##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805405_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17109872
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79976314258809	35.0	32.0	37.0	28.0	37.0
2	35.035469230862745	35.0	35.0	37.0	33.0	37.0
3	37.622399045416586	39.0	37.0	39.0	35.0	39.0
4	37.832602721984124	39.0	37.0	39.0	35.0	39.0
5	37.7951504254386	39.0	37.0	39.0	35.0	39.0
6	37.88816503127551	39.0	38.0	39.0	35.0	39.0
7	37.76892498085316	39.0	37.0	39.0	35.0	39.0
8	38.87248636342808	40.0	39.0	40.0	36.0	40.0
9	38.89842185844523	40.0	39.0	40.0	36.0	40.0
10	38.53681062020803	40.0	38.0	40.0	36.0	40.0
11	38.712277157888735	40.0	39.0	40.0	36.0	40.0
12	38.72911913075679	40.0	39.0	40.0	36.0	40.0
13	38.85855376358163	40.0	39.0	40.0	36.0	40.0
14	38.82963262378585	40.0	39.0	40.0	36.0	40.0
15	38.860158451214595	40.0	39.0	40.0	36.0	40.0
16	38.817739372918744	40.0	39.0	40.0	36.0	40.0
17	38.64158843502745	40.0	39.0	40.0	36.0	40.0
18	38.644936151480266	40.0	39.0	40.0	36.0	40.0
19	38.64235565292365	40.0	39.0	40.0	36.0	40.0
20	38.525371843810404	40.0	39.0	40.0	36.0	40.0
21	38.42526069160541	40.0	39.0	40.0	35.0	40.0
22	38.44540111112462	40.0	39.0	40.0	35.0	40.0
23	38.11659105339888	40.0	38.0	40.0	34.0	40.0
24	37.809197462143494	40.0	38.0	40.0	34.0	40.0
25	38.106075428267374	40.0	38.0	40.0	34.0	40.0
26	38.177425348360295	40.0	38.0	40.0	34.0	40.0
27	38.16420572871615	40.0	38.0	40.0	35.0	40.0
28	38.19332388927281	40.0	38.0	40.0	35.0	40.0
29	38.156658214626034	40.0	38.0	40.0	34.0	40.0
30	38.031689483124126	40.0	38.0	40.0	34.0	40.0
31	37.85543866137631	40.0	38.0	40.0	34.0	40.0
32	37.84842440668171	40.0	38.0	40.0	33.0	40.0
33	37.83626721462323	40.0	38.0	40.0	33.0	40.0
34	37.804191580159106	40.0	38.0	40.0	33.0	40.0
35	37.70916632222614	40.0	38.0	40.0	33.0	40.0
36	37.62413050196986	40.0	37.0	40.0	33.0	40.0
37	37.45562012386767	40.0	37.0	40.0	33.0	40.0
38	37.27942371515111	40.0	37.0	40.0	33.0	40.0
39	30.53675620717677	38.0	30.0	40.0	3.0	40.0
40	32.54248833655798	38.0	29.0	40.0	15.0	40.0
41	34.8474839554615	38.0	30.0	40.0	25.0	40.0
42	36.06514449669758	38.0	35.0	40.0	30.0	40.0
43	36.483162702795205	39.0	35.0	40.0	31.0	40.0
44	36.591278181391424	39.0	35.0	40.0	31.0	40.0
45	36.60250690361681	39.0	35.0	40.0	31.0	40.0
46	36.38350292743277	39.0	35.0	40.0	30.0	40.0
47	36.26949552866322	39.0	35.0	40.0	30.0	40.0
48	36.093949972273315	38.0	35.0	40.0	30.0	40.0
49	36.35415618538818	39.0	35.0	40.0	30.0	40.0
50	36.389514135465184	39.0	35.0	40.0	31.0	40.0
51	36.32235927890051	39.0	35.0	40.0	30.0	40.0
52	36.13605192370814	39.0	35.0	40.0	30.0	40.0
53	35.84602865526989	38.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	10.0
13	22.0
14	90.0
15	262.0
16	601.0
17	1137.0
18	2322.0
19	4115.0
20	7129.0
21	12123.0
22	18273.0
23	26988.0
24	38701.0
25	53619.0
26	71941.0
27	94190.0
28	122336.0
29	157104.0
30	200299.0
31	257818.0
32	333651.0
33	443933.0
34	618133.0
35	916439.0
36	1496620.0
37	2835414.0
38	3980325.0
39	5416275.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.288680125719234	39.33844741795847	26.258028113828086	24.114844342494205
2	26.84899688320287	42.11696615848441	15.307303292508559	15.726733665804163
3	32.332503714814465	26.807950404304602	20.336405789593282	20.52314009128765
4	20.738863505232537	25.234274107953585	21.938872482506007	32.087989904307875
5	19.730457364029373	33.43771946394456	25.38567208451355	21.44615108751252
6	28.742611283123566	24.772756920682983	23.684408626785753	22.800223169407698
7	22.40874157328588	24.424028420551597	31.661581103587448	21.505648902575075
8	28.005013713720363	25.932794821609416	24.991244820534018	21.070946644136203
9	28.44508129575721	24.981583731310206	25.535930368152375	21.03740460478021
10	20.994306678623897	25.777475132484916	32.14403357313252	21.08418461575867
11	20.644596289206604	33.3140598597114	25.02636489624236	21.014978954839638
12	20.613023873001506	25.464229071965004	32.52204925904764	21.40069779598585
13	20.596881145574905	25.846487922294216	24.94473950477245	28.611891427358426
14	21.16910050525217	33.527544799867584	24.426027266597902	20.87732742828234
15	20.92011559174727	33.108120271151066	24.479867529108343	21.491896607993326
16	21.29167886235502	25.30418696294163	24.6115984970548	28.79253567764855
17	29.10124634480024	25.1384639230498	24.594731041821937	21.165558690328016
18	21.121736036365437	25.352778793435743	24.691260109952896	28.83422506024592
19	21.379236501593933	32.90187091989934	24.576829096091426	21.1420634824153
20	29.240651245082372	25.497443814892364	24.502415915209653	20.759489024815615
21	21.520938321455592	32.75171783868401	24.696543609443715	21.030800230416684
22	28.70296750320517	25.062010984068145	25.02974890753128	21.20527260519541
23	21.29152690329887	25.43411195595151	32.271287593501576	21.003073547248043
24	21.2769329893292	32.27349684439486	24.93840398104673	21.511166185229204
25	21.00603674884301	25.291024970847236	24.913634654893972	28.789303625415783
26	21.289165693349428	28.931151559754507	28.811892923570674	20.96778982332539
27	21.739519734571946	24.830799435553928	32.329774296382816	21.099906533491307
28	25.09623099459774	25.008924672259386	28.620202418814124	21.274641914328758
29	25.27196579845834	25.205565535499037	28.46985062190997	21.05261804413265
30	25.199557308201953	24.96976014782577	28.564299019887468	21.26638352408481
31	23.8090559648839	25.68332480804065	28.844289425426446	21.663329801649013
32	23.04757744534851	26.246151929131905	29.06223962400186	21.644031001517718
33	22.900071958457666	25.78001167980684	29.24334559603953	22.07657076569597
34	22.39834991167672	25.90716634233149	29.34634461321511	22.34813913277668
35	22.30750761899329	25.940433686470595	29.512435861589147	22.239622832946967
36	22.319646809748196	25.886961632442368	29.611349517985875	22.18204203982356
37	22.541489497992735	25.962117074867656	29.39203168790509	22.10436173923452
38	22.65438338755544	26.084742188603165	29.37692345097614	21.883950972865257
39	19.468859848863858	38.68304800877528	24.09826911621548	17.74982302614537
40	22.3268005745455	26.20927847969874	29.344474347908623	22.119446597847137
41	22.550344035303127	26.295205481373564	29.24630879763449	21.908141685688822
42	22.57507829398139	26.099710155634128	29.317174319013024	22.008037231371453
43	22.38201431314039	26.17804504908044	29.511804647048205	21.928135990730965
44	22.641139571353893	26.045425705113402	29.33214813062307	21.981286592909637
45	22.66065462091125	25.724342064043494	29.464516157689545	22.150487157355705
46	22.62286941714117	25.78996499798479	29.268296104143854	22.31886948073019
47	22.45730418088458	26.054946524439227	29.277513005357374	22.21023628931882
48	22.386076295602912	25.947318600630094	29.2592253174074	22.407379786359595
49	22.227974586835014	26.191382378547306	29.202772528046967	22.37787050657071
50	22.674196510646016	26.26040101293569	28.987078336997495	22.078324139420797
51	22.62487410776656	26.001474470411	29.182620419369588	22.191031002452853
52	22.673939349166375	26.1065015565283	28.992572241335296	22.22698685297003
53	23.117215605119664	26.299425267471317	28.46642569856747	22.11693342884155
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8576.0
1	5240.0
2	1904.0
3	1564.5
4	1225.0
5	1340.5
6	1456.0
7	1744.5
8	2498.0
9	2963.0
10	4224.0
11	5485.0
12	7804.0
13	10123.0
14	12672.5
15	15222.0
16	21514.5
17	27807.0
18	37125.0
19	46443.0
20	66038.0
21	85633.0
22	360516.5
23	635400.0
24	446115.0
25	269399.0
26	281968.0
27	313570.5
28	345173.0
29	374518.5
30	403864.0
31	429575.0
32	455286.0
33	501305.0
34	547324.0
35	610320.0
36	673316.0
37	733142.5
38	792969.0
39	848332.5
40	903696.0
41	945832.0
42	1020411.5
43	1052855.0
44	1078078.5
45	1103302.0
46	1115472.0
47	1127642.0
48	1134257.0
49	1140872.0
50	1122099.0
51	1103326.0
52	1074927.5
53	1046529.0
54	1006532.5
55	966536.0
56	919829.5
57	873123.0
58	782593.0
59	608266.0
60	524469.0
61	451512.0
62	378555.0
63	316446.5
64	254338.0
65	206337.0
66	158336.0
67	125839.5
68	93343.0
69	72078.5
70	50814.0
71	38227.5
72	25641.0
73	19336.0
74	13031.0
75	9851.0
76	4863.0
77	3055.0
78	2238.5
79	1422.0
80	1052.0
81	682.0
82	498.0
83	314.0
84	231.0
85	148.0
86	100.0
87	52.0
88	45.5
89	39.0
90	31.0
91	23.0
92	18.5
93	9.0
94	4.0
95	5.0
96	6.0
97	4.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.7109872E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.62889814257564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.93515248749398	32.35676458127195
2	17.74220490305675	16.900833403047923
3	6.401049749311357	9.1462483954653
4	2.76871017733499	5.274824600904031
5	1.4047464693026586	3.3453263251279544
6	0.8366578061675203	2.390945365808617
7	0.5540927340569195	1.847357847435679
8	0.38575106706523804	1.469831862531207
9	0.2685790739773704	1.1512912821925916
>10	1.5243069848867958	12.925726594492284
>50	0.09891784155685829	3.2910525649386417
>100	0.07849184093137264	6.471911717759657
>500	7.888251396765726E-4	0.2522228921911528
>1k	4.1255917637655865E-4	0.3757713561809259
>5k	6.249115552571527E-5	0.20775761748772367
>10k+	7.498938663085832E-5	2.592133593164358
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	363973	2.127268982491511	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	18978	0.11091842183272907	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0520242348978415E-4	0.0	0.0	1.7533737248297357E-5	5.844579082765786E-6
2	0.0019228665182299434	0.0	0.0	1.7533737248297357E-5	5.844579082765786E-6
3	0.0019462448345610066	0.0	0.0	2.3378316331063142E-5	5.844579082765786E-6
4	0.002092359311630151	0.0	0.0	2.9222895413828928E-5	5.844579082765786E-6
5	0.002098203890712917	0.0	0.0	2.9222895413828928E-5	5.844579082765786E-6
6	0.0021098930488784484	0.0	0.0	4.09120535793605E-5	5.844579082765786E-6
7	0.0021098930488784484	0.0	0.0	4.09120535793605E-5	5.844579082765786E-6
8	0.0021098930488784484	0.0	0.0	4.09120535793605E-5	1.1689158165531571E-5
9	0.0021098930488784484	0.0	0.0	4.6756632662126285E-5	1.1689158165531571E-5
10	0.0021098930488784484	0.0	0.0	4.6756632662126285E-5	1.7533737248297357E-5
11	0.0021098930488784484	0.0	0.0	6.429036991042364E-5	1.7533737248297357E-5
12	0.0021391159442922776	0.0	0.0	7.013494899318943E-5	1.7533737248297357E-5
13	0.0023086087376924854	0.0	0.0	8.1824107158721E-5	2.3378316331063142E-5
14	0.0023319870540235484	0.0	0.0	8.1824107158721E-5	2.3378316331063142E-5
15	0.0024138111611822694	0.0	0.0	8.1824107158721E-5	2.3378316331063142E-5
16	0.0024488786356788644	0.0	0.0	8.1824107158721E-5	3.5067474496594714E-5
17	0.00245472321476163	0.0	0.0	8.766868624148679E-5	5.2601211744892074E-5
18	0.0025190135846720535	0.0	0.0	9.935784440701835E-5	5.2601211744892074E-5
19	0.0025482364800858827	0.0	0.0	9.935784440701835E-5	5.2601211744892074E-5
20	0.0025540810591686484	0.0	0.0	1.0520242348978415E-4	5.2601211744892074E-5
21	0.0026066822709135407	0.0	0.0	1.0520242348978415E-4	5.2601211744892074E-5
22	0.002624216008161838	0.0	0.0	1.1104700257254993E-4	5.2601211744892074E-5
23	0.002647594324492901	0.0	0.0	1.227361607380815E-4	6.429036991042364E-5
24	0.0026826617989894955	0.0	0.0	1.285807398208473E-4	6.429036991042364E-5
25	0.002729418431651622	0.0	0.0	1.4026989798637885E-4	6.429036991042364E-5
26	0.0027761750643137484	0.0	0.0	1.4026989798637885E-4	7.013494899318943E-5
27	0.0029106003832173612	0.0	0.0	1.4026989798637885E-4	7.013494899318943E-5
28	0.0029339786995484242	0.0	0.0	4.149651148763708E-4	7.013494899318943E-5
29	0.0030450257021209745	0.0	0.0	4.208096939591366E-4	7.013494899318943E-5
30	0.0031210052301969297	0.0	0.0	6.54592857269768E-4	7.597952807595521E-5
31	0.0032612751281833083	0.0	0.0	6.604374363525338E-4	7.597952807595521E-5
32	0.003325565498093732	0.0	0.0	6.721265945180653E-4	8.1824107158721E-5
33	0.00338401128892139	0.0	0.0	6.721265945180653E-4	8.1824107158721E-5
34	0.0034775245542456425	0.0	0.0	6.779711736008312E-4	8.1824107158721E-5
35	0.0035885715568181923	0.0	0.0	6.779711736008312E-4	8.1824107158721E-5
36	0.0036996185593907425	0.0	0.0	6.838157526835969E-4	8.1824107158721E-5
37	0.0038574221946254188	0.0	0.0	6.838157526835969E-4	8.766868624148679E-5
38	0.00393924630178414	0.0	0.0	6.955049108491285E-4	8.766868624148679E-5
39	0.004026914988025626	0.0	0.0	6.955049108491285E-4	8.766868624148679E-5
40	0.004178874044177537	0.0	0.0	7.013494899318943E-4	9.351326532425257E-5
41	0.004360055995743276	0.0	0.0	7.071940690146601E-4	9.351326532425257E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	139305	0.0	44.37511	1
TCAACGC	143325	0.0	43.025536	12
CAACGCA	143865	0.0	42.984913	13
AGTGGTA	143570	0.0	42.8359	5
GGTATCA	143875	0.0	42.727123	8
ACGCAGA	144605	0.0	42.699944	15
AACGCAG	144810	0.0	42.673573	14
GTATCAA	144100	0.0	42.668564	9
TGGTATC	143695	0.0	42.643272	7
ATCAACG	144655	0.0	42.642944	11
GTGGTAT	143835	0.0	42.640976	6
CGCAGAG	145405	0.0	42.41491	16
TATCAAC	145365	0.0	42.30372	10
AGCAGTG	146775	0.0	42.187122	2
GCAGTGG	146980	0.0	42.11549	3
CAGTGGT	146725	0.0	41.95645	4
AGAGTAC	143375	0.0	41.907444	19
GAGTACT	72115	0.0	41.763294	20
GCAGAGT	147320	0.0	41.47115	17
CAGAGTA	145480	0.0	41.45453	18
>>END_MODULE