FastQCFastQC Report
Thu 26 May 2016
SRR805407_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805407_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15561019
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1541070.9903400285032747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT817250.042.5976141
TCAACGC821750.042.10696412
CAACGCA824850.042.0626813
AACGCAG831400.041.688914
ATCAACG830400.041.6768411
ACGCAGA832000.041.65883615
GTATCAA834450.041.432329
GGTATCA833600.041.4238248
GTGGTAT833400.041.4027526
AGTGGTA838050.041.3048135
TGGTATC838650.041.1211477
CGCAGAG844000.041.05260516
TATCAAC843050.040.99294310
AGAGTAC832950.040.5646219
CAGAGTA851550.039.82760618
CAGTGGT871600.039.766124
GCAGTGG877100.039.674843
AGCAGTG878850.039.6279262
GAGTACT341650.039.52319720
GCAGAGT875000.039.3430317
Produced by FastQC (version 0.11.5)