##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805410_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17339783
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.803912424970946	37.0	35.0	37.0	33.0	37.0
2	36.04494191190282	37.0	35.0	37.0	35.0	37.0
3	37.90217386226806	39.0	38.0	39.0	35.0	39.0
4	37.89579512038876	39.0	38.0	39.0	35.0	39.0
5	37.8547916084071	39.0	38.0	39.0	35.0	39.0
6	37.71037636399487	39.0	38.0	39.0	35.0	39.0
7	37.72453663347459	39.0	38.0	39.0	35.0	39.0
8	38.6972227391773	40.0	39.0	40.0	36.0	40.0
9	38.65245089860698	40.0	39.0	40.0	36.0	40.0
10	38.58068806282063	40.0	39.0	40.0	36.0	40.0
11	38.63266789440214	40.0	39.0	40.0	36.0	40.0
12	38.673830289571676	40.0	39.0	40.0	36.0	40.0
13	38.70613450006843	40.0	39.0	40.0	36.0	40.0
14	38.685271551552866	40.0	39.0	40.0	36.0	40.0
15	38.65926176815477	40.0	39.0	40.0	36.0	40.0
16	38.62264931458485	40.0	39.0	40.0	36.0	40.0
17	38.56803773149871	40.0	39.0	40.0	36.0	40.0
18	38.5330216070178	40.0	39.0	40.0	35.0	40.0
19	38.50131417446227	40.0	39.0	40.0	35.0	40.0
20	38.36676295199311	40.0	39.0	40.0	35.0	40.0
21	38.34504197659221	40.0	38.0	40.0	35.0	40.0
22	38.29142446592325	40.0	38.0	40.0	35.0	40.0
23	38.182184344521495	40.0	38.0	40.0	34.0	40.0
24	38.07000197176632	40.0	38.0	40.0	34.0	40.0
25	38.12981413896587	40.0	38.0	40.0	34.0	40.0
26	38.1466558722217	40.0	38.0	40.0	34.0	40.0
27	38.15713962510373	40.0	38.0	40.0	34.0	40.0
28	38.09287342292576	40.0	38.0	40.0	34.0	40.0
29	38.0357719009517	40.0	38.0	40.0	34.0	40.0
30	37.99889260436535	40.0	38.0	40.0	34.0	40.0
31	38.05223196853156	40.0	38.0	40.0	34.0	40.0
32	37.99505391734141	40.0	38.0	40.0	34.0	40.0
33	38.03024847542786	40.0	38.0	40.0	34.0	40.0
34	38.02974022223923	40.0	38.0	40.0	34.0	40.0
35	38.013878028346724	40.0	38.0	40.0	34.0	40.0
36	37.969057917276125	40.0	38.0	40.0	34.0	40.0
37	37.892015892009724	40.0	38.0	40.0	33.0	40.0
38	37.82487641281324	40.0	38.0	40.0	33.0	40.0
39	37.83921194400184	40.0	38.0	40.0	33.0	40.0
40	37.772708458923624	40.0	38.0	40.0	33.0	40.0
41	37.71544257503107	40.0	38.0	40.0	33.0	40.0
42	37.65090497383964	40.0	38.0	40.0	33.0	40.0
43	37.61146918620608	40.0	37.0	40.0	33.0	40.0
44	37.53144396328374	40.0	37.0	40.0	33.0	40.0
45	37.44693390914984	40.0	37.0	40.0	33.0	40.0
46	37.375219978243095	40.0	37.0	40.0	33.0	40.0
47	37.239547346123075	40.0	37.0	40.0	32.0	40.0
48	37.09903388064314	40.0	36.0	40.0	32.0	40.0
49	37.032818000086856	40.0	36.0	40.0	32.0	40.0
50	36.96980775364951	39.0	36.0	40.0	31.0	40.0
51	36.87066204923095	39.0	36.0	40.0	31.0	40.0
52	36.76044319585775	39.0	36.0	40.0	31.0	40.0
53	36.284973577812366	39.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	5.0
12	24.0
13	82.0
14	133.0
15	384.0
16	860.0
17	1769.0
18	3236.0
19	5668.0
20	9559.0
21	15030.0
22	22162.0
23	31836.0
24	43629.0
25	58427.0
26	76636.0
27	98455.0
28	123569.0
29	154670.0
30	190906.0
31	233520.0
32	286008.0
33	354588.0
34	452558.0
35	606453.0
36	865913.0
37	1427638.0
38	2727550.0
39	9548511.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	17.98770492110541	32.60813010174349	24.033928221593083	25.37023675555801
2	27.298363537767457	27.065944250859424	25.334082900576092	20.301609310797026
3	23.430927595806704	25.20627853301278	25.83308568509767	25.52970818608284
4	21.02277173826224	28.379190212472672	25.01879060424228	25.579247445022812
5	24.490704410776072	29.051268980701778	24.833569139821414	21.624457468700733
6	25.68791085793865	25.60514165604033	26.222790677368916	22.484156808652102
7	23.642591144306706	26.36474170409168	28.01532752745522	21.977339624146392
8	24.009579589317813	25.67477343862954	26.995539678899092	23.32010729315355
9	24.049989552925776	25.27958971574212	27.78166255021761	22.888758181114493
10	22.36420144358208	26.352180993268487	28.501025647206774	22.782591915942664
11	22.282810574965094	28.452040028413272	26.571214876218463	22.693934520403168
12	22.30319145285728	26.090712899924988	28.39739689937296	23.20869874784477
13	22.48099067906444	26.483785869753962	25.93962104370049	25.09560240748111
14	22.791190639467633	28.52094515831023	25.858553131835617	22.82931107038652
15	22.711085830774238	28.15265335212096	25.79167224872422	23.344588568380583
16	23.09671349405007	26.12960035313014	25.63577641081206	25.13790974200773
17	25.38767642017204	25.84312041275257	25.714156861132576	23.055046305942813
18	22.941596212593897	26.01440860015376	25.67022320867568	25.373771978576663
19	23.302932914443048	28.1728150807885	25.613244410267416	22.911007594501037
20	25.315755104893757	25.987862708547162	25.818598767931526	22.877783418627555
21	23.278515077149468	27.95203953821106	25.73994149753777	23.0295038871017
22	25.128370983650715	25.857705370361323	25.979552339265144	23.03437130672281
23	23.019607569483426	26.012205573737575	28.108771603427797	22.85941525335121
24	23.01642413864118	27.862021110644807	25.832780029600137	23.28877472111387
25	22.947991909702676	26.012251710416447	25.836828523171253	25.20292785670963
26	23.133028827408047	27.078856753859032	27.001877705159288	22.786236713573636
27	23.268930182113582	25.54995642102326	28.150963596257235	23.030149800605926
28	24.669063044214568	25.79196060296718	26.602287929439488	22.93668842337877
29	24.71041880973943	25.74363243184762	26.68619324705505	22.8597555113579
30	24.554401863045232	25.637304688299732	26.675576043829384	23.132717404825655
31	24.116414836333302	26.008370462306246	26.66210413359844	23.21311056776201
32	23.8595200412831	26.125546092474167	26.805127838105008	23.209806028137724
33	23.69534843659808	25.922446664989984	26.865907145435443	23.51629775297649
34	23.595018461303695	26.113867745634415	26.80158108091664	23.489532712145245
35	23.591148747363217	26.02403963186852	26.98617970017272	23.398631920595548
36	23.572330749467856	25.95200874197791	26.9557410262862	23.51991948226803
37	23.71118485162127	26.13619213112413	26.763570224610078	23.38905279264452
38	23.79390214975585	26.09124923881689	26.931807624120786	23.183040987306473
39	23.64339853618699	25.984725414383792	26.92971993940178	23.442156110027444
40	23.632037379014488	26.247808291487846	26.730501760027792	23.38965256946987
41	23.695140821543152	26.176331041743715	26.8820722842956	23.24645585241753
42	23.625082274674373	26.04105253220297	26.909736990364873	23.424128202757785
43	23.63349645148385	26.175627457390902	26.790750495551187	23.40012559557406
44	23.715977299139208	26.02362440175866	26.94766134039855	23.312736958703578
45	23.70032543083152	25.828108690864237	26.9478516541989	23.52371422410534
46	23.640618801284884	26.03906288792657	26.755721222116797	23.564597088671754
47	23.619834227452557	26.03964536349734	26.88078622437201	23.45973418467809
48	23.506280326576174	25.98728023297639	26.883421782152638	23.623017658294803
49	23.619857295791995	26.206902358581996	26.661573561791403	23.511666783834606
50	23.760337715875682	26.11936377750517	26.76975830666393	23.350540199955212
51	23.69569446168963	25.956334055622264	26.8306875582007	23.517283924487405
52	23.790626445555866	26.101924112891144	26.6016362488504	23.505813192702586
53	23.910749056086804	26.094859433938705	26.60282426833139	23.391567241643106
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5599.0
1	3393.5
2	1188.0
3	1014.5
4	841.0
5	890.5
6	940.0
7	1085.5
8	1548.5
9	1866.0
10	2704.0
11	3542.0
12	5256.0
13	6970.0
14	8862.0
15	10754.0
16	15534.0
17	20314.0
18	28107.0
19	35900.0
20	49785.5
21	63671.0
22	142780.0
23	221889.0
24	192731.0
25	187803.0
26	212033.0
27	243515.5
28	274998.0
29	309624.5
30	344251.0
31	375629.0
32	407007.0
33	452819.0
34	498631.0
35	558084.0
36	617537.0
37	677707.5
38	737878.0
39	792875.0
40	847872.0
41	905382.5
42	1013480.0
43	1064067.0
44	1107655.5
45	1151244.0
46	1182156.0
47	1213068.0
48	1230603.5
49	1248139.0
50	1251837.5
51	1255536.0
52	1237465.5
53	1219395.0
54	1175678.5
55	1131962.0
56	1067930.5
57	1003899.0
58	913062.5
59	722583.5
60	622941.0
61	535853.0
62	448765.0
63	377391.0
64	306017.0
65	247197.0
66	188377.0
67	149170.5
68	109964.0
69	84426.0
70	58888.0
71	43545.0
72	28202.0
73	20930.0
74	13658.0
75	10326.0
76	5008.0
77	3022.0
78	2054.5
79	1087.0
80	813.5
81	540.0
82	409.0
83	278.0
84	173.5
85	69.0
86	52.0
87	35.0
88	24.5
89	14.0
90	11.0
91	8.0
92	6.0
93	4.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.7339783E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.4560980264719
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.37841290637802	38.491452204438964
2	14.955593822023856	15.690241911443618
3	4.826299433408883	7.595065085520066
4	2.1695525542399006	4.552250458351629
5	1.1230772269768183	2.945612455479711
6	0.7344757937766887	2.3116640541852447
7	0.4632068647516218	1.7008617292762052
8	0.3544717827500564	1.487536526684417
9	0.2577750976856147	1.2169688213681977
>10	1.5816136922817958	15.484291209722883
>50	0.11141280485117791	3.9919193303199507
>100	0.04353866943760894	3.3786911927312806
>500	3.2310955111019743E-4	0.1097975810416503
>1k	1.8761977273288309E-4	0.20830610812560038
>5k	3.5173268423074475E-5	0.1502487684350237
>10k+	2.3448845615382953E-5	0.6850925628756045
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	99396	0.57322516665866	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0957461232357983E-4	0.0	0.0	0.0	1.730125457740734E-5
2	1.0957461232357983E-4	0.0	0.0	0.0	2.3068339436543123E-5
3	1.0957461232357983E-4	0.0	0.0	0.0	2.3068339436543123E-5
4	1.211087820418514E-4	0.0	0.0	0.0	2.3068339436543123E-5
5	1.211087820418514E-4	0.0	0.0	0.0	3.460250915481468E-5
6	1.211087820418514E-4	0.0	0.0	0.0	7.497210316876514E-5
7	1.211087820418514E-4	0.0	0.0	0.0	7.497210316876514E-5
8	1.211087820418514E-4	0.0	0.0	0.0	9.227335774617249E-5
9	1.211087820418514E-4	0.0	0.0	0.0	9.227335774617249E-5
10	1.211087820418514E-4	0.0	0.0	0.0	9.804044260530827E-5
11	1.211087820418514E-4	0.0	0.0	0.0	1.0380752746444405E-4
12	1.3264295176012295E-4	5.767084859135781E-6	0.0	0.0	1.153416971827156E-4
13	1.4994420633753029E-4	5.767084859135781E-6	0.0	0.0	1.2687586690098717E-4
14	1.787796306332092E-4	5.767084859135781E-6	0.0	0.0	1.5571129119666606E-4
15	1.8454671549234498E-4	5.767084859135781E-6	0.0	1.1534169718271561E-5	1.6724546091493762E-4
16	1.8454671549234498E-4	5.767084859135781E-6	0.0	1.1534169718271561E-5	1.9031380035148076E-4
17	1.8454671549234498E-4	5.767084859135781E-6	0.0	1.1534169718271561E-5	2.306833943654312E-4
18	2.0184797006975232E-4	5.767084859135781E-6	0.0	1.1534169718271561E-5	2.306833943654312E-4
19	2.1338213978802387E-4	5.767084859135781E-6	0.0	1.730125457740734E-5	2.595188186611101E-4
20	2.306833943654312E-4	5.767084859135781E-6	0.0	1.730125457740734E-5	2.710529883793817E-4
21	2.4798464894283857E-4	5.767084859135781E-6	0.0	1.730125457740734E-5	2.710529883793817E-4
22	2.88354242956789E-4	5.767084859135781E-6	0.0	1.730125457740734E-5	2.7682007323851746E-4
23	2.9988841267506057E-4	5.767084859135781E-6	0.0	1.730125457740734E-5	2.88354242956789E-4
24	3.2872383697073946E-4	5.767084859135781E-6	0.0	1.730125457740734E-5	2.941213278159248E-4
25	3.5179217640728263E-4	5.767084859135781E-6	0.0	1.730125457740734E-5	2.9988841267506057E-4
26	4.152301098577762E-4	5.767084859135781E-6	0.0	2.3068339436543123E-5	2.9988841267506057E-4
27	4.613667887308624E-4	5.767084859135781E-6	0.0	2.3068339436543123E-5	3.0565549753419635E-4
28	4.613667887308624E-4	5.767084859135781E-6	0.0	2.3068339436543123E-5	3.229567521116037E-4
29	5.24804722181356E-4	5.767084859135781E-6	0.0	2.3068339436543123E-5	3.3449092182987524E-4
30	5.824755707727139E-4	5.767084859135781E-6	0.0	2.3068339436543123E-5	3.5179217640728263E-4
31	6.516805890823432E-4	5.767084859135781E-6	0.0	2.3068339436543123E-5	3.633263461255542E-4
32	7.266526922511084E-4	5.767084859135781E-6	0.0	2.3068339436543123E-5	3.6909343098468996E-4
33	8.362273045746882E-4	5.767084859135781E-6	0.0	3.460250915481468E-5	3.9792885528036886E-4
34	8.823639834477744E-4	5.767084859135781E-6	0.0	3.460250915481468E-5	4.0369594013950463E-4
35	9.804044260530826E-4	5.767084859135781E-6	0.0	3.460250915481468E-5	4.382984492943193E-4
36	0.0010611436140809836	5.767084859135781E-6	0.0	3.460250915481468E-5	4.5559970387172664E-4
37	0.0011995536507002423	5.767084859135781E-6	0.0	3.460250915481468E-5	4.5559970387172664E-4
38	0.001337963687319501	5.767084859135781E-6	0.0	3.460250915481468E-5	4.671338735899982E-4
39	0.0015167433179527103	5.767084859135781E-6	0.0	3.460250915481468E-5	4.7866804330826975E-4
40	0.0016724546091493764	5.767084859135781E-6	0.0	3.460250915481468E-5	4.7866804330826975E-4
41	0.0019089050883739434	1.1534169718271561E-5	0.0	3.460250915481468E-5	4.959692978856771E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	47000	0.0	39.32	1
TCAACGC	52035	0.0	35.632748	12
CAACGCA	52165	0.0	35.534935	13
ACGCAGA	52990	0.0	35.008305	15
AACGCAG	53060	0.0	34.926685	14
ATCAACG	53255	0.0	34.81204	11
GTATCAA	53300	0.0	34.66801	9
AGTGGTA	53165	0.0	34.66322	5
GGTATCA	53475	0.0	34.550163	8
TGGTATC	53735	0.0	34.321762	7
GTGGTAT	53840	0.0	34.21118	6
CGCAGAG	54500	0.0	34.021103	16
TATCAAC	54520	0.0	33.905174	10
AGAGTAC	53865	0.0	33.571426	19
AGCAGTG	55390	0.0	33.55082	2
GCAGTGG	56510	0.0	32.869225	3
CAGTGGT	57075	0.0	32.370914	4
GAGTACT	21515	0.0	32.18894	20
CAGAGTA	56525	0.0	32.153736	18
GCAGAGT	57725	0.0	31.762148	17
>>END_MODULE