FastQCFastQC Report
Thu 26 May 2016
SRR805408_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805408_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14985690
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT830850.5544289251946357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT429850.039.8546031
TCAACGC472550.036.0792512
CAACGCA473550.036.04276313
AACGCAG479550.035.547714
ATCAACG481350.035.47335411
AGTGGTA480400.035.4652375
GGTATCA479750.035.4300168
ACGCAGA481450.035.42206215
TGGTATC481200.035.3037227
GTATCAA484750.035.1566779
GTGGTAT484550.035.0790446
CGCAGAG487050.035.01960816
TATCAAC498150.034.23456610
AGAGTAC485950.034.08817719
AGCAGTG503700.033.9786572
GCAGTGG505950.033.8693543
CAGTGGT508500.033.597844
CAGAGTA504800.032.9782118
GAGTACT228950.032.89692320
GCAGAGT518800.032.563917
Produced by FastQC (version 0.11.5)