##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805402_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13897420
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.937646124244644	37.0	35.0	37.0	35.0	37.0
2	36.01039178495001	37.0	35.0	37.0	35.0	37.0
3	37.75745217457629	39.0	38.0	39.0	35.0	39.0
4	37.73592695622641	39.0	38.0	39.0	35.0	39.0
5	37.658950006547975	39.0	37.0	39.0	35.0	39.0
6	37.57469926072609	39.0	37.0	39.0	35.0	39.0
7	37.52670682759822	39.0	37.0	39.0	35.0	39.0
8	38.54255336602046	40.0	39.0	40.0	36.0	40.0
9	38.51914700714233	40.0	38.0	40.0	36.0	40.0
10	38.424618166537385	40.0	38.0	40.0	35.0	40.0
11	38.48356925242239	40.0	38.0	40.0	35.0	40.0
12	38.46849969274873	40.0	38.0	40.0	35.0	40.0
13	38.54700987665336	40.0	39.0	40.0	35.0	40.0
14	38.55177464594148	40.0	39.0	40.0	35.0	40.0
15	38.54821607176008	40.0	39.0	40.0	35.0	40.0
16	38.50736877780192	40.0	39.0	40.0	35.0	40.0
17	38.44324961035933	40.0	39.0	40.0	35.0	40.0
18	38.33893737110917	40.0	38.0	40.0	34.0	40.0
19	38.33556602592424	40.0	38.0	40.0	34.0	40.0
20	38.2839163672106	40.0	38.0	40.0	34.0	40.0
21	38.21152595229906	40.0	38.0	40.0	34.0	40.0
22	38.19781031299335	40.0	38.0	40.0	34.0	40.0
23	36.86189666859028	40.0	38.0	40.0	32.0	40.0
24	37.367943474400285	40.0	38.0	40.0	32.0	40.0
25	37.74083074412373	40.0	38.0	40.0	32.0	40.0
26	37.760639888554856	40.0	38.0	40.0	33.0	40.0
27	37.87006278863271	40.0	38.0	40.0	34.0	40.0
28	37.9195039079196	40.0	38.0	40.0	34.0	40.0
29	37.75039690820311	40.0	38.0	40.0	33.0	40.0
30	37.775002842254175	40.0	38.0	40.0	33.0	40.0
31	37.62065628008652	40.0	38.0	40.0	33.0	40.0
32	37.74293228527309	40.0	38.0	40.0	33.0	40.0
33	37.4867970457826	40.0	38.0	40.0	33.0	40.0
34	37.66784410343791	40.0	38.0	40.0	33.0	40.0
35	37.729545268114514	40.0	38.0	40.0	33.0	40.0
36	37.69290782030046	40.0	37.0	40.0	33.0	40.0
37	37.451266925803495	40.0	37.0	40.0	33.0	40.0
38	37.46774041512741	40.0	37.0	40.0	33.0	40.0
39	37.520287506601946	40.0	37.0	40.0	33.0	40.0
40	37.49785614883913	40.0	37.0	40.0	33.0	40.0
41	37.40034898563906	40.0	37.0	40.0	33.0	40.0
42	37.33528669350138	40.0	37.0	40.0	33.0	40.0
43	36.523390888380725	40.0	36.0	40.0	30.0	40.0
44	36.65333630270943	40.0	35.0	40.0	30.0	40.0
45	36.86251707151399	40.0	36.0	40.0	31.0	40.0
46	36.89844273253597	40.0	36.0	40.0	31.0	40.0
47	36.90779799416007	40.0	36.0	40.0	31.0	40.0
48	36.89095458005875	39.0	36.0	40.0	31.0	40.0
49	36.69791018764634	39.0	35.0	40.0	31.0	40.0
50	36.57463428463701	39.0	35.0	40.0	31.0	40.0
51	36.35775100702145	39.0	35.0	40.0	30.0	40.0
52	36.26736156783058	39.0	35.0	40.0	30.0	40.0
53	35.83665579654353	38.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	16.0
13	55.0
14	99.0
15	316.0
16	730.0
17	1409.0
18	2873.0
19	5149.0
20	8414.0
21	13309.0
22	20186.0
23	29069.0
24	40384.0
25	54163.0
26	70852.0
27	91041.0
28	115113.0
29	144122.0
30	177619.0
31	220790.0
32	271596.0
33	339395.0
34	439645.0
35	596226.0
36	871537.0
37	1462893.0
38	2335502.0
39	6584912.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	16.24104330156245	35.701417961031616	20.84373934154685	27.21379939585909
2	24.459978902558895	29.272066325979928	26.909807719706247	19.35814705175493
3	23.954144006585395	25.79456474655008	26.150271057505638	24.101020189358888
4	19.506894085376995	27.53350621913996	26.477281394676133	26.482318300806913
5	22.932436380277778	31.006251520066314	26.072796245634084	19.988515854021827
6	26.284511801471066	25.622496837542506	26.93163191441289	21.161359446573535
7	22.729110870938634	26.42811399525955	30.28505290910111	20.5577222247007
8	25.061824424965206	25.523751890638696	27.521986095260846	21.89243758913525
9	25.039510930805864	25.055629030424353	28.52122192464501	21.383638114124782
10	21.300090232575542	26.46090425417092	30.763674120808034	21.475331392445504
11	21.147356847529974	30.620287794425156	26.866288850736325	21.366066507308553
12	21.309602789582527	26.070414508592243	30.616956240798654	22.00302646102658
13	21.474028992431688	26.664632716000526	25.768725418099187	26.092612873468603
14	21.847630711311883	30.781756613817528	25.739748816686834	21.630863858183748
15	21.654400600974856	30.393965210808915	25.698129580886235	22.253504607329994
16	22.300081597879316	26.137196688306176	25.36223270218501	26.200489011629497
17	26.698890873270003	25.76427135396354	25.598614706902435	21.938223065864022
18	22.151572018403414	26.12815903959152	25.41845177018468	26.301817171820378
19	22.45589469124485	30.457459010377463	25.29533539318809	21.791310905189594
20	26.5953896478627	26.20545396195841	25.57826560613409	21.620890784044807
21	22.48427405950169	30.162066052547882	25.615200519233067	21.738459368717358
22	26.276754966029664	25.940548677380406	25.869074979384663	21.913621377205267
23	21.43363300526285	28.457886427840563	29.221315898922246	20.887164667974343
24	21.833512982985333	30.140752744034504	25.693553191887414	22.33218108109275
25	21.867828704896304	26.14796127626567	25.676384537561646	26.30782548127638
26	22.28107807060591	27.791568506960285	28.432378096078263	21.49497532635554
27	22.326611702028146	25.523320155827484	30.321714390152994	21.82835375199138
28	24.476298478422613	25.81784244845446	27.867704940917093	21.838154132205833
29	24.584404875149488	25.64699778807865	28.0097672805456	21.758830056226262
30	24.391052439949284	25.594772267082668	28.03667155486414	21.977503738103906
31	23.794826665668882	26.11847378866005	27.95990910543108	22.126790440239986
32	23.502096072508422	26.14960186854826	28.263433068871773	22.084868990071538
33	23.287739738742875	26.00426553993475	28.230340595592562	22.477654125729813
34	22.87437524375028	26.24730345632499	28.197715835025495	22.68060546489924
35	22.732672683131113	26.080272453448195	28.706688003960444	22.480366859460247
36	22.918232304988983	26.050389208932305	28.569266813552442	22.462111672526266
37	23.328027792208914	26.308703342059175	28.0077453225131	22.355523543218816
38	23.073843922109283	26.34878991927998	28.502909173069536	22.074456985541204
39	22.995721508020914	26.306335996177708	28.405423452698415	22.292519043102963
40	22.839699742829964	26.77378247185449	28.027597928248554	22.358919857066994
41	22.92281588956799	26.624243924411868	28.320270956767512	22.132669229252624
42	22.802433833042393	26.574450509519032	28.329430930345346	22.29368472709323
43	22.784819052745043	26.439087255044463	28.38836273207545	22.38773096013505
44	23.1653213330244	26.17539802351803	28.42464284737743	22.234637796080136
45	23.06339593967801	26.069882035658416	28.453158931657818	22.413563093005752
46	22.814709492841118	26.307552049229283	28.178287768521066	22.699450689408536
47	22.688959533496146	26.255125051988067	28.467586069932405	22.588329344583382
48	22.533592566102197	26.22639310030207	28.396177132158346	22.84383720143739
49	22.60724652489455	26.790382675345498	27.995692725700167	22.60667807405979
50	23.06161143579168	26.63119485487234	28.07539816742964	22.23179554190634
51	22.926507222203835	26.43582765721983	28.28468161716347	22.35298350341286
52	23.053739471067292	26.60430497171418	27.842829820211236	22.4991257370073
53	23.370776734098847	26.606168626982562	27.678079816253664	22.344974822664927
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20502.0
1	13544.0
2	6586.0
3	5689.0
4	4792.0
5	4802.0
6	4812.0
7	5057.0
8	5783.0
9	6264.0
10	7253.0
11	8242.0
12	10145.0
13	12048.0
14	14019.5
15	15991.0
16	21436.5
17	26882.0
18	35666.0
19	44450.0
20	59686.5
21	74923.0
22	219840.5
23	364758.0
24	276756.0
25	208308.5
26	227863.0
27	258211.0
28	288559.0
29	317554.0
30	346549.0
31	365618.0
32	384687.0
33	416301.5
34	447916.0
35	503358.5
36	558801.0
37	608122.0
38	657443.0
39	692560.5
40	727678.0
41	756713.5
42	805416.5
43	825084.0
44	836951.0
45	848818.0
46	858340.0
47	867862.0
48	881216.0
49	894570.0
50	896033.0
51	897496.0
52	883035.0
53	868574.0
54	841786.0
55	814998.0
56	783093.0
57	751188.0
58	674080.0
59	520523.5
60	444075.0
61	384001.5
62	323928.0
63	276190.5
64	228453.0
65	186045.0
66	143637.0
67	114296.0
68	84955.0
69	65942.0
70	46929.0
71	35894.5
72	24860.0
73	18640.5
74	12421.0
75	9464.5
76	4949.5
77	3391.0
78	2509.0
79	1627.0
80	1239.0
81	851.0
82	627.5
83	404.0
84	269.0
85	134.0
86	97.5
87	61.0
88	50.5
89	40.0
90	27.5
91	15.0
92	13.5
93	8.5
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.389742E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.20604568603095
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.0664852571534	20.794973784766164
2	19.069207110804847	13.427027534787634
3	8.57825807913731	9.060196375220166
4	4.409131008507262	6.209122708848095
5	2.4061456496368585	4.235543683417993
6	1.3680108146586647	2.889735074391442
7	0.9054699594278477	2.2314611731261778
8	0.6547979719246172	1.8442277851758786
9	0.49595842648911154	1.5714661519212918
>10	2.6717205091986442	16.960433179976086
>50	0.17794519676737675	4.408091692398809
>100	0.19406515069217029	13.197106816353033
>500	0.0019514342185484189	0.4119217108225366
>1k	6.869123110309695E-4	0.48425602619219876
>5k	6.244465209446127E-5	0.16642109607858352
>10k+	1.0407442015743546E-4	2.1080152065238877
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	207150	1.490564435701015	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	25894	0.18632235335767358	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18641	0.1341328102626243	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18377	0.1322331770933022	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	17600	0.12664221128813838	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.662364669125636E-4	0.0	0.0	0.0	7.1955801868260436E-6
2	2.662364669125636E-4	0.0	0.0	0.0	1.4391160373652087E-5
3	2.662364669125636E-4	0.0	0.0	1.4391160373652087E-5	1.4391160373652087E-5
4	2.7343204709938965E-4	0.0	0.0	1.4391160373652087E-5	1.4391160373652087E-5
5	2.950187876598678E-4	0.0	0.0	1.4391160373652087E-5	1.4391160373652087E-5
6	2.950187876598678E-4	0.0	0.0	1.4391160373652087E-5	2.158674056047813E-5
7	2.950187876598678E-4	0.0	0.0	1.4391160373652087E-5	2.158674056047813E-5
8	2.950187876598678E-4	0.0	0.0	1.4391160373652087E-5	2.158674056047813E-5
9	2.950187876598678E-4	0.0	0.0	1.4391160373652087E-5	2.158674056047813E-5
10	2.950187876598678E-4	0.0	0.0	1.4391160373652087E-5	2.8782320747304174E-5
11	2.950187876598678E-4	0.0	0.0	1.4391160373652087E-5	2.8782320747304174E-5
12	2.950187876598678E-4	0.0	0.0	3.597790093413022E-5	3.597790093413022E-5
13	3.0221436784669384E-4	0.0	0.0	3.597790093413022E-5	3.597790093413022E-5
14	3.0940994803351987E-4	0.0	0.0	1.295204433628688E-4	3.597790093413022E-5
15	3.1660552822034596E-4	0.0	0.0	1.3671602354969482E-4	3.597790093413022E-5
16	3.23801108407172E-4	0.0	0.0	1.3671602354969482E-4	3.597790093413022E-5
17	3.3819226878082406E-4	0.0	0.0	1.3671602354969482E-4	4.317348112095626E-5
18	3.813657499017803E-4	0.0	0.0	1.3671602354969482E-4	6.47602216814344E-5
19	4.0295249046225847E-4	0.0	0.0	1.3671602354969482E-4	6.47602216814344E-5
20	4.101480706490845E-4	0.0	0.0	1.3671602354969482E-4	6.47602216814344E-5
21	4.389303913963887E-4	0.0	0.0	1.3671602354969482E-4	7.915138205508649E-5
22	4.605171319568668E-4	0.0	0.0	1.5830276411017298E-4	7.915138205508649E-5
23	5.180817734514752E-4	0.0	0.0	1.5830276411017298E-4	7.915138205508649E-5
24	5.612552545724315E-4	0.0	0.0	1.65498344296999E-4	7.915138205508649E-5
25	6.188198960670397E-4	0.0	0.0	1.65498344296999E-4	8.634696224191252E-5
26	6.47602216814344E-4	0.0	0.0	1.65498344296999E-4	1.0073812261556462E-4
27	6.907756979353002E-4	0.0	0.0	1.65498344296999E-4	1.0073812261556462E-4
28	7.267535988694304E-4	0.0	0.0	1.65498344296999E-4	1.0073812261556462E-4
29	7.987094007376908E-4	0.0	0.0	1.7269392448382505E-4	1.151292829892167E-4
30	8.706652026059513E-4	0.0	0.0	1.8708508485747714E-4	1.151292829892167E-4
31	9.210342639137336E-4	0.0	0.0	1.8708508485747714E-4	1.295204433628688E-4
32	0.0010577502874634284	0.0	0.0	1.942806650443032E-4	1.295204433628688E-4
33	0.001323986754375992	0.0	0.0	1.942806650443032E-4	1.3671602354969482E-4
34	0.0014391160373652088	0.0	0.0	2.1586740560478133E-4	1.3671602354969482E-4
35	0.001626201122222686	0.0	0.0	2.2306298579160736E-4	1.3671602354969482E-4
36	0.0017773083061460328	0.0	0.0	2.2306298579160736E-4	1.3671602354969482E-4
37	0.0019284154900693798	0.0	0.0	2.2306298579160736E-4	1.798895046706511E-4
38	0.002108304994740031	0.0	0.0	2.3745414616525945E-4	1.942806650443032E-4
39	0.0023457591409052903	0.0	0.0	2.3745414616525945E-4	2.1586740560478133E-4
40	0.002633582348378332	0.0	0.0	2.3745414616525945E-4	2.302585659784334E-4
41	0.0028422541737962875	0.0	0.0	2.5184530653891155E-4	2.302585659784334E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	67675	0.0	43.33654	1
AGCAGTG	77320	0.0	38.015778	2
AGTGGTA	77415	0.0	37.7536	5
TCAACGC	78065	0.0	37.63492	12
CAACGCA	78310	0.0	37.610203	13
GTATCAA	78555	0.0	37.397175	9
GGTATCA	78540	0.0	37.383373	8
GCAGTGG	78600	0.0	37.375763	3
TGGTATC	78240	0.0	37.37353	7
ACGCAGA	78955	0.0	37.317837	15
AACGCAG	79085	0.0	37.277298	14
GTGGTAT	78475	0.0	37.243645	6
CAGTGGT	79465	0.0	36.84471	4
ATCAACG	80175	0.0	36.78809	11
CGCAGAG	80115	0.0	36.765774	16
TATCAAC	80330	0.0	36.597164	10
GAGTACT	38320	0.0	36.298668	20
AGAGTAC	77875	0.0	36.25111	19
CAGAGTA	79265	0.0	35.627262	18
GTACTTT	38900	0.0	35.558098	22
>>END_MODULE