##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805407_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15561019
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.820760452769836	33.0	28.0	35.0	17.0	35.0
2	34.07929724910689	35.0	33.0	36.0	32.0	37.0
3	37.20045531722569	37.0	37.0	39.0	35.0	39.0
4	37.796732399080035	39.0	37.0	39.0	35.0	39.0
5	37.80666895914721	39.0	37.0	39.0	35.0	39.0
6	37.882250706075226	39.0	38.0	39.0	35.0	39.0
7	37.84744662287219	39.0	38.0	39.0	35.0	39.0
8	38.87472176468649	40.0	39.0	40.0	36.0	40.0
9	38.905153255066395	40.0	39.0	40.0	36.0	40.0
10	38.66337962828784	40.0	39.0	40.0	36.0	40.0
11	38.769991155463536	40.0	39.0	40.0	36.0	40.0
12	38.7438009683042	40.0	39.0	40.0	36.0	40.0
13	38.85834308151671	40.0	39.0	40.0	36.0	40.0
14	38.808647557078366	40.0	39.0	40.0	36.0	40.0
15	38.848567500624476	40.0	39.0	40.0	36.0	40.0
16	38.835430443211976	40.0	39.0	40.0	36.0	40.0
17	38.63482230823058	40.0	39.0	40.0	36.0	40.0
18	38.646909755717154	40.0	39.0	40.0	36.0	40.0
19	38.648089562772206	40.0	39.0	40.0	36.0	40.0
20	38.51341901195545	40.0	39.0	40.0	36.0	40.0
21	38.461827660515034	40.0	39.0	40.0	35.0	40.0
22	38.44261677207643	40.0	39.0	40.0	35.0	40.0
23	38.27464595988219	40.0	38.0	40.0	35.0	40.0
24	37.831370426319765	40.0	38.0	40.0	34.0	40.0
25	38.12530850325419	40.0	38.0	40.0	34.0	40.0
26	38.16710203875466	40.0	38.0	40.0	34.0	40.0
27	38.15734245938521	40.0	38.0	40.0	34.0	40.0
28	38.18330933212022	40.0	38.0	40.0	34.0	40.0
29	38.13051465331416	40.0	38.0	40.0	34.0	40.0
30	38.0269862789834	40.0	38.0	40.0	34.0	40.0
31	37.84173600713424	40.0	38.0	40.0	34.0	40.0
32	37.82583936180529	40.0	38.0	40.0	33.0	40.0
33	37.82490548980115	40.0	38.0	40.0	33.0	40.0
34	37.79823416448499	40.0	38.0	40.0	33.0	40.0
35	37.70987330585484	40.0	38.0	40.0	33.0	40.0
36	37.64190005808746	40.0	37.0	40.0	33.0	40.0
37	37.472540133779155	40.0	37.0	40.0	33.0	40.0
38	37.33156453314529	40.0	37.0	40.0	33.0	40.0
39	30.360884592455033	38.0	28.0	40.0	3.0	40.0
40	32.42308868076056	38.0	28.0	40.0	15.0	40.0
41	34.78236920088588	38.0	30.0	40.0	25.0	40.0
42	36.06108507418441	38.0	35.0	40.0	30.0	40.0
43	36.51062183009994	39.0	35.0	40.0	31.0	40.0
44	36.61319351901055	39.0	35.0	40.0	31.0	40.0
45	36.62491813678783	39.0	35.0	40.0	31.0	40.0
46	36.39748547315571	39.0	35.0	40.0	30.0	40.0
47	36.30773383156977	39.0	35.0	40.0	30.0	40.0
48	36.138623826627295	38.0	35.0	40.0	30.0	40.0
49	36.41840492579567	39.0	35.0	40.0	30.0	40.0
50	36.44687362697777	39.0	35.0	40.0	31.0	40.0
51	36.41217750585614	39.0	35.0	40.0	31.0	40.0
52	36.256250570737045	39.0	35.0	40.0	30.0	40.0
53	35.99458576588076	38.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	14.0
13	47.0
14	129.0
15	306.0
16	709.0
17	1382.0
18	2444.0
19	4249.0
20	7332.0
21	11619.0
22	17954.0
23	26296.0
24	37210.0
25	50647.0
26	68032.0
27	88453.0
28	114886.0
29	146616.0
30	186597.0
31	234653.0
32	305169.0
33	403706.0
34	561190.0
35	830718.0
36	1369672.0
37	2704334.0
38	3945320.0
39	4441334.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.070751921837509	43.8300088188312	20.396594850247276	23.702644409084005
2	27.414946283402138	38.983051174219376	18.72349105158216	14.878511490796328
3	30.515334503479497	25.64024888087342	22.588199397481617	21.256217218165467
4	21.42852598534839	26.60809680908429	22.675404483472448	29.287972722094867
5	20.22988982919435	31.841873594524884	26.37010468273318	21.55813189354759
6	26.455189084982162	24.779161313279033	25.087470171458566	23.67817943028024
7	23.682125187302965	25.236374301708647	29.17673964667738	21.904760864311008
8	25.49797028073804	25.920738224148433	26.397198024113973	22.184093470999553
9	26.4888565459627	24.886847063164694	26.549932237728136	22.07436415314447
10	22.06405634489618	26.099633963559842	29.7451407263239	22.091168965220078
11	21.6219709004918	30.617133749402914	25.793297983891673	21.967597366213614
12	21.85371022296162	25.559309451392608	29.98691152552413	22.600068800121637
13	21.98341895219073	26.272437556949196	25.112275744923902	26.631867745936177
14	22.209201081240245	30.852606760521272	25.07364716925029	21.86454498898819
15	22.100795584145228	30.47768915390438	24.885632489748904	22.53588277220149
16	22.46324614088576	25.649721268253707	25.075517226731748	26.81151536412879
17	27.358118385434782	25.432222658426163	25.074469737489558	22.135189218649497
18	22.175263715056193	25.425597128311455	25.03943989786273	27.35969925876962
19	22.44636421303772	30.3560647281518	24.926169680790185	22.271401378020293
20	27.516970450328476	25.69193572734536	24.992727018712593	21.79836680361357
21	22.6391472178011	29.96265861509455	25.148545863223998	22.249648303880356
22	26.937869557257144	25.51646521349277	25.188164091310473	22.357501137939618
23	22.266806563246273	25.688728996475103	30.066623528960413	21.97784091131821
24	22.45562453204382	29.591410433982507	25.33298751193608	22.619977522037598
25	22.19469688970883	25.64602613749138	25.061931998155135	27.097344974644656
26	22.351364007716974	28.223865030946882	27.42722054384742	21.997550417488725
27	22.963772488164174	25.03945917680584	29.678705488374508	22.31806284665548
28	25.307365796545845	25.457972900103776	26.810133706539396	22.424527596810982
29	25.379861048945447	25.462239972844962	27.036892635373043	22.121006342836548
30	25.43701668894563	25.109075440368013	26.979544205941785	22.47436366474458
31	24.692765942898724	25.836489242767456	26.885610768806334	22.58513404552748
32	23.68832015435493	26.41274327857321	27.228685987723555	22.670250579348306
33	23.65321962527004	25.85112196058626	27.30238938722458	23.193269026919126
34	23.32712915523077	26.196812689451765	27.34843392968031	23.12762422563715
35	23.263219458828498	26.167881422161365	27.646692032186326	22.92220708682381
36	23.377421491484586	25.882443816822022	27.56467940820585	23.17545528348754
37	23.412644120542492	26.174410557560527	27.335504185169363	23.077441136727614
38	23.491025876904335	26.177212430625524	27.58384910396935	22.74791258850079
39	20.226683098324088	39.061272272721986	22.29336009422005	18.418684534733877
40	23.348425961050495	26.279757129015778	27.283328938805358	23.08848797112837
41	23.45348334835913	26.29597714648379	27.412645662857937	22.837893842299145
42	23.60783699319434	25.922428344827548	27.375026018540304	23.094708643437812
43	23.457139921235235	26.2393034800613	27.342078304769114	22.961478293934352
44	23.499392938213106	26.114253828749906	27.53011868952798	22.856234543509007
45	23.65253200963253	25.656269682595976	27.413166194321846	23.27803211344964
46	23.631421566929518	25.991543355868917	27.19283357985746	23.1842014973441
47	23.46609177715161	26.055697252217225	27.395346024575893	23.082864946055267
48	23.510870335676604	25.82321247728057	27.286182222385307	23.37973496465752
49	23.364864473207057	26.192082922075986	27.20671441889506	23.236338185821893
50	23.568302307194664	26.123674805615238	27.33368553820286	22.974337348987238
51	23.626119857574878	25.808946059380816	27.24148077963275	23.323453303411558
52	23.62597847865876	26.114240976121167	27.019554439204786	23.240226106015292
53	23.833400627555303	26.102011699876464	27.0630734401134	23.001514232454827
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2865.0
1	1858.0
2	851.0
3	719.5
4	588.0
5	686.0
6	784.0
7	924.5
8	1366.0
9	1667.0
10	2576.0
11	3485.0
12	5308.5
13	7132.0
14	9238.5
15	11345.0
16	16075.0
17	20805.0
18	28469.5
19	36134.0
20	50016.5
21	63899.0
22	190667.0
23	317435.0
24	247886.0
25	193411.0
26	208485.0
27	233345.0
28	258205.0
29	289476.5
30	320748.0
31	352142.0
32	383536.0
33	428184.0
34	472832.0
35	525487.5
36	578143.0
37	631738.0
38	685333.0
39	734665.5
40	783998.0
41	831382.0
42	923763.5
43	968761.0
44	1005680.0
45	1042599.0
46	1064094.0
47	1085589.0
48	1094422.5
49	1103256.0
50	1099616.0
51	1095976.0
52	1074112.5
53	1052249.0
54	1009339.5
55	966430.0
56	907183.0
57	847936.0
58	765572.5
59	599133.0
60	515057.0
61	443023.0
62	370989.0
63	310764.5
64	250540.0
65	204087.5
66	157635.0
67	125731.5
68	93828.0
69	73198.0
70	52568.0
71	40210.0
72	27852.0
73	21216.0
74	14580.0
75	10950.0
76	5662.5
77	4005.0
78	3021.0
79	2037.0
80	1584.5
81	1132.0
82	848.0
83	564.0
84	392.5
85	221.0
86	169.5
87	118.0
88	88.5
89	59.0
90	49.0
91	39.0
92	26.5
93	13.5
94	13.0
95	8.0
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.5561019E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.6731133528501
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.58865159690312	39.68647576888028
2	15.109758645739307	16.521950943454236
3	5.191494193217292	8.515054514892963
4	2.3173129360424163	5.067788513050916
5	1.2644432941627881	3.456552577500664
6	0.791225400438598	2.5955253603500203
7	0.5427393179989967	2.077127377780539
8	0.3792132841385859	1.6586216694892366
9	0.2956329693343852	1.454685735893265
>10	1.4358659044833768	13.599547054143493
>50	0.06749718200154722	2.4972584515389844
>100	0.01560217403285053	1.316747562152522
>500	3.3749951835324076E-4	0.12474320902682193
>1k	1.424918791845253E-4	0.17413262400467155
>5k	7.123723629167547E-5	0.25343862173049325
>10k+	1.1872872715279247E-5	1.0003500161109835
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	154107	0.9903400285032747	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.498420058480746E-5	0.0	0.0	0.0	1.9278943107774626E-5
2	6.426314369258209E-5	0.0	0.0	6.426314369258208E-6	1.9278943107774626E-5
3	6.426314369258209E-5	0.0	0.0	1.2852628738516417E-5	2.5705257477032834E-5
4	7.71157724310985E-5	0.0	0.0	1.2852628738516417E-5	2.5705257477032834E-5
5	7.71157724310985E-5	0.0	0.0	1.2852628738516417E-5	2.5705257477032834E-5
6	8.354208680035672E-5	0.0	0.0	1.2852628738516417E-5	2.5705257477032834E-5
7	8.354208680035672E-5	0.0	0.0	1.2852628738516417E-5	2.5705257477032834E-5
8	8.354208680035672E-5	2.5705257477032834E-5	0.0	1.2852628738516417E-5	3.2131571846291044E-5
9	8.354208680035672E-5	4.498420058480746E-5	0.0	1.2852628738516417E-5	4.498420058480746E-5
10	1.0924734427738955E-4	5.141051495406567E-5	0.0	1.2852628738516417E-5	5.141051495406567E-5
11	1.478052304929388E-4	5.141051495406567E-5	0.0	1.2852628738516417E-5	7.068945806184029E-5
12	1.7993680233922984E-4	5.141051495406567E-5	0.0	1.2852628738516417E-5	8.996840116961492E-5
13	2.699052035088448E-4	5.141051495406567E-5	0.0	1.2852628738516417E-5	8.996840116961492E-5
14	3.727262334169761E-4	5.141051495406567E-5	0.0	1.2852628738516417E-5	1.0282102990813133E-4
15	4.498420058480746E-4	5.141051495406567E-5	0.0	1.2852628738516417E-5	1.0282102990813133E-4
16	5.655156644947223E-4	5.141051495406567E-5	0.0	1.9278943107774626E-5	1.0282102990813133E-4
17	6.876156375106283E-4	5.141051495406567E-5	0.0	1.9278943107774626E-5	1.0282102990813133E-4
18	8.032892961572761E-4	5.141051495406567E-5	0.0	1.9278943107774626E-5	1.0282102990813133E-4
19	8.996840116961492E-4	5.141051495406567E-5	0.0	1.9278943107774626E-5	1.1567365864664775E-4
20	0.0010282102990813134	5.141051495406567E-5	0.0	2.5705257477032834E-5	1.349526017544224E-4
21	0.0012595576163746088	5.141051495406567E-5	0.0	2.5705257477032834E-5	1.349526017544224E-4
22	0.0013752312750212566	5.141051495406567E-5	0.0	2.5705257477032834E-5	1.349526017544224E-4
23	0.0014973312480371626	6.426314369258209E-5	0.0	2.5705257477032834E-5	1.478052304929388E-4
24	0.001696546993484167	6.426314369258209E-5	0.0	2.5705257477032834E-5	1.54231544862197E-4
25	0.0019021890533004297	6.426314369258209E-5	0.0	2.5705257477032834E-5	1.54231544862197E-4
26	0.002184946885547791	7.068945806184029E-5	0.0	3.2131571846291044E-5	1.6065785923145522E-4
27	0.0024098678884718282	7.068945806184029E-5	0.0	3.855788621554925E-5	1.7993680233922984E-4
28	0.0026926257207191893	7.068945806184029E-5	0.0	1.0924734427738955E-4	1.9278943107774627E-4
29	0.002923973038012485	7.068945806184029E-5	0.0	1.0924734427738955E-4	1.9278943107774627E-4
30	0.003148894040936522	7.068945806184029E-5	0.0	1.478052304929388E-4	1.9278943107774627E-4
31	0.003412372930076109	7.71157724310985E-5	0.0	1.478052304929388E-4	1.9278943107774627E-4
32	0.003791525477862343	7.71157724310985E-5	0.0	1.478052304929388E-4	1.9278943107774627E-4
33	0.004202809597494869	8.354208680035672E-5	0.0	1.54231544862197E-4	1.9921574544700445E-4
34	0.004665504232081459	8.354208680035672E-5	0.0	1.54231544862197E-4	1.9921574544700445E-4
35	0.005006098893652144	8.996840116961492E-5	0.0	1.54231544862197E-4	2.249210029240373E-4
36	0.005211740953468407	8.996840116961492E-5	0.0	1.54231544862197E-4	2.313473172932955E-4
37	0.005745125046116839	8.996840116961492E-5	0.0	1.54231544862197E-4	2.313473172932955E-4
38	0.005976472363410134	8.996840116961492E-5	0.0	1.54231544862197E-4	2.313473172932955E-4
39	0.006362051225565627	8.996840116961492E-5	0.0	1.6065785923145522E-4	2.377736316625537E-4
40	0.006715498515874828	8.996840116961492E-5	0.0	1.6065785923145522E-4	2.377736316625537E-4
41	0.007062519491814771	9.639471553887313E-5	0.0	1.6065785923145522E-4	2.5705257477032836E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	81725	0.0	42.597614	1
TCAACGC	82175	0.0	42.106964	12
CAACGCA	82485	0.0	42.06268	13
AACGCAG	83140	0.0	41.6889	14
ATCAACG	83040	0.0	41.67684	11
ACGCAGA	83200	0.0	41.658836	15
GTATCAA	83445	0.0	41.43232	9
GGTATCA	83360	0.0	41.423824	8
GTGGTAT	83340	0.0	41.402752	6
AGTGGTA	83805	0.0	41.304813	5
TGGTATC	83865	0.0	41.121147	7
CGCAGAG	84400	0.0	41.052605	16
TATCAAC	84305	0.0	40.992943	10
AGAGTAC	83295	0.0	40.56462	19
CAGAGTA	85155	0.0	39.827606	18
CAGTGGT	87160	0.0	39.76612	4
GCAGTGG	87710	0.0	39.67484	3
AGCAGTG	87885	0.0	39.627926	2
GAGTACT	34165	0.0	39.523197	20
GCAGAGT	87500	0.0	39.34303	17
>>END_MODULE