##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805401_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16817861
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.1054987908391	37.0	35.0	37.0	35.0	37.0
2	36.121574021809316	37.0	35.0	37.0	35.0	37.0
3	37.87120758103542	39.0	38.0	39.0	35.0	39.0
4	37.82063474064865	39.0	38.0	39.0	35.0	39.0
5	37.72142652386056	39.0	38.0	39.0	35.0	39.0
6	37.66037958097049	39.0	37.0	39.0	35.0	39.0
7	37.54687977264172	39.0	37.0	39.0	35.0	39.0
8	38.59407001877349	40.0	39.0	40.0	36.0	40.0
9	38.594346034849494	40.0	39.0	40.0	36.0	40.0
10	38.35664321402109	40.0	38.0	40.0	35.0	40.0
11	38.484070358293486	40.0	38.0	40.0	36.0	40.0
12	38.5232367540676	40.0	39.0	40.0	36.0	40.0
13	38.62472855495714	40.0	39.0	40.0	36.0	40.0
14	38.63215446958445	40.0	39.0	40.0	36.0	40.0
15	38.63885948397362	40.0	39.0	40.0	36.0	40.0
16	38.58380610946897	40.0	39.0	40.0	36.0	40.0
17	38.52686283945384	40.0	39.0	40.0	35.0	40.0
18	38.44265474664108	40.0	39.0	40.0	35.0	40.0
19	38.43611146506681	40.0	38.0	40.0	35.0	40.0
20	38.4084125799351	40.0	38.0	40.0	35.0	40.0
21	38.304242673904845	40.0	38.0	40.0	35.0	40.0
22	38.33618490484611	40.0	38.0	40.0	35.0	40.0
23	36.883189247431645	40.0	38.0	40.0	32.0	40.0
24	37.44584284529406	40.0	38.0	40.0	33.0	40.0
25	37.86066272042562	40.0	38.0	40.0	33.0	40.0
26	37.89375634630349	40.0	38.0	40.0	34.0	40.0
27	38.006950705562375	40.0	38.0	40.0	34.0	40.0
28	38.04341283353454	40.0	38.0	40.0	34.0	40.0
29	37.879346606563104	40.0	38.0	40.0	34.0	40.0
30	37.909436164325534	40.0	38.0	40.0	34.0	40.0
31	37.76990676757288	40.0	38.0	40.0	33.0	40.0
32	37.886060302198956	40.0	38.0	40.0	33.0	40.0
33	37.60962984531743	40.0	38.0	40.0	33.0	40.0
34	37.796187041859845	40.0	38.0	40.0	33.0	40.0
35	37.84652542912562	40.0	38.0	40.0	33.0	40.0
36	37.86385052177563	40.0	38.0	40.0	33.0	40.0
37	37.59105096658844	40.0	37.0	40.0	33.0	40.0
38	37.55949909444489	40.0	37.0	40.0	33.0	40.0
39	37.61805654119748	40.0	37.0	40.0	33.0	40.0
40	37.583800936397324	40.0	37.0	40.0	33.0	40.0
41	37.51679110678819	40.0	37.0	40.0	33.0	40.0
42	37.452298065729046	40.0	37.0	40.0	33.0	40.0
43	36.66614577204557	40.0	36.0	40.0	30.0	40.0
44	36.79263171458011	40.0	36.0	40.0	31.0	40.0
45	36.99378803285388	40.0	36.0	40.0	31.0	40.0
46	37.01538947194295	40.0	36.0	40.0	32.0	40.0
47	37.02425254912025	40.0	36.0	40.0	32.0	40.0
48	37.00754055465199	40.0	36.0	40.0	32.0	40.0
49	36.81549276688635	39.0	35.0	40.0	31.0	40.0
50	36.69619097220509	39.0	35.0	40.0	31.0	40.0
51	36.441965122675235	39.0	35.0	40.0	30.0	40.0
52	36.35592231378295	39.0	35.0	40.0	30.0	40.0
53	35.91714695465731	38.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	11.0
13	18.0
14	92.0
15	234.0
16	543.0
17	1270.0
18	2535.0
19	4795.0
20	8170.0
21	13374.0
22	20425.0
23	29962.0
24	42429.0
25	57764.0
26	76966.0
27	99112.0
28	126397.0
29	158692.0
30	199056.0
31	246670.0
32	307372.0
33	391510.0
34	510990.0
35	711218.0
36	1051487.0
37	1795291.0
38	2856485.0
39	8104991.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.575134317021647	38.446381498812485	28.007372637935347	21.971111546230524
2	27.90733613507687	42.52664473799611	13.7847851162523	15.781234010674721
3	34.09638716838009	26.21572386642986	19.246603358179733	20.44128560701031
4	21.56450216826028	24.77917970662262	20.698095911245787	32.958222213871316
5	20.4367071412946	33.554570346371634	24.130851123100612	21.87787138923315
6	30.208990310955713	24.007916345604237	22.665617226828072	23.117476116611975
7	22.642082723837472	23.537309530623425	31.82414814820981	21.99645959732929
8	29.533571480939223	25.212629596593768	23.911120445102977	21.342678477364036
9	29.89444377022738	24.458889272541853	24.25577783048629	21.39088912674448
10	21.3820949049347	25.105011868037202	32.129573433862966	21.383319793165136
11	21.118981777765914	33.39156507477378	24.14169078933403	21.34776235812628
12	21.115093054937248	24.730760945164192	32.518564637916796	21.635581361981764
13	21.11481359014681	25.026321718320776	24.094479077927925	29.76438561360449
14	21.497733867582806	33.547060473386004	23.734445183011086	21.220760476020107
15	21.262519650982963	33.16874839196257	23.77863035019733	21.79010160685714
16	21.651278958721328	24.49018338301167	23.97690169992486	29.881635958342144
17	30.062990769159047	24.39815027606662	23.955317504407965	21.58354145036637
18	21.618325897686987	24.537965916117393	23.9345062966093	29.909201889586317
19	21.73485082318138	32.93710181098536	23.890933573538277	21.43711379229499
20	30.232721033905563	24.736599975466557	23.91478321767554	21.11589577295234
21	21.86964204306362	32.81510056481024	23.922893642657648	21.392363749468498
22	29.858386866201357	24.314298946816127	24.35707489793143	21.470239289051086
23	21.127567887497705	26.846630495994706	31.40963645733545	20.616165159172144
24	21.602610462769313	32.560335704998394	24.037932053309277	21.799121778923013
25	21.45411357603681	24.533286367392382	24.072550010967504	29.9400500456033
26	21.956751812849447	29.510114276720444	27.28984976151248	21.24328414891763
27	21.967526072429784	24.205830931769505	32.42094223516296	21.405700760637753
28	27.011639589600605	24.23921805513793	27.249541425036156	21.499600930225313
29	27.154047711537153	24.34825689188417	27.036642769255852	21.46105262732282
30	27.091840038397276	24.229127592385264	27.117747019076923	21.561285350140544
31	25.267666322132165	24.982083036600194	27.635137429189122	22.115113212078516
32	24.270369460182838	25.723419880804105	27.80992184440102	22.196288814612036
33	24.0226268964882	25.20469160733342	28.116899051550014	22.655782444628365
34	23.267524924840323	25.26222567780766	28.405027250492793	23.065222146859224
35	23.04730666997426	25.382722570961906	28.679568703772734	22.890402055291098
36	23.17411827818056	25.321983574486673	28.6921029969269	22.81179515040587
37	23.801974579288053	25.191081077433093	28.49049590789221	22.51644843538664
38	23.680032793706644	25.417287014085797	28.459326664669188	22.44335352753837
39	23.584319076010914	25.26758307730097	28.521938669846303	22.626159176841814
40	23.17634210438533	25.758929747367993	28.286629316296523	22.77809883195015
41	23.51357286161421	25.962100650017266	28.074557162768798	22.449769325599732
42	23.425743618644486	25.866303687490344	28.19048153626671	22.51747115759846
43	23.26410594070197	25.59191088569468	28.61101063922457	22.53297253437878
44	23.66035728324785	25.488051066660617	28.3642610674449	22.487330582646628
45	23.672635895849062	25.110327645114914	28.385316063677777	22.831720395358243
46	23.382129273157865	25.21252851358446	28.2049007302415	23.20044148301618
47	23.29353893458865	25.338406590469503	28.314474712331133	23.053579762610717
48	23.06884924307556	25.189749160133978	28.4172047800847	23.324196816705765
49	22.8866441457686	25.751883667013303	28.212672229839452	23.148799957378646
50	23.61702834861104	25.734194140384442	27.924764035093403	22.724013475911118
51	23.529181267463205	25.481409318343157	28.161387467764182	22.828021946429452
52	23.62900371218433	25.444145364264813	28.014751697614816	22.912099225936046
53	24.04902740009565	25.594919591736424	27.51769681055159	22.83835619761633
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2665.0
1	1738.0
2	811.0
3	699.0
4	587.0
5	647.5
6	708.0
7	810.5
8	1153.5
9	1394.0
10	2244.0
11	3094.0
12	4677.5
13	6261.0
14	8410.5
15	10560.0
16	15052.5
17	19545.0
18	26883.0
19	34221.0
20	48572.5
21	62924.0
22	276721.5
23	490519.0
24	353623.5
25	219396.0
26	222064.0
27	243839.5
28	265615.0
29	293382.0
30	321149.0
31	348194.5
32	375240.0
33	417990.5
34	460741.0
35	515331.5
36	569922.0
37	624438.0
38	678954.0
39	730776.0
40	782598.0
41	835445.0
42	936587.5
43	984883.0
44	1031608.5
45	1078334.0
46	1108388.5
47	1138443.0
48	1161990.5
49	1185538.0
50	1187554.0
51	1189570.0
52	1175105.5
53	1160641.0
54	1129441.0
55	1098241.0
56	1065931.0
57	1033621.0
58	919206.0
59	699468.0
60	594145.0
61	512812.0
62	431479.0
63	361986.5
64	292494.0
65	237891.5
66	183289.0
67	145347.0
68	107405.0
69	83184.0
70	58963.0
71	44817.5
72	30672.0
73	22871.5
74	15071.0
75	11347.0
76	5610.5
77	3598.0
78	2679.0
79	1760.0
80	1385.5
81	1011.0
82	734.0
83	457.0
84	321.5
85	186.0
86	125.5
87	65.0
88	52.0
89	39.0
90	31.0
91	23.0
92	15.0
93	5.0
94	3.0
95	2.0
96	1.0
97	2.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.6817861E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.75910249654953
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.3305003415743	35.49341677626556
2	15.699326169101735	15.62368759950191
3	5.294026458648298	7.902780155259774
4	2.3749166890695985	4.726948918087208
5	1.2976114074868503	3.2283989512915037
6	0.8144621026023962	2.431614194576872
7	0.5388103057342475	1.8767502060459322
8	0.3773958237558325	1.502310198082897
9	0.3156186555052411	1.4134410926197603
>10	1.8268046077645503	16.110903853956934
>50	0.09631224685381473	3.2891491222360325
>100	0.032701228171855945	2.58011287893426
>500	8.368234535825196E-4	0.29321875353410964
>1k	5.294215240607131E-4	0.479988743792335
>5k	6.154948068570964E-5	0.21685990297325755
>10k+	8.616927295999347E-5	2.83041865284159
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	335751	1.9963953798880842	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	40612	0.24148136317692243	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	31150	0.18521974940808467	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2486724679196717E-4	5.946059371046056E-6	0.0	5.946059371046056E-6	1.1892118742092113E-5
2	0.0040314282535692265	5.946059371046056E-6	0.0	5.946059371046056E-6	1.1892118742092113E-5
3	0.0040492664316823645	5.946059371046056E-6	0.0	5.946059371046056E-6	3.5676356226276337E-5
4	0.0042217021534427	1.1892118742092113E-5	0.0	1.1892118742092113E-5	3.5676356226276337E-5
5	0.004227648212813746	1.1892118742092113E-5	0.0	1.7838178113138168E-5	5.351453433941451E-5
6	0.004227648212813746	1.7838178113138168E-5	0.0	2.3784237484184226E-5	5.351453433941451E-5
7	0.004227648212813746	1.7838178113138168E-5	0.0	2.3784237484184226E-5	5.351453433941451E-5
8	0.004227648212813746	1.7838178113138168E-5	0.0	2.3784237484184226E-5	5.351453433941451E-5
9	0.004227648212813746	2.9730296855230283E-5	0.0	2.3784237484184226E-5	5.351453433941451E-5
10	0.004245486390926884	3.5676356226276337E-5	0.0	2.3784237484184226E-5	5.351453433941451E-5
11	0.00425143245029793	3.5676356226276337E-5	0.0	2.9730296855230283E-5	6.540665308150662E-5
12	0.004310893044008391	3.5676356226276337E-5	0.0	8.324483119464479E-5	6.540665308150662E-5
13	0.004316839103379437	3.5676356226276337E-5	0.0	8.324483119464479E-5	6.540665308150662E-5
14	0.004352515459605713	3.5676356226276337E-5	0.0	1.0108300930778296E-4	6.540665308150662E-5
15	0.004382245756460943	3.5676356226276337E-5	0.0	1.1892118742092113E-4	7.135271245255267E-5
16	0.004411976053316174	3.5676356226276337E-5	0.0	1.2486724679196717E-4	7.135271245255267E-5
17	0.004423868172058266	3.5676356226276337E-5	0.0	1.3081330616301323E-4	7.135271245255267E-5
18	0.004429814231429312	3.5676356226276337E-5	0.0	1.4270542490510535E-4	7.729877182359873E-5
19	0.004441706350171404	3.5676356226276337E-5	5.946059371046056E-6	1.4270542490510535E-4	9.51369499367369E-5
20	0.004465490587655588	3.5676356226276337E-5	5.946059371046056E-6	1.486514842761514E-4	1.0702906867882902E-4
21	0.004501166943881865	3.5676356226276337E-5	5.946059371046056E-6	1.486514842761514E-4	1.0702906867882902E-4
22	0.004513059062623957	3.5676356226276337E-5	5.946059371046056E-6	1.7243572176033563E-4	1.1892118742092113E-4
23	0.004524951181366049	3.5676356226276337E-5	5.946059371046056E-6	1.8432784050242775E-4	1.3081330616301323E-4
24	0.004572519656334417	4.1622415597322394E-5	5.946059371046056E-6	1.8432784050242775E-4	1.3081330616301323E-4
25	0.004608196012560694	4.1622415597322394E-5	5.946059371046056E-6	1.8432784050242775E-4	1.3081330616301323E-4
26	0.0046736026656422	4.1622415597322394E-5	5.946059371046056E-6	1.9621995924451986E-4	1.3081330616301323E-4
27	0.004697386903126385	4.1622415597322394E-5	5.946059371046056E-6	2.0811207798661198E-4	1.3081330616301323E-4
28	0.004733063259352661	4.756847496836845E-5	5.946059371046056E-6	2.0811207798661198E-4	1.367593655340593E-4
29	0.004822254149918352	4.756847496836845E-5	5.946059371046056E-6	2.0811207798661198E-4	1.367593655340593E-4
30	0.00486982262488672	4.756847496836845E-5	5.946059371046056E-6	2.0811207798661198E-4	1.4270542490510535E-4
31	0.004982797752936596	4.756847496836845E-5	5.946059371046056E-6	2.0811207798661198E-4	1.4270542490510535E-4
32	0.005071988643502286	4.756847496836845E-5	5.946059371046056E-6	2.1405813735765803E-4	1.4270542490510535E-4
33	0.0051909098309232075	4.756847496836845E-5	5.946059371046056E-6	2.200041967287041E-4	1.486514842761514E-4
34	0.005286046780859944	4.756847496836845E-5	5.946059371046056E-6	2.200041967287041E-4	1.486514842761514E-4
35	0.005446590383878187	4.756847496836845E-5	5.946059371046056E-6	2.2595025609975015E-4	1.6054360301824352E-4
36	0.005553619452557016	5.351453433941451E-5	5.946059371046056E-6	2.2595025609975015E-4	1.6648966238928958E-4
37	0.005642810343122707	6.540665308150662E-5	5.946059371046056E-6	2.2595025609975015E-4	1.783817811313817E-4
38	0.005737947293059444	6.540665308150662E-5	5.946059371046056E-6	2.2595025609975015E-4	1.783817811313817E-4
39	0.005892544836706642	6.540665308150662E-5	5.946059371046056E-6	2.2595025609975015E-4	1.783817811313817E-4
40	0.006094710855322208	6.540665308150662E-5	5.946059371046056E-6	2.2595025609975015E-4	1.8432784050242775E-4
41	0.0063622835270192805	7.135271245255267E-5	5.946059371046056E-6	2.2595025609975015E-4	2.1405813735765803E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	152450	0.0	44.1837	1
TCAACGC	156165	0.0	43.10252	12
CAACGCA	156670	0.0	43.043083	13
GTATCAA	156685	0.0	42.947475	9
GTGGTAT	156215	0.0	42.944305	6
AGTGGTA	156325	0.0	42.88252	5
GGTATCA	156855	0.0	42.876957	8
ATCAACG	157625	0.0	42.858334	11
AACGCAG	157645	0.0	42.79327	14
TGGTATC	156775	0.0	42.769928	7
ACGCAGA	157770	0.0	42.735535	15
TATCAAC	158280	0.0	42.550323	10
CGCAGAG	158915	0.0	42.401	16
AGCAGTG	160140	0.0	42.17791	2
GCAGTGG	159820	0.0	42.159428	3
AGAGTAC	153035	0.0	42.024666	19
CAGTGGT	159880	0.0	41.977512	4
GAGTACT	58070	0.0	41.593426	20
CAGAGTA	154750	0.0	41.580193	18
GCAGAGT	156460	0.0	41.30749	17
>>END_MODULE