FastQCFastQC Report
Thu 26 May 2016
SRR805405_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805405_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17109872
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3639732.127268982491511No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG189780.11091842183272907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1393050.044.375111
TCAACGC1433250.043.02553612
CAACGCA1438650.042.98491313
AGTGGTA1435700.042.83595
GGTATCA1438750.042.7271238
ACGCAGA1446050.042.69994415
AACGCAG1448100.042.67357314
GTATCAA1441000.042.6685649
TGGTATC1436950.042.6432727
ATCAACG1446550.042.64294411
GTGGTAT1438350.042.6409766
CGCAGAG1454050.042.4149116
TATCAAC1453650.042.3037210
AGCAGTG1467750.042.1871222
GCAGTGG1469800.042.115493
CAGTGGT1467250.041.956454
AGAGTAC1433750.041.90744419
GAGTACT721150.041.76329420
GCAGAGT1473200.041.4711517
CAGAGTA1454800.041.4545318