FastQCFastQC Report
Thu 26 May 2016
SRR805409_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805409_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20186135
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2716811.345879238398039No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG223530.11073442241419668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1071250.042.1672821
TCAACGC1075100.042.16710312
CAACGCA1075650.042.14990613
AACGCAG1085050.041.78692214
ATCAACG1087100.041.73406211
ACGCAGA1085950.041.72848515
GGTATCA1090400.041.4073268
GTATCAA1093100.041.32019
GTGGTAT1087900.041.303756
AGTGGTA1091200.041.2111435
CGCAGAG1099450.041.19473616
TGGTATC1093650.041.1446087
TATCAAC1102650.040.9686210
AGAGTAC1082850.040.7802119
CAGAGTA1112550.039.8478718
GCAGTGG1138050.039.7995723
CAGTGGT1130800.039.7991264
AGCAGTG1144350.039.670822
GCAGAGT1130700.039.5948117
GAGTACT466650.039.4562320
Produced by FastQC (version 0.11.5)