##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805409_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20186135
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.16949084111446	35.0	33.0	35.0	32.0	35.0
2	35.138853178183936	35.0	35.0	37.0	35.0	37.0
3	37.665368085569625	39.0	37.0	39.0	35.0	39.0
4	37.678647249708774	39.0	37.0	39.0	35.0	39.0
5	37.63850583581255	39.0	37.0	39.0	35.0	39.0
6	37.565749609818816	39.0	37.0	39.0	35.0	39.0
7	37.558275519310655	39.0	37.0	39.0	35.0	39.0
8	38.561523293092016	40.0	38.0	40.0	36.0	40.0
9	38.52513658508674	40.0	38.0	40.0	36.0	40.0
10	38.34950875935388	40.0	38.0	40.0	35.0	40.0
11	38.450565202303466	40.0	38.0	40.0	35.0	40.0
12	38.52396439437267	40.0	38.0	40.0	36.0	40.0
13	38.577654811086916	40.0	39.0	40.0	36.0	40.0
14	38.55064102167156	40.0	39.0	40.0	35.0	40.0
15	38.528176741114635	40.0	39.0	40.0	35.0	40.0
16	38.47697570634497	40.0	38.0	40.0	35.0	40.0
17	38.41058583032363	40.0	38.0	40.0	35.0	40.0
18	38.38601366730184	40.0	38.0	40.0	35.0	40.0
19	38.3447852696913	40.0	38.0	40.0	35.0	40.0
20	38.193910226004135	40.0	38.0	40.0	34.0	40.0
21	38.14201842006902	40.0	38.0	40.0	34.0	40.0
22	38.122515379987306	40.0	38.0	40.0	34.0	40.0
23	37.86672629505351	40.0	38.0	40.0	34.0	40.0
24	37.75859474832602	40.0	38.0	40.0	33.0	40.0
25	37.87770288864114	40.0	38.0	40.0	34.0	40.0
26	38.01854946476876	40.0	38.0	40.0	34.0	40.0
27	38.05569783418173	40.0	38.0	40.0	34.0	40.0
28	37.99514374594245	40.0	38.0	40.0	34.0	40.0
29	37.95537803546841	40.0	38.0	40.0	34.0	40.0
30	37.93993654555466	40.0	38.0	40.0	34.0	40.0
31	37.98288805657943	40.0	38.0	40.0	34.0	40.0
32	37.914624815498364	40.0	38.0	40.0	33.0	40.0
33	37.94201698343937	40.0	38.0	40.0	34.0	40.0
34	37.94164177540674	40.0	38.0	40.0	33.0	40.0
35	37.91946011457865	40.0	38.0	40.0	33.0	40.0
36	37.904596595633585	40.0	38.0	40.0	33.0	40.0
37	37.75625755004611	40.0	38.0	40.0	33.0	40.0
38	37.70119381446721	40.0	38.0	40.0	33.0	40.0
39	37.70861271858134	40.0	38.0	40.0	33.0	40.0
40	37.621187909423966	40.0	37.0	40.0	33.0	40.0
41	37.55173905257247	40.0	37.0	40.0	33.0	40.0
42	37.51644457941057	40.0	37.0	40.0	33.0	40.0
43	37.47333632713742	40.0	37.0	40.0	33.0	40.0
44	37.3850092154838	40.0	37.0	40.0	33.0	40.0
45	37.27954231951783	40.0	37.0	40.0	32.0	40.0
46	37.2002302075162	40.0	36.0	40.0	32.0	40.0
47	37.09916806758699	40.0	36.0	40.0	32.0	40.0
48	36.94879728090593	40.0	36.0	40.0	31.0	40.0
49	36.877954843757855	39.0	36.0	40.0	31.0	40.0
50	36.80515695550436	39.0	36.0	40.0	31.0	40.0
51	36.706904516392065	39.0	35.0	40.0	31.0	40.0
52	36.56217364047154	39.0	35.0	40.0	31.0	40.0
53	36.07302101169937	38.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	16.0
13	56.0
14	159.0
15	380.0
16	851.0
17	1793.0
18	3609.0
19	6544.0
20	10967.0
21	17609.0
22	26394.0
23	37420.0
24	52282.0
25	70718.0
26	92421.0
27	120283.0
28	151847.0
29	192448.0
30	237393.0
31	294644.0
32	364459.0
33	460078.0
34	594380.0
35	807893.0
36	1170689.0
37	1957497.0
38	3551247.0
39	9962055.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	9.69298976748149	43.55212624903182	31.08472226109654	15.670161722390146
2	31.40445161988662	41.616441185992265	15.134432619221064	11.844674574900049
3	34.687363380855224	25.842455725179686	21.741967939875565	17.72821295408953
4	22.762787428103497	27.24403160882457	21.083793405721305	28.90938755735063
5	19.85585155355396	33.19384815369559	25.363825219637143	21.586475073113302
6	26.854343340119346	25.254250008731237	23.655707246582867	24.23569940456655
7	22.902452599271726	25.071337331292	29.575691433748958	22.450518635687317
8	24.862426611136804	26.40456927490082	26.401879309734134	22.33112480422825
9	26.399803627588938	25.300167664587597	26.362718767114163	21.937309940709305
10	22.048297011785564	26.703120731135506	29.674283858698065	21.57429839838087
11	21.47641438046461	31.04273799813585	25.950975756379318	21.529871865020223
12	21.596615696863218	26.062220430012978	30.308798588734298	22.032365284389506
13	21.526627063576065	26.63431607883332	25.60467370301447	26.234383154576147
14	21.880082541804065	31.199132473849005	25.289630729210916	21.63115425513601
15	21.82652597934176	30.702167601673132	25.256216705179074	22.215089713806037
16	22.082305503257558	26.067169371452238	25.402539911677003	26.447985213613208
17	26.68924982419864	25.811642496198505	25.51169404147946	21.987413638123396
18	22.0573230090852	25.758957819315086	25.31096220252168	26.872756969078033
19	22.173244159914717	30.770174676826446	25.22715220125101	21.82942896200783
20	26.968550443163092	26.152846991264056	25.302619842778224	21.57598272279463
21	22.50044399286936	30.33401886988272	25.177509216103033	21.988027921144884
22	26.587244165363998	26.02604213238443	25.540094723432695	21.846618978818878
23	22.168265495103444	26.008634144178664	30.10740292780168	21.715697432916205
24	22.421097451295157	30.088498863204865	25.17562178198055	22.31478190351942
25	21.938216503555534	26.142166392922668	25.26747195537927	26.652145148142527
26	22.189408720391494	28.50644761862536	27.482933211335403	21.82121044964774
27	22.474723368292146	25.45908367302607	29.938569220903354	22.127623737778432
28	24.938449088941496	25.849965830506928	27.27260072321918	21.938984357332398
29	25.110017346064513	25.79141078765202	27.2166563832056	21.881915483077865
30	25.217492105348548	25.498828775295518	27.180042142787613	22.10363697656832
31	24.112183932189097	26.256532020617122	27.35490474030814	22.27637930688564
32	23.5571346372151	26.51650749388132	27.54773511620724	22.37862275269634
33	23.513981254955443	26.001867123151605	27.618318216934547	22.8658334049584
34	22.982106282356675	26.399154667300106	27.81549811293742	22.8032409374058
35	22.943084448806076	26.33175692127294	28.06215256164689	22.66300606827409
36	23.200994147715747	26.06510359709771	27.928179416218114	22.805722838968432
37	23.13715825243416	26.395989128181295	27.827664879879183	22.63918773950536
38	23.186211724037314	26.37320120964216	27.98861198540483	22.451975080915687
39	23.271924021116476	26.06142285286411	27.908076508950323	22.758576617069092
40	22.947691571467246	26.64208378671796	27.703089273900126	22.70713536791466
41	23.186687297989437	26.595477539410094	27.731014381901243	22.486820780699226
42	23.287850794617196	26.25151372464318	27.67988522815289	22.78075025258674
43	23.04992510948728	26.64214818735731	27.823642316867492	22.484284386287914
44	23.25807292976095	26.395013210800382	27.81871319100957	22.528200668429097
45	23.42975017258133	25.984260979132458	27.7322974407929	22.85369140749331
46	23.06469762537504	26.33365921708143	27.672989405847133	22.928653751696398
47	23.119121119520898	26.361460477699172	27.75284124474546	22.766577158034462
48	23.130698372917845	26.08309614495296	27.70347567773623	23.082729804392965
49	22.938051291146124	26.56383700990804	27.614840582409663	22.88327111653618
50	23.30061202899911	26.448832329715422	27.63222380113875	22.618331840146716
51	23.33914342691159	26.063939431694084	27.64387536296572	22.95304177842861
52	23.323167114457522	26.41403616888523	27.466407016499193	22.796389700158056
53	23.45109155368276	26.40535694425902	27.38403859877089	22.75951290328733
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13903.0
1	8270.5
2	2638.0
3	2144.0
4	1650.0
5	1626.0
6	1602.0
7	1783.0
8	2375.5
9	2787.0
10	3866.0
11	4945.0
12	6933.5
13	8922.0
14	12465.5
15	16009.0
16	22867.0
17	29725.0
18	40890.5
19	52056.0
20	71146.5
21	90237.0
22	272622.5
23	455008.0
24	351228.0
25	270420.0
26	293392.0
27	329879.5
28	366367.0
29	410313.5
30	454260.0
31	493099.5
32	531939.0
33	588862.5
34	645786.0
35	718273.0
36	790760.0
37	859019.5
38	927279.0
39	986025.5
40	1044772.0
41	1104469.5
42	1211763.0
43	1259359.0
44	1306675.0
45	1353991.0
46	1373654.0
47	1393317.0
48	1399872.0
49	1406427.0
50	1398672.5
51	1390918.0
52	1359647.0
53	1328376.0
54	1271143.0
55	1213910.0
56	1144472.5
57	1075035.0
58	962053.0
59	738260.5
60	627450.0
61	537711.0
62	447972.0
63	373643.5
64	299315.0
65	240781.0
66	182247.0
67	143063.0
68	103879.0
69	79534.0
70	55189.0
71	41075.0
72	26961.0
73	20147.5
74	13334.0
75	9852.0
76	4616.5
77	2863.0
78	2129.0
79	1395.0
80	1026.0
81	657.0
82	462.5
83	268.0
84	180.0
85	92.0
86	71.5
87	51.0
88	38.5
89	26.0
90	21.0
91	16.0
92	17.0
93	11.5
94	5.0
95	5.0
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.0186135E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.49881109828284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.71205284996337	35.0016254639213
2	15.833513247424225	15.674801625128213
3	5.427727582316887	8.059981868701291
4	2.4644730324546624	4.879539403611425
5	1.3972535217248179	3.45811940641336
6	0.9009529509705181	2.6757659957118096
7	0.5843938899849694	2.024876193714968
8	0.4444984240401863	1.7601714820039687
9	0.33602416222499143	1.496951687739024
>10	1.7484584844139335	15.369161963245642
>50	0.10544105679895815	3.5833682887150142
>100	0.043904917182344766	3.555120761946679
>500	8.393376166890196E-4	0.27418894514969494
>1k	3.550818025584132E-4	0.29087588745802617
>5k	5.066412768191366E-5	0.17028057641131003
>10k+	6.079695321829322E-5	1.7251704501282847
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	271681	1.345879238398039	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	22353	0.11073442241419668	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4861686003784281E-5	4.953895334594761E-6	0.0	0.0	4.953895334594761E-6
2	3.764960454292018E-4	4.953895334594761E-6	0.0	0.0	4.953895334594761E-6
3	3.764960454292018E-4	4.953895334594761E-6	0.0	0.0	4.953895334594761E-6
4	3.963116267675808E-4	4.953895334594761E-6	0.0	0.0	9.907790669189521E-6
5	4.0126552210217555E-4	4.953895334594761E-6	0.0	0.0	1.9815581338379043E-5
6	4.0126552210217555E-4	4.953895334594761E-6	0.0	0.0	1.9815581338379043E-5
7	4.0126552210217555E-4	4.953895334594761E-6	0.0	4.953895334594761E-6	1.9815581338379043E-5
8	4.0126552210217555E-4	4.953895334594761E-6	0.0	9.907790669189521E-6	2.9723372007568562E-5
9	4.2108110344055464E-4	9.907790669189521E-6	0.0	9.907790669189521E-6	2.9723372007568562E-5
10	4.3098889410974413E-4	9.907790669189521E-6	0.0	9.907790669189521E-6	2.9723372007568562E-5
11	4.3098889410974413E-4	9.907790669189521E-6	0.0	9.907790669189521E-6	5.449284868054236E-5
12	4.359427894443389E-4	9.907790669189521E-6	0.0	1.9815581338379043E-5	6.935453468432664E-5
13	4.607122661173127E-4	9.907790669189521E-6	0.0	2.47694766729738E-5	7.43084300189214E-5
14	4.904356381248813E-4	9.907790669189521E-6	0.0	2.9723372007568562E-5	7.926232535351617E-5
15	5.300668008016393E-4	9.907790669189521E-6	0.0	2.9723372007568562E-5	7.926232535351617E-5
16	5.399745914708288E-4	9.907790669189521E-6	0.0	2.9723372007568562E-5	8.421622068811093E-5
17	5.449284868054236E-4	1.4861686003784281E-5	0.0	2.9723372007568562E-5	8.917011602270569E-5
18	5.746518588129922E-4	1.4861686003784281E-5	0.0	3.467726734216332E-5	9.412401135730044E-5
19	5.845596494821817E-4	1.4861686003784281E-5	0.0	3.467726734216332E-5	9.90779066918952E-5
20	5.99421335485966E-4	1.9815581338379043E-5	0.0	3.467726734216332E-5	1.0898569736108472E-4
21	6.241908121589398E-4	1.9815581338379043E-5	0.0	4.458505801135284E-5	1.23847383364869E-4
22	6.340986028281294E-4	1.9815581338379043E-5	0.0	4.458505801135284E-5	1.2880127869946376E-4
23	6.539141841665083E-4	1.9815581338379043E-5	0.0	4.458505801135284E-5	1.486168600378428E-4
24	7.084070328470507E-4	1.9815581338379043E-5	0.0	4.458505801135284E-5	1.486168600378428E-4
25	7.331765095200245E-4	1.9815581338379043E-5	0.0	4.95389533459476E-5	1.486168600378428E-4
26	7.728076721967826E-4	1.9815581338379043E-5	0.0	4.95389533459476E-5	1.5852465070703234E-4
27	8.718855788886777E-4	1.9815581338379043E-5	0.0	5.449284868054236E-5	1.6347854604162708E-4
28	9.016089508962463E-4	1.9815581338379043E-5	0.0	5.449284868054236E-5	1.8329412738000612E-4
29	0.0010105946482573311	1.9815581338379043E-5	0.0	5.449284868054236E-5	1.882480227146009E-4
30	0.0010452719155994944	1.9815581338379043E-5	0.0	6.440063934973188E-5	1.981558133837904E-4
31	0.0012137043569757163	1.9815581338379043E-5	0.0	6.440063934973188E-5	1.981558133837904E-4
32	0.001302874472998422	3.9631162676758085E-5	0.0	7.926232535351617E-5	2.0806360405297992E-4
33	0.0013821367983519381	3.9631162676758085E-5	0.0	7.926232535351617E-5	2.1797139472216944E-4
34	0.0014366296470324804	4.458505801135284E-5	0.0	8.421622068811093E-5	2.1797139472216944E-4
35	0.0016050620884087022	4.458505801135284E-5	0.0	8.917011602270569E-5	2.1797139472216944E-4
36	0.0016793705184276237	4.458505801135284E-5	0.0	9.90779066918952E-5	2.3283308072595372E-4
37	0.0018279873784654665	4.458505801135284E-5	0.0	1.0403180202648996E-4	2.4274087139514324E-4
38	0.0019270652851573616	4.458505801135284E-5	0.0	1.0403180202648996E-4	2.4274087139514324E-4
39	0.002001373715176283	4.458505801135284E-5	0.0	1.0898569736108472E-4	2.47694766729738E-4
40	0.002105405517202773	4.95389533459476E-5	0.0	1.3375517403405854E-4	2.5760255739892753E-4
41	0.0022391606912368317	4.95389533459476E-5	0.0	1.3870906936865328E-4	2.9227982474109085E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	107125	0.0	42.167282	1
TCAACGC	107510	0.0	42.167103	12
CAACGCA	107565	0.0	42.149906	13
AACGCAG	108505	0.0	41.786922	14
ATCAACG	108710	0.0	41.734062	11
ACGCAGA	108595	0.0	41.728485	15
GGTATCA	109040	0.0	41.407326	8
GTATCAA	109310	0.0	41.3201	9
GTGGTAT	108790	0.0	41.30375	6
AGTGGTA	109120	0.0	41.211143	5
CGCAGAG	109945	0.0	41.194736	16
TGGTATC	109365	0.0	41.144608	7
TATCAAC	110265	0.0	40.96862	10
AGAGTAC	108285	0.0	40.78021	19
CAGAGTA	111255	0.0	39.84787	18
GCAGTGG	113805	0.0	39.799572	3
CAGTGGT	113080	0.0	39.799126	4
AGCAGTG	114435	0.0	39.67082	2
GCAGAGT	113070	0.0	39.59481	17
GAGTACT	46665	0.0	39.45623	20
>>END_MODULE