##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805403_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17522446
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.656407501555435	37.0	35.0	37.0	35.0	37.0
2	35.7781107158213	37.0	35.0	37.0	35.0	37.0
3	37.585973955919165	39.0	37.0	39.0	35.0	39.0
4	37.62390678789936	39.0	37.0	39.0	35.0	39.0
5	37.582630358798085	39.0	37.0	39.0	35.0	39.0
6	37.48944918991332	39.0	37.0	39.0	35.0	39.0
7	37.473706638901895	39.0	37.0	39.0	35.0	39.0
8	38.47080082312709	40.0	38.0	40.0	35.0	40.0
9	38.4635853350611	40.0	38.0	40.0	35.0	40.0
10	38.47197839844962	40.0	38.0	40.0	35.0	40.0
11	38.44165757451899	40.0	38.0	40.0	35.0	40.0
12	38.39292767687799	40.0	38.0	40.0	35.0	40.0
13	38.44826972216094	40.0	39.0	40.0	35.0	40.0
14	38.44115650292203	40.0	39.0	40.0	35.0	40.0
15	38.44791337921658	40.0	39.0	40.0	35.0	40.0
16	38.413942779449854	40.0	39.0	40.0	35.0	40.0
17	38.35083309716006	40.0	38.0	40.0	34.0	40.0
18	38.24560361036353	40.0	38.0	40.0	34.0	40.0
19	38.252665238631636	40.0	38.0	40.0	34.0	40.0
20	38.197819014537124	40.0	38.0	40.0	34.0	40.0
21	38.16563195572125	40.0	38.0	40.0	34.0	40.0
22	38.15678598752709	40.0	38.0	40.0	34.0	40.0
23	36.91730515248842	40.0	38.0	40.0	32.0	40.0
24	37.352386419110665	40.0	38.0	40.0	32.0	40.0
25	37.70921633886045	40.0	38.0	40.0	32.0	40.0
26	37.73424577824352	40.0	38.0	40.0	33.0	40.0
27	37.80040840188636	40.0	38.0	40.0	33.0	40.0
28	37.84317457733926	40.0	38.0	40.0	34.0	40.0
29	37.658340793288794	40.0	38.0	40.0	33.0	40.0
30	37.67857666675075	40.0	38.0	40.0	33.0	40.0
31	37.56605647407902	40.0	38.0	40.0	33.0	40.0
32	37.67439300426436	40.0	38.0	40.0	33.0	40.0
33	37.461151941914956	40.0	38.0	40.0	32.0	40.0
34	37.645019993213275	40.0	38.0	40.0	33.0	40.0
35	37.69488757448589	40.0	38.0	40.0	33.0	40.0
36	37.738716615248805	40.0	38.0	40.0	33.0	40.0
37	37.541788229793944	40.0	38.0	40.0	33.0	40.0
38	37.56006199134527	40.0	38.0	40.0	33.0	40.0
39	37.61835893230888	40.0	38.0	40.0	33.0	40.0
40	37.598517238974516	40.0	38.0	40.0	33.0	40.0
41	37.503409455506386	40.0	37.0	40.0	33.0	40.0
42	37.42243879650135	40.0	37.0	40.0	33.0	40.0
43	36.6070743776297	40.0	36.0	40.0	30.0	40.0
44	36.73719667904812	40.0	36.0	40.0	30.0	40.0
45	36.94268768184533	40.0	36.0	40.0	31.0	40.0
46	37.001767219028665	40.0	36.0	40.0	31.0	40.0
47	36.989842057438786	40.0	36.0	40.0	31.0	40.0
48	36.9771842355799	40.0	36.0	40.0	31.0	40.0
49	36.81488977052633	40.0	36.0	40.0	31.0	40.0
50	36.72500757029013	39.0	36.0	40.0	31.0	40.0
51	36.55310029204827	39.0	35.0	40.0	30.0	40.0
52	36.519486777131455	39.0	35.0	40.0	30.0	40.0
53	36.09825369129401	38.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	26.0
11	77.0
12	209.0
13	422.0
14	719.0
15	1149.0
16	1946.0
17	3351.0
18	5602.0
19	9023.0
20	13754.0
21	20620.0
22	29688.0
23	41322.0
24	55963.0
25	73652.0
26	95545.0
27	120836.0
28	150986.0
29	186722.0
30	230820.0
31	282451.0
32	344850.0
33	424839.0
34	539216.0
35	715692.0
36	1024725.0
37	1655156.0
38	2955771.0
39	8537308.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.602064460635233	28.792858029067403	22.284411662618336	25.32066584767903
2	26.027359422308965	23.319227235740946	28.384809974589164	22.268603367360924
3	21.26110133254227	25.50300911185573	26.778567330154708	26.457322225447292
4	22.28320749283519	28.266864112464663	26.178633964687346	23.271294430012794
5	25.706542340036314	27.221096871977807	24.884419675198313	22.187941112787566
6	24.525000676275447	25.608759188072263	26.944229133307076	22.922011002345222
7	24.016327400866295	26.521902250404995	26.670334723816524	22.791435624912186
8	23.242057644235288	25.62232464577149	27.182209607037738	23.95340810295549
9	23.066945105723253	25.58125161293121	27.77717791226179	23.574625369083748
10	23.049658706324447	26.29643144570113	27.298894229721128	23.355015618253297
11	22.972323612810676	26.691587464444176	26.89978328368083	23.43630563906432
12	23.11048925475359	26.0666632957522	27.05401403434201	23.768833415152198
13	23.292604240298413	26.414337359065055	26.274870528920445	24.018187871716083
14	23.368563955055134	26.714329723144814	26.324315680584775	23.592790641215274
15	23.37827150387566	26.40435016892048	26.27736447297369	23.94001385423017
16	23.735504734898313	26.154350825221545	26.045941302943664	24.064203136936477
17	24.227507963214727	26.018456555665804	26.1292287617836	23.624806719335872
18	23.7416054813352	26.034116469812492	26.068837649720823	24.155440399131493
19	23.949162120402598	26.52544627616487	25.96863474425888	23.556756859173657
20	24.196136772229174	26.011237243932722	26.212430616136583	23.580195367701517
21	23.815122614730843	26.29812641454281	26.171015165348493	23.715735805377857
22	24.158470797969645	25.940516523777557	26.200668559629175	23.70034411862362
23	22.948274458942546	28.52241062691818	25.826382914805386	22.702931999333885
24	23.621993185198004	26.24633569993596	26.2114775528485	23.92019356201754
25	23.755336441042534	26.019717795106917	26.133645953310396	24.09129981054015
26	24.00280759889344	26.087505134842477	26.52092635925373	23.388760907010354
27	23.934021540143423	25.66184538391501	26.673211034578166	23.730922041363403
28	24.34833584306666	25.855248747806097	26.159253108841085	23.637162300286157
29	24.245872979149144	25.835188763030004	26.264409660614735	23.65452859720612
30	24.14534477663678	25.7470617971943	26.28575941966093	23.821834006507995
31	24.15662174105145	25.95383658194752	26.16542233886753	23.7241193381335
32	23.995753789168475	25.98857488275324	26.318785630727582	23.6968856973507
33	23.94612030763285	25.78799215588965	26.30798805143985	23.957899485037647
34	24.028306322073984	26.02044257976312	26.114350701951082	23.836900396211806
35	23.967880968216424	25.958288015269098	26.31533291642046	23.758498100094016
36	23.88490168552952	25.855385714985225	26.23400294684886	24.025709652636394
37	24.241438666724953	25.971020255961978	26.0453249506376	23.742216126675466
38	24.041774761354663	26.015272068751134	26.253880308719456	23.689072861174747
39	23.959120775718183	25.868100834780716	26.224266863199354	23.94851152630175
40	24.071034374995364	26.020602374805435	26.04561600589324	23.86274724430596
41	23.966482761596183	26.014336126360444	26.273677773068897	23.74550333897448
42	23.967618447789764	25.83062889735828	26.251015411889412	23.950737242962543
43	24.08929095857964	25.85182456832796	26.13609424163727	23.922790231455128
44	24.218120004478827	25.85273197588967	26.223884496490957	23.705263523140548
45	24.00253937150099	25.76024488818513	26.26880973124414	23.96840600906974
46	24.088001184309544	25.925724068432	26.082751232333663	23.9035235149248
47	24.01019241263463	25.93230419999582	26.216699426552665	23.840803960816885
48	23.96275040596501	25.774021503618844	26.227999218830522	24.035228871585623
49	24.133976500769354	25.970295471305775	25.9938024634232	23.90192556450167
50	24.120268368925206	25.95300907190697	26.164532052203214	23.762190506964608
51	24.070629180423783	25.80875409745877	26.19043026298954	23.93018645912791
52	24.160331268819434	25.94443150231423	25.96112437727016	23.934112851596176
53	24.118835920510186	25.953151746051894	26.100248789466953	23.827763543970974
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4370.0
1	2922.0
2	1474.0
3	1321.0
4	1168.0
5	1170.0
6	1172.0
7	1301.5
8	1771.5
9	2112.0
10	2882.5
11	3653.0
12	5277.0
13	6901.0
14	8906.5
15	10912.0
16	15220.0
17	19528.0
18	27041.5
19	34555.0
20	46461.5
21	58368.0
22	90225.5
23	122083.0
24	130738.0
25	165135.5
26	190878.0
27	221634.0
28	252390.0
29	285974.0
30	319558.0
31	352455.0
32	385352.0
33	432205.0
34	479058.0
35	536377.0
36	593696.0
37	652917.0
38	712138.0
39	770282.5
40	828427.0
41	886745.5
42	1001138.0
43	1057212.0
44	1105140.0
45	1153068.0
46	1192282.5
47	1231497.0
48	1252305.5
49	1273114.0
50	1287576.5
51	1302039.0
52	1288871.5
53	1275704.0
54	1232121.5
55	1188539.0
56	1115394.0
57	1042249.0
58	958015.0
59	776625.5
60	679470.0
61	589493.5
62	499517.0
63	420633.5
64	341750.0
65	279741.0
66	217732.0
67	173945.5
68	130159.0
69	100866.5
70	71574.0
71	53755.5
72	35937.0
73	27021.5
74	18106.0
75	13732.5
76	7012.0
77	4665.0
78	3240.5
79	1816.0
80	1353.0
81	890.0
82	644.5
83	399.0
84	251.5
85	104.0
86	70.5
87	37.0
88	31.0
89	25.0
90	18.0
91	11.0
92	8.5
93	5.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.7522446E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.66753758566788
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.74551348899568	35.00243864453054
2	19.585011596997106	20.238186456071787
3	5.5797112065169285	8.64869815439657
4	2.315269286242533	4.784970514715141
5	1.1868101055234421	3.065977786709145
6	0.7125171615539476	2.2088404335013188
7	0.47190768140464595	1.706761550615795
8	0.3471567663084664	1.434938821708929
9	0.26326051001086614	1.2241820066227669
>10	1.6627500210182882	16.14580649506888
>50	0.10823329361609373	3.7773368469582977
>100	0.021695320546864637	1.5119141170741741
>500	7.094455137316905E-5	0.02448560125928169
>1k	6.946688420453378E-5	0.07294255593478095
>5k	1.1574914823897077E-5	0.037706885210355943
>10k+	1.1574914823897077E-5	0.11481312962227017
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19198	0.10956232936885638	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.568134608604301E-4	0.0	0.0	0.0	1.1413931593796894E-5
2	2.568134608604301E-4	5.706965796898447E-6	0.0	0.0	2.2827863187593787E-5
3	2.568134608604301E-4	5.706965796898447E-6	0.0	0.0	3.424179478139068E-5
4	2.568134608604301E-4	1.1413931593796894E-5	0.0	0.0	4.5655726375187574E-5
5	2.625204266573285E-4	2.2827863187593787E-5	0.0	0.0	4.5655726375187574E-5
6	2.68227392454227E-4	2.8534828984492232E-5	0.0	0.0	6.277662376588291E-5
7	2.68227392454227E-4	2.8534828984492232E-5	0.0	0.0	6.848358956278135E-5
8	2.68227392454227E-4	2.8534828984492232E-5	0.0	0.0	6.848358956278135E-5
9	2.68227392454227E-4	2.8534828984492232E-5	0.0	0.0	7.419055535967981E-5
10	2.68227392454227E-4	2.8534828984492232E-5	0.0	0.0	7.989752115657826E-5
11	2.8534828984492236E-4	2.8534828984492232E-5	0.0	0.0	7.989752115657826E-5
12	2.9676222143871925E-4	2.8534828984492232E-5	0.0	5.706965796898447E-6	8.56044869534767E-5
13	2.9676222143871925E-4	2.8534828984492232E-5	0.0	5.706965796898447E-6	1.0843235014107048E-4
14	3.0246918723561767E-4	2.8534828984492232E-5	0.0	1.712089739069534E-5	1.1984628173486739E-4
15	3.0817615303251614E-4	2.8534828984492232E-5	0.0	2.2827863187593787E-5	1.4267414492246118E-4
16	3.1959008462631303E-4	2.8534828984492232E-5	0.0	2.2827863187593787E-5	1.5979504231315652E-4
17	3.2529705042321145E-4	2.8534828984492232E-5	0.0	2.2827863187593787E-5	1.6550200811005496E-4
18	3.538318794077037E-4	2.8534828984492232E-5	0.0	2.2827863187593787E-5	1.712089739069534E-4
19	3.595388452046021E-4	3.424179478139068E-5	0.0	2.2827863187593787E-5	1.826229055007503E-4
20	3.9378063998599284E-4	4.5655726375187574E-5	0.0	2.2827863187593787E-5	1.8832987129764874E-4
21	4.280224347673835E-4	4.5655726375187574E-5	0.0	2.2827863187593787E-5	1.9403683709454719E-4
22	4.565572637518757E-4	4.5655726375187574E-5	0.0	2.2827863187593787E-5	2.0545076868834408E-4
23	5.022129901270633E-4	4.5655726375187574E-5	0.0	2.8534828984492232E-5	2.1115773448524252E-4
24	5.592826480960478E-4	4.5655726375187574E-5	0.0	2.8534828984492232E-5	2.1115773448524252E-4
25	6.220592718619306E-4	4.5655726375187574E-5	0.0	2.8534828984492232E-5	2.339855976728363E-4
26	6.734219640340167E-4	6.277662376588291E-5	0.0	2.8534828984492232E-5	2.453995292666332E-4
27	7.304916220030012E-4	6.277662376588291E-5	0.0	2.8534828984492232E-5	2.739343582511254E-4
28	7.818543141750872E-4	6.277662376588291E-5	0.0	2.8534828984492232E-5	2.796413240480239E-4
29	8.845796985192593E-4	6.277662376588291E-5	0.0	2.8534828984492232E-5	2.8534828984492236E-4
30	9.530632880820406E-4	6.277662376588291E-5	0.0	2.8534828984492232E-5	2.910552556418208E-4
31	0.0010386677750355174	6.277662376588291E-5	0.0	2.8534828984492232E-5	2.910552556418208E-4
32	0.0011585140567703846	6.277662376588291E-5	0.0	2.8534828984492232E-5	3.2529705042321145E-4
33	0.0013011882016928458	6.848358956278135E-5	0.0	2.8534828984492232E-5	3.424179478139068E-4
34	0.0014609832440060024	6.848358956278135E-5	0.0	3.424179478139068E-5	3.424179478139068E-4
35	0.001620778286319159	6.848358956278135E-5	0.0	3.424179478139068E-5	3.766597425952975E-4
36	0.0018490569181950967	7.419055535967981E-5	0.0	3.424179478139068E-5	3.8236670839219595E-4
37	0.002054507686883441	7.419055535967981E-5	0.0	3.424179478139068E-5	3.8807367418909437E-4
38	0.0023056141819469723	7.419055535967981E-5	0.0	3.424179478139068E-5	3.9378063998599284E-4
39	0.002539599779619809	7.419055535967981E-5	0.0	4.5655726375187574E-5	3.9948760578289126E-4
40	0.002784999308886442	7.419055535967981E-5	0.0	4.5655726375187574E-5	3.9948760578289126E-4
41	0.0031787799488724347	7.419055535967981E-5	0.0	5.136269217208602E-5	4.1090153737668815E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	15980	0.0	25.529411	1
CAACGCA	19765	0.0	20.652416	13
TCAACGC	19780	0.0	20.506067	12
AACGCAG	20615	0.0	19.846472	14
ACGCAGA	20970	0.0	19.409634	15
ATCAACG	21045	0.0	19.396294	11
GTATCAA	21385	0.0	19.197802	9
AGTGGTA	21195	0.0	19.170322	5
GGTATCA	21395	0.0	19.11194	8
GTGGTAT	22015	0.0	18.424257	6
TGGTATC	22340	0.0	18.187778	7
CGCAGAG	22600	0.0	18.113716	16
TATCAAC	22730	0.0	18.010118	10
AGCAGTG	23100	0.0	17.894588	2
AGAGTAC	22160	0.0	17.603792	19
GCAGTGG	24935	0.0	16.492882	3
CAGTGGT	25150	0.0	16.249105	4
CAGAGTA	24535	0.0	15.985939	18
GCAGAGT	25955	0.0	15.310536	17
GAGTACT	8925	0.0	14.797758	20
>>END_MODULE