9	0.2839127522529536	1.399210587177978
>10	1.4544070332199184	13.65908783360793
>50	0.07634445566858311	2.8795240499438695
>100	0.038176163597147185	3.540056020726251
>500	0.001240059806695396	0.4579275787071863
>1k	4.6379201890282335E-4	0.5063631251693298
>5k	5.655073529853133E-5	0.21430145861640573
>10k+	7.917102941794386E-5	3.0045805219783293
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	340268	2.0920892345522675	No Hit
AAGCAGTGGTATCAACGCAGAGAACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52551	0.3231023233596936	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	27726	0.1704693539127869	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	21935	0.1348642169110936	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.837371646124903E-5	6.148357278828064E-6	0.0	0.0	6.148357278828064E-6
2	0.004057915804026522	6.148357278828064E-6	0.0	1.8445071836484193E-5	6.148357278828064E-6
3	0.004070212518584178	6.148357278828064E-6	0.0	1.8445071836484193E-5	1.2296714557656128E-5
4	0.004180882949603083	6.148357278828064E-6	0.0	1.8445071836484193E-5	1.2296714557656128E-5
5	0.004180882949603083	6.148357278828064E-6	0.0	1.8445071836484193E-5	1.2296714557656128E-5
6	0.004180882949603083	1.8445071836484193E-5	0.0	1.8445071836484193E-5	1.2296714557656128E-5
7	0.004180882949603083	1.8445071836484193E-5	0.0	1.8445071836484193E-5	1.2296714557656128E-5
8	0.004180882949603083	2.4593429115312257E-5	0.0	1.8445071836484193E-5	1.2296714557656128E-5
9	0.004180882949603083	3.074178639414032E-5	0.0	1.8445071836484193E-5	1.2296714557656128E-5
10	0.004180882949603083	3.074178639414032E-5	0.0	2.4593429115312257E-5	1.2296714557656128E-5
11	0.004187031306881911	3.689014367296839E-5	0.0	3.074178639414032E-5	1.2296714557656128E-5
12	0.004248514879670193	3.689014367296839E-5	0.0	1.352638601342174E-4	2.4593429115312257E-5
13	0.004273108308785504	3.689014367296839E-5	0.0	1.352638601342174E-4	3.074178639414032E-5
14	0.004273108308785504	3.689014367296839E-5	0.0	1.6600564652835773E-4	4.303850095179645E-5
15	0.004273108308785504	3.689014367296839E-5	0.0	1.721540038071858E-4	4.303850095179645E-5
16	0.0042792566660643325	3.689014367296839E-5	0.0	1.8445071836484193E-4	4.303850095179645E-5
17	0.004285405023343161	3.689014367296839E-5	0.0	1.9059907564367E-4	4.303850095179645E-5
18	0.004322295167016129	3.689014367296839E-5	0.0	1.9674743292249805E-4	4.9186858230624513E-5
19	0.004340740238852613	3.689014367296839E-5	0.0	1.9674743292249805E-4	5.533521550945257E-5
20	0.004353036953410269	3.689014367296839E-5	0.0	1.9674743292249805E-4	9.837371646124903E-5
21	0.004365333667967926	3.689014367296839E-5	0.0	2.0289579020132611E-4	1.0452207374007709E-4
22	0.004365333667967926	3.689014367296839E-5	0.0	2.2748921931663838E-4	1.0452207374007709E-4
23	0.004408372168919722	3.689014367296839E-5	0.0	2.2748921931663838E-4	1.1681878829773322E-4
24	0.004432965598035034	3.689014367296839E-5	0.0	2.3363757659546644E-4	1.2296714557656128E-4
25	0.004457559027150346	3.689014367296839E-5	0.0	2.3363757659546644E-4	1.2296714557656128E-4
26	0.004525190957217456	3.689014367296839E-5	0.0	2.3363757659546644E-4	1.414122174130455E-4
27	0.004580526172726908	4.303850095179645E-5	0.0	2.3363757659546644E-4	1.414122174130455E-4
28	0.004629713030957532	4.303850095179645E-5	0.0	2.3363757659546644E-4	1.4756057469187355E-4
29	0.004697344961024641	4.303850095179645E-5	0.0	2.3363757659546644E-4	1.4756057469187355E-4
30	0.00476497689109175	4.303850095179645E-5	0.0	2.397859338742945E-4	1.537089319707016E-4
31	0.004851053892995343	4.303850095179645E-5	0.0	2.4593429115312256E-4	1.537089319707016E-4
32	0.004955575966735419	4.9186858230624513E-5	0.0	2.4593429115312256E-4	1.537089319707016E-4
33	0.005029356254081356	4.9186858230624513E-5	0.0	2.582310057107787E-4	1.9059907564367E-4
34	0.005164620114215574	4.9186858230624513E-5	0.0	2.582310057107787E-4	1.9059907564367E-4
35	0.005306032331628619	4.9186858230624513E-5	0.0	2.582310057107787E-4	1.9059907564367E-4
36	0.00539825769081104	4.9186858230624513E-5	0.0	2.6437936298960677E-4	1.9059907564367E-4
37	0.005472037978156977	4.9186858230624513E-5	0.0	2.705277202684348E-4	1.9059907564367E-4
38	0.005607301838291195	4.9186858230624513E-5	0.0	2.766760775472629E-4	1.9059907564367E-4
39	0.005674933768358303	4.9186858230624513E-5	0.0	2.82824434826091E-4	1.9059907564367E-4
40	0.005785604199377208	4.9186858230624513E-5	0.0	2.82824434826091E-4	1.9674743292249805E-4
41	0.005982351632299706	4.9186858230624513E-5	0.0	2.82824434826091E-4	2.1519250475898223E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	183015	0.0	44.802994	1
TCAACGC	187855	0.0	43.846317	12
CAACGCA	188275	0.0	43.81341	13
GTGGTAT	186655	0.0	43.7505	6
AGTGGTA	186845	0.0	43.71985	5
ACGCAGA	188630	0.0	43.65994	15
GTATCAA	187385	0.0	43.656563	9
GGTATCA	187515	0.0	43.633816	8
AACGCAG	189100	0.0	43.633446	14
TGGTATC	187160	0.0	43.61739	7
ATCAACG	189055	0.0	43.551853	11
CGCAGAG	189270	0.0	43.483753	16
TATCAAC	189005	0.0	43.3147	10
AGCAGTG	190255	0.0	43.22775	2
GCAGTGG	190710	0.0	43.11352	3
CAGTGGT	190745	0.0	42.856747	4
AGAGTAC	163040	0.0	42.765274	19
CAGAGTA	164885	0.0	42.420715	18
GCAGAGT	167085	0.0	42.120956	17
ACATGGG	125115	0.0	42.0902	24
>>END_MODULE