FastQCFastQC Report
Thu 26 May 2016
SRR805410_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805410_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17339783
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT993960.57322516665866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT470000.039.321
TCAACGC520350.035.63274812
CAACGCA521650.035.53493513
ACGCAGA529900.035.00830515
AACGCAG530600.034.92668514
ATCAACG532550.034.8120411
GTATCAA533000.034.668019
AGTGGTA531650.034.663225
GGTATCA534750.034.5501638
TGGTATC537350.034.3217627
GTGGTAT538400.034.211186
CGCAGAG545000.034.02110316
TATCAAC545200.033.90517410
AGAGTAC538650.033.57142619
AGCAGTG553900.033.550822
GCAGTGG565100.032.8692253
CAGTGGT570750.032.3709144
GAGTACT215150.032.1889420
CAGAGTA565250.032.15373618
GCAGAGT577250.031.76214817
Produced by FastQC (version 0.11.5)