##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805406_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13509861
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62155643200178	35.0	32.0	37.0	28.0	37.0
2	34.80462441471455	35.0	35.0	37.0	32.0	37.0
3	37.34872653389994	39.0	37.0	39.0	34.0	39.0
4	37.67578763393642	39.0	37.0	39.0	35.0	39.0
5	37.700980861313084	39.0	38.0	39.0	35.0	39.0
6	37.738119807450275	39.0	38.0	39.0	35.0	39.0
7	37.784871361740876	39.0	38.0	39.0	35.0	39.0
8	38.73775933001827	40.0	39.0	40.0	36.0	40.0
9	38.76139599067674	40.0	39.0	40.0	36.0	40.0
10	38.64621856583128	40.0	39.0	40.0	36.0	40.0
11	38.64204161686045	40.0	39.0	40.0	36.0	40.0
12	38.58254796255861	40.0	39.0	40.0	36.0	40.0
13	38.70291315358463	40.0	39.0	40.0	36.0	40.0
14	38.6092723677912	40.0	39.0	40.0	36.0	40.0
15	38.65857102452794	40.0	39.0	40.0	36.0	40.0
16	38.66907831250077	40.0	39.0	40.0	36.0	40.0
17	38.456501587988214	40.0	39.0	40.0	35.0	40.0
18	38.49013331817404	40.0	39.0	40.0	35.0	40.0
19	38.47061105958085	40.0	39.0	40.0	35.0	40.0
20	38.288741090674435	40.0	39.0	40.0	35.0	40.0
21	38.275166191569255	40.0	38.0	40.0	35.0	40.0
22	38.22671839480806	40.0	38.0	40.0	34.0	40.0
23	38.27277401299688	40.0	38.0	40.0	35.0	40.0
24	37.59954303008743	40.0	38.0	40.0	33.0	40.0
25	37.93142409089183	40.0	38.0	40.0	34.0	40.0
26	37.95660984224782	40.0	38.0	40.0	34.0	40.0
27	37.95980980115191	40.0	38.0	40.0	34.0	40.0
28	37.96323418871593	40.0	38.0	40.0	34.0	40.0
29	37.92007519544428	40.0	38.0	40.0	34.0	40.0
30	37.78493109588618	40.0	38.0	40.0	33.0	40.0
31	37.600543188416225	40.0	38.0	40.0	33.0	40.0
32	37.617299023283806	40.0	38.0	40.0	33.0	40.0
33	37.62337088442287	40.0	38.0	40.0	33.0	40.0
34	37.60895297146285	40.0	38.0	40.0	33.0	40.0
35	37.53526324216067	40.0	38.0	40.0	33.0	40.0
36	37.48323169276131	40.0	37.0	40.0	33.0	40.0
37	37.36547430058681	40.0	37.0	40.0	32.0	40.0
38	37.22428158217172	40.0	37.0	40.0	32.0	40.0
39	29.79674498501502	38.0	23.0	40.0	3.0	40.0
40	32.037625627680406	38.0	23.0	40.0	15.0	40.0
41	34.56831569177507	38.0	30.0	40.0	24.0	40.0
42	35.92946337493776	38.0	35.0	40.0	30.0	40.0
43	36.43069066365672	39.0	35.0	40.0	30.0	40.0
44	36.55282959610021	39.0	36.0	40.0	30.0	40.0
45	36.55576352710069	39.0	36.0	40.0	30.0	40.0
46	36.339382174250346	39.0	35.0	40.0	30.0	40.0
47	36.263433280327604	39.0	35.0	40.0	30.0	40.0
48	36.10184671774195	39.0	35.0	40.0	29.0	40.0
49	36.36956360986986	39.0	35.0	40.0	30.0	40.0
50	36.4189660426558	39.0	35.0	40.0	30.0	40.0
51	36.412541698245455	39.0	35.0	40.0	30.0	40.0
52	36.29821853829584	39.0	35.0	40.0	30.0	40.0
53	36.07683572762148	39.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	17.0
11	38.0
12	66.0
13	152.0
14	360.0
15	677.0
16	1305.0
17	2499.0
18	4055.0
19	6598.0
20	10492.0
21	15825.0
22	22963.0
23	31497.0
24	42648.0
25	56660.0
26	72777.0
27	93333.0
28	117074.0
29	147418.0
30	183638.0
31	228085.0
32	285377.0
33	364915.0
34	481992.0
35	677862.0
36	1066287.0
37	2117765.0
38	3150674.0
39	4326807.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.68255180419695	30.86346336205828	21.55191678137917	26.902068052365603
2	24.20353547678988	24.435269911363264	30.12465487246686	21.236539739379996
3	20.53325345094224	26.65051846203303	27.54661206358822	25.26961602343651
4	20.957454706602828	28.556903731281913	27.598707344213235	22.88693421790202
5	24.279605837543407	28.310498531406058	26.252890388731608	21.157005242318924
6	23.5238837764504	26.432092824641202	28.011183830832902	22.032839568075495
7	23.10486392124982	27.303064035966024	27.731336391988044	21.860735650796112
8	22.21796360451081	26.24446691198377	28.48628864501271	23.051280838492712
9	22.274137387497916	26.076996647115763	29.1396114290147	22.509254536371618
10	22.08451293466306	26.95824183535271	28.557584715342372	22.399660514641862
11	21.944111786198246	27.78977518717624	27.839849721621857	22.42626330500366
12	22.15626052703281	26.822215269276274	28.15164419530297	22.86988000838795
13	22.294515095307048	27.192389322140325	27.06975297525267	23.443342607299957
14	22.56869260164853	27.759930320526614	26.98693198989982	22.684445087925035
15	22.539299257038987	27.479994057673874	26.915036357516932	23.065670327770214
16	22.91435863033676	26.879440136356695	26.66028170089981	23.545919532406735
17	24.053718983489173	26.62989648820221	26.64739481775571	22.668989710552907
18	22.723727505412526	26.824458075475388	26.723924102549983	23.727890316562103
19	23.10774329950545	27.526419405795515	26.61141369256131	22.754423602137727
20	23.98069824700639	26.70403492678422	26.733968617441732	22.581298208767656
21	23.030185136619835	27.3505034581777	26.845983093386376	22.773328311816087
22	23.737823801443998	26.590162548674627	26.832281990169992	22.839731659711376
23	22.791174535400476	26.82448028147736	27.682779267677144	22.70156591544502
24	22.718020562905867	27.147533198157998	27.072225243472158	23.06222099546398
25	22.839028469648948	26.668964247670644	26.878529690275865	23.613477592404543
26	22.91894787074419	26.932238607044145	27.546330787563246	22.602482734648415
27	23.18151904005526	26.305414985394744	27.703105161481677	22.809960813068322
28	23.61603128263126	26.51557999005319	27.02773181752203	22.84065690979352
29	23.582737083675397	26.513566645874448	27.17469113856908	22.729005131881074
30	23.46561522727732	26.442285379546092	27.090937501133432	23.001161892043154
31	23.60748197187225	26.60760906422353	26.92357826627528	22.86133069762894
32	23.294725238105706	26.703931298775018	27.096718463646663	22.90462499947261
33	23.22182293363344	26.58313064805034	27.056673640091482	23.138372778224735
34	23.2883003015353	26.695589244034412	26.942697633972696	23.07341282045759
35	23.22161567761504	26.658579240748665	27.134298420983015	22.985506660653282
36	23.175249545498655	26.589348328602348	27.074793737700187	23.160608388198813
37	23.295243378151707	26.750504686909803	26.88511747086073	23.06913446407776
38	23.31778247015273	26.73195527326299	27.052165821691283	22.898096434893
39	19.92195922667154	40.42828419922307	21.57575862549585	18.073997948609538
40	23.318855760248013	26.729986341088185	26.883577854724045	23.067580043939756
41	23.256945426751617	26.710667119373028	27.091418631176147	22.94096882269921
42	23.22239288768404	26.59518110512018	27.07436442166207	23.108061585533708
43	23.31377798779721	26.71766200999403	26.85015782175701	23.118402180451746
44	23.30071345663734	26.6208808514018	27.077828557969617	23.000577133991236
45	23.29217154787899	26.475187272467128	27.06528956885641	23.167351610797475
46	23.547969886588767	26.60454463595147	26.807041167929114	23.040444309530645
47	23.26784117171894	26.683834866990857	26.98506668573422	23.063257275555983
48	23.217648205262808	26.7094976032692	26.872193577713343	23.20066061375465
49	23.35452600141482	26.721037322293697	26.77266627687731	23.151770399414175
50	23.298159766410624	26.69170319368941	26.980758721351762	23.029378318548208
51	23.30866320534312	26.569836654870098	26.960669691568256	23.160830448218526
52	23.37947814563007	26.73277689533593	26.70730661107468	23.180438347959317
53	23.513979899571137	26.683908886997433	26.84351822716755	22.958592986263884
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4485.0
1	3085.0
2	1685.0
3	1541.0
4	1397.0
5	1460.5
6	1524.0
7	1799.0
8	2494.0
9	2914.0
10	3918.5
11	4923.0
12	6903.5
13	8884.0
14	11729.5
15	14575.0
16	19926.0
17	25277.0
18	34214.0
19	43151.0
20	57985.0
21	72819.0
22	114692.0
23	156565.0
24	160979.0
25	191217.5
26	217042.0
27	246576.5
28	276111.0
29	305763.0
30	335415.0
31	363140.5
32	390866.0
33	427284.0
34	463702.0
35	508683.5
36	553665.0
37	594800.5
38	635936.0
39	673579.0
40	711222.0
41	744663.5
42	808365.0
43	838625.0
44	862800.0
45	886975.0
46	900557.5
47	914140.0
48	918297.5
49	922455.0
50	916448.0
51	910441.0
52	888658.5
53	866876.0
54	825801.5
55	784727.0
56	730840.5
57	676954.0
58	617353.0
59	493886.0
60	430020.0
61	372629.0
62	315238.0
63	266297.0
64	217356.0
65	178040.5
66	138725.0
67	111485.0
68	84245.0
69	65576.0
70	46907.0
71	35990.5
72	25074.0
73	18751.0
74	12428.0
75	9539.5
76	4992.5
77	3334.0
78	2448.5
79	1563.0
80	1220.5
81	878.0
82	628.0
83	378.0
84	277.0
85	176.0
86	131.5
87	87.0
88	77.0
89	67.0
90	52.0
91	37.0
92	24.0
93	8.5
94	6.0
95	4.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.3509861E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.43292838483906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.00396298133694	46.07713086411593
2	15.362921146817222	18.875784691887038
3	4.6230768869680405	8.520274539441369
4	1.8453314867341024	4.534564682832988
5	0.9119198150274523	2.8010952344648583
6	0.5377893775337657	1.9822785789695403
7	0.35197395081013283	1.513595335941343
8	0.2463413781441146	1.210677779339996
9	0.19396529882857086	1.072427068087155
>10	0.8368025385985132	9.095809408719576
>50	0.07552794052123982	3.1426780418306635
>100	0.010216635920612087	0.8148002863209497
>500	7.337492659577345E-5	0.033216634639398945
>1k	7.289256202794767E-5	0.08707106856523666
>5k	1.214763529862084E-5	0.0467087144169234
>10k+	1.214763529862084E-5	0.1918870704269614
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25713	0.19032764289728812	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.628440144572916E-4	0.0	0.0	0.0	7.402000657149618E-6
2	1.628440144572916E-4	0.0	0.0	7.402000657149618E-6	7.402000657149618E-6
3	1.628440144572916E-4	2.2206001971448856E-5	0.0	2.2206001971448856E-5	1.4804001314299236E-5
4	1.628440144572916E-4	2.9608002628598472E-5	0.0	2.9608002628598472E-5	1.4804001314299236E-5
5	1.7024601511444123E-4	2.9608002628598472E-5	0.0	2.9608002628598472E-5	2.2206001971448856E-5
6	1.7024601511444123E-4	3.701000328574809E-5	0.0	3.701000328574809E-5	2.9608002628598472E-5
7	1.7024601511444123E-4	3.701000328574809E-5	0.0	3.701000328574809E-5	2.9608002628598472E-5
8	1.7024601511444123E-4	4.441200394289771E-5	0.0	5.9216005257196944E-5	3.701000328574809E-5
9	1.7024601511444123E-4	4.441200394289771E-5	0.0	5.9216005257196944E-5	3.701000328574809E-5
10	1.7764801577159085E-4	4.441200394289771E-5	0.0	6.661800591434656E-5	5.9216005257196944E-5
11	1.7764801577159085E-4	5.9216005257196944E-5	0.0	7.402000657149618E-5	6.661800591434656E-5
12	1.8505001642874045E-4	5.9216005257196944E-5	0.0	7.402000657149618E-5	7.402000657149618E-5
13	1.9245201708589007E-4	5.9216005257196944E-5	0.0	7.402000657149618E-5	8.14220072286458E-5
14	1.998540177430397E-4	6.661800591434656E-5	0.0	8.882400788579543E-5	8.14220072286458E-5
15	1.998540177430397E-4	7.402000657149618E-5	0.0	8.882400788579543E-5	8.882400788579543E-5
16	2.1465801905733894E-4	7.402000657149618E-5	0.0	1.0362800920009466E-4	1.0362800920009466E-4
17	2.2206001971448856E-4	7.402000657149618E-5	0.0	1.1103000985724428E-4	1.2583401117154352E-4
18	2.3686402102878778E-4	7.402000657149618E-5	0.0	1.1103000985724428E-4	1.4063801248584274E-4
19	2.442660216859374E-4	8.14220072286458E-5	0.0	1.1103000985724428E-4	1.5544201380014198E-4
20	2.5907002300023667E-4	8.882400788579543E-5	0.0	1.1103000985724428E-4	1.7764801577159085E-4
21	2.960800262859847E-4	8.882400788579543E-5	0.0	1.1843201051439389E-4	1.7764801577159085E-4
22	3.1088402760028397E-4	8.882400788579543E-5	0.0	1.2583401117154352E-4	1.7764801577159085E-4
23	3.256880289145832E-4	8.882400788579543E-5	0.0	1.2583401117154352E-4	1.8505001642874045E-4
24	3.256880289145832E-4	8.882400788579543E-5	0.0	1.4063801248584274E-4	1.8505001642874045E-4
25	3.478940308860321E-4	1.0362800920009466E-4	0.0	1.4063801248584274E-4	1.9245201708589007E-4
26	4.145120368003786E-4	1.0362800920009466E-4	0.0	1.4804001314299236E-4	2.1465801905733894E-4
27	4.2931603811467787E-4	1.0362800920009466E-4	0.0	1.4804001314299236E-4	2.1465801905733894E-4
28	4.885320433718748E-4	1.0362800920009466E-4	0.0	3.701000328574809E-4	2.1465801905733894E-4
29	5.255420466576229E-4	1.0362800920009466E-4	0.0	3.701000328574809E-4	2.1465801905733894E-4
30	5.847580519148199E-4	1.1843201051439389E-4	0.0	4.441200394289771E-4	2.2206001971448856E-4
31	6.365720565148672E-4	1.1843201051439389E-4	0.0	4.441200394289771E-4	2.5166802234308705E-4
32	6.957880617720642E-4	1.4063801248584274E-4	0.0	4.441200394289771E-4	2.5907002300023667E-4
33	7.624060676864107E-4	1.4804001314299236E-4	0.0	4.5152204008612674E-4	2.7387402431453586E-4
34	8.14220072286458E-4	1.4804001314299236E-4	0.0	4.5152204008612674E-4	2.7387402431453586E-4
35	8.956420795151038E-4	1.7024601511444123E-4	0.0	4.5152204008612674E-4	2.7387402431453586E-4
36	9.844660874008993E-4	1.7024601511444123E-4	0.0	4.5892404074327636E-4	2.886780256288351E-4
37	0.0010954960972581434	1.7024601511444123E-4	0.0	4.7372804205757555E-4	2.960800262859847E-4
38	0.0012065261071153878	1.7024601511444123E-4	0.0	4.7372804205757555E-4	2.960800262859847E-4
39	0.001339762118944081	1.8505001642874045E-4	0.0	4.7372804205757555E-4	2.960800262859847E-4
40	0.0014433901281441755	1.8505001642874045E-4	0.0	4.8113004271472517E-4	3.0348202694313435E-4
41	0.0016802541491729634	1.9245201708589007E-4	0.0	4.885320433718748E-4	3.182860282574336E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	16460	0.0	30.966888	1
TCAACGC	20130	0.0	25.274466	12
CAACGCA	20485	0.0	24.997072	13
AACGCAG	21005	0.0	24.311115	14
ACGCAGA	21005	0.0	24.277552	15
ATCAACG	21120	0.0	24.100851	11
AGTGGTA	21310	0.0	23.852886	5
GGTATCA	21290	0.0	23.809065	8
GTATCAA	21415	0.0	23.692038	9
TGGTATC	21645	0.0	23.32086	7
CGCAGAG	21990	0.0	23.264893	16
GTGGTAT	21805	0.0	23.192844	6
AGCAGTG	22195	0.0	22.944132	2
TATCAAC	22600	0.0	22.480974	10
GCAGTGG	22795	0.0	22.42268	3
AGAGTAC	22295	0.0	22.135008	19
CAGTGGT	23950	0.0	21.3119	4
GCAGAGT	24565	0.0	20.663546	17
CAGAGTA	24290	0.0	20.491148	18
GAGTACT	10290	0.0	20.325558	20
>>END_MODULE