FastQCFastQC Report
Thu 26 May 2016
SRR805403_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805403_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17522446
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT191980.10956232936885638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT159800.025.5294111
CAACGCA197650.020.65241613
TCAACGC197800.020.50606712
AACGCAG206150.019.84647214
ACGCAGA209700.019.40963415
ATCAACG210450.019.39629411
GTATCAA213850.019.1978029
AGTGGTA211950.019.1703225
GGTATCA213950.019.111948
GTGGTAT220150.018.4242576
TGGTATC223400.018.1877787
CGCAGAG226000.018.11371616
TATCAAC227300.018.01011810
AGCAGTG231000.017.8945882
AGAGTAC221600.017.60379219
GCAGTGG249350.016.4928823
CAGTGGT251500.016.2491054
CAGAGTA245350.015.98593918
GCAGAGT259550.015.31053617
GAGTACT89250.014.79775820
Produced by FastQC (version 0.11.5)