FastQCFastQC Report
Thu 26 May 2016
SRR805401_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805401_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16817861
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3357511.9963953798880842No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG406120.24148136317692243No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG311500.18521974940808467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1524500.044.18371
TCAACGC1561650.043.1025212
CAACGCA1566700.043.04308313
GTATCAA1566850.042.9474759
GTGGTAT1562150.042.9443056
AGTGGTA1563250.042.882525
GGTATCA1568550.042.8769578
ATCAACG1576250.042.85833411
AACGCAG1576450.042.7932714
TGGTATC1567750.042.7699287
ACGCAGA1577700.042.73553515
TATCAAC1582800.042.55032310
CGCAGAG1589150.042.40116
AGCAGTG1601400.042.177912
GCAGTGG1598200.042.1594283
AGAGTAC1530350.042.02466619
CAGTGGT1598800.041.9775124
GAGTACT580700.041.59342620
CAGAGTA1547500.041.58019318
GCAGAGT1564600.041.3074917