FastQCFastQC Report
Thu 26 May 2016
SRR805406_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805406_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13509861
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT257130.19032764289728812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT164600.030.9668881
TCAACGC201300.025.27446612
CAACGCA204850.024.99707213
AACGCAG210050.024.31111514
ACGCAGA210050.024.27755215
ATCAACG211200.024.10085111
AGTGGTA213100.023.8528865
GGTATCA212900.023.8090658
GTATCAA214150.023.6920389
TGGTATC216450.023.320867
CGCAGAG219900.023.26489316
GTGGTAT218050.023.1928446
AGCAGTG221950.022.9441322
TATCAAC226000.022.48097410
GCAGTGG227950.022.422683
AGAGTAC222950.022.13500819
CAGTGGT239500.021.31194
GCAGAGT245650.020.66354617
CAGAGTA242900.020.49114818
GAGTACT102900.020.32555820
Produced by FastQC (version 0.11.5)