FastQCFastQC Report
Thu 26 May 2016
SRR805404_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805404_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14922469
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1805621.2100008383331204No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG197280.13220332372612065No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG172230.11541655740749067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT847900.042.9757041
CAACGCA858700.042.34762213
TCAACGC856350.042.34034312
ATCAACG866800.041.97900411
GTATCAA864800.041.9701089
ACGCAGA866450.041.9444315
AGTGGTA865650.041.880035
GGTATCA866700.041.87818
AACGCAG870050.041.84380314
GTGGTAT867550.041.81546
TGGTATC866850.041.7542847
TATCAAC875500.041.44374510
CGCAGAG880400.041.2611316
AGAGTAC850650.040.65984719
CAGTGGT899600.040.3569954
AGCAGTG907050.040.20172
GCAGTGG907600.040.1540343
CAGAGTA870450.039.81325518
GCAGAGT883900.039.4360817
GAGTACT330550.039.12993620
Produced by FastQC (version 0.11.5)