FastQCFastQC Report
Thu 26 May 2016
SRR805400_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805400_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15701452
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGTA43850.034.62029610
TATGCCG44500.033.9561815
CTCGTAT45800.032.8897411
TCGTATG47750.031.64502512
CGTATGC51450.029.68901813
ATGCCGT50400.029.37516
CCGTCTT53100.027.88135519
TGCCGTC53550.027.6470617
GCCGTCT53300.027.64446418
GAGCTCG56400.027.1666668
ATCGGAA57300.026.6989541
AAGCAGT152800.024.8687821
AGCTCGT63600.024.1281459
GTATGCC67400.022.97700114
CAACGCA174150.022.42721713
TCAACGC173900.022.41891912
AACGCAG179550.021.83124514
ACGCAGA181850.021.49051315
ATCAACG184600.021.2340211
CGTCTTC71400.020.86694720
Produced by FastQC (version 0.11.5)