FastQCFastQC Report
Thu 26 May 2016
SRR805402_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805402_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13897420
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2071501.490564435701015No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG258940.18632235335767358No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186410.1341328102626243No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA183770.1322331770933022No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG176000.12664221128813838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT676750.043.336541
AGCAGTG773200.038.0157782
AGTGGTA774150.037.75365
TCAACGC780650.037.6349212
CAACGCA783100.037.61020313
GTATCAA785550.037.3971759
GGTATCA785400.037.3833738
GCAGTGG786000.037.3757633
TGGTATC782400.037.373537
ACGCAGA789550.037.31783715
AACGCAG790850.037.27729814
GTGGTAT784750.037.2436456
CAGTGGT794650.036.844714
ATCAACG801750.036.7880911
CGCAGAG801150.036.76577416
TATCAAC803300.036.59716410
GAGTACT383200.036.29866820
AGAGTAC778750.036.2511119
CAGAGTA792650.035.62726218
GTACTTT389000.035.55809822